Leica Stellaris 8 Confocal Microscope

传统的共聚焦显微镜是一种研究特殊时间段细胞和组织的有力工具 成像，通常最多有四个荧光团。最近，需求一直在增加。 用于多路、高分辨率分析。然而，对于目前可用的大多数显微镜来说，它是 从技术上讲，在近红外(NIR)范围内无法检测到荧光团，因此限制了 多路复用能力。增强的检测不仅是为了在近距离- 红外(NIR)，但也因为这允许使用直接结合的抗体时 产生初级抗体的物种选择是有限的。建立用户- 友好的多路成像系统，以补充全基因组、下一代测序 分析是一种未得到满足的需求。传统共聚焦显微镜的另一个局限性是它的 决议。最近开发的几种共焦扫描显微镜开始满足这一要求 需要结合卓越的探测器和高级计算反卷积来增强 多路传输选项和分辨率。多路复用能力和分辨率都是 对于本提案的主要用户来说，尤其迫切需要关注发展和干预性 细胞生物学，以加深我们对人类疾病的理解，并开发创新的治疗方法。 因此，我们建议购买徕卡Stellaris 8显微镜，使其能够通过 在近红外光谱中提供激发和增强的检测，以及计算超级 决议。

Conventional confocal microscopy is a powerful tool for special-temporal cellular and tissue imaging, typically with up to four fluorophores. Recently, there has been an increasing demand for multiplex, high-resolution analysis. However, with most currently available microscopes it is technically impossible to detect fluorophores in the near-infrared (NIR) range, thus limiting the multiplexing capability. The enhanced detection is required not only for detection in the near- infrared (NIR), but also because this allows the use of directly conjugated antibodies when the choice of species in which any primary antibodies were generated is limited. Establishing a user- friendly multiplex imaging system to complement genome-wide, next-generation sequencing analysis is an unmet need. An additional limitation of conventional confocal microscopy is its resolution. Several recently developed confocal scanning microscopes are beginning to meet this need by combining superior detectors and advanced computational deconvolution to enhance both multiplexing options and resolution. Both multiplexing capability and resolution are particularly imperative for the major users on this proposal who focus on developmental and stem cell biology to deepen our understanding of human disease and develop innovative treatments. We therefore propose to acquire a Leica Stellaris 8 microscope that allows multiplexing by providing excitation and enhanced detection in the NIR, as well as computational super- resolution.