Leica Stellaris 8 Confocal Microscope

Leica Stellaris 8 共焦显微镜

• 批准号: 10431426
• 负责人: HANS-WILLEM E SNOECK
• 金额: $ 65.16万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431426
• 关键词: Antibodies; Complement; Confocal Microscopy; Detection; Development; Microscope; Resolution; Scanning; Tissue imaging; antibody conjugate; cellular imaging; detector; fluorophore; genome-wide; human disease; imaging system; innovation; multiplexed imaging; next generation sequencing; stem cell biology; tool; user-friendly

Conventional confocal microscopy is a powerful tool for special-temporal cellular and tissue imaging, typically with up to four fluorophores. Recently, there has been an increasing demand for multiplex, high-resolution analysis. However, with most currently available microscopes it is technically impossible to detect fluorophores in the near-infrared (NIR) range, thus limiting the multiplexing capability. The enhanced detection is required not only for detection in the near- infrared (NIR), but also because this allows the use of directly conjugated antibodies when the choice of species in which any primary antibodies were generated is limited. Establishing a user- friendly multiplex imaging system to complement genome-wide, next-generation sequencing analysis is an unmet need. An additional limitation of conventional confocal microscopy is its resolution. Several recently developed confocal scanning microscopes are beginning to meet this need by combining superior detectors and advanced computational deconvolution to enhance both multiplexing options and resolution. Both multiplexing capability and resolution are particularly imperative for the major users on this proposal who focus on developmental and stem cell biology to deepen our understanding of human disease and develop innovative treatments. We therefore propose to acquire a Leica Stellaris 8 microscope that allows multiplexing by providing excitation and enhanced detection in the NIR, as well as computational super- resolution.

传统的共聚焦显微镜是一种强有力的工具，用于特殊时间的细胞和组织 成像，通常使用多达四个荧光团。最近， 用于多重高分辨率分析。然而，对于目前大多数可用的显微镜， 技术上不可能检测近红外（NIR）范围内的荧光团，因此限制了 多路复用能力增强的检测不仅需要用于近距离检测， 这不仅是因为这允许使用近红外（NIR），而且还因为这允许在使用直接缀合的抗体时使用直接缀合的抗体。 产生任何一抗的物种的选择是有限的。建立用户- 友好的多重成像系统，以补充全基因组，下一代测序 分析是一个未满足的需求。常规共焦显微镜的另一个限制是其 分辨率最近开发的几种共焦扫描显微镜开始满足这一点 需要通过结合上级检测器和先进的计算反卷积来增强 多路复用选项和分辨率。多路复用能力和分辨率均 对于关注发展和科学、技术、工程和管理的主要用户来说， 细胞生物学，以加深我们对人类疾病的理解，并开发创新的治疗方法。 因此，我们建议购买一台Leica Stellaris 8显微镜，可以通过以下方式进行多路复用 提供激发和增强的近红外检测，以及计算超级， 分辨率