600MHz NMR Spectrometer

项目摘要 我们计划购买一台Bruker Avance Neo 2通道600 MHz核磁共振光谱仪， BBFO CryoProbe和SampleCase自动化设备将安装在核磁共振设施中，地址为 东北大学巴尼特研究所。这种多功能的配置将使 我们的核磁共振核心中的两个老化和超订用仪器的功能，500 MHz 瓦里安INOVA和400 MHz瓦里安水星Plus，以满足快速增长的需求 化学和生物结构分析。 所要求的600 MHz核磁共振的尖端CryoProbe和自动化功能将 使一系列创新项目走在跨学科生物医学研究的前沿， 将常规使用留给现有的核磁共振仪器。美国国立卫生研究院资助的以下项目 该仪器的主要用户将立即受益于 仪器：动态靶向合成以调节蛋白质-蛋白质相互作用(R. 发现用于探测和调节原生动物的铅和工具化合物 寄生虫生物学(M.Pollastri/L.Ferrins)；生物活性天然产物的从头合成 产品和碳水化合物基序(G.A.O‘Doherty)；目标是 治疗脑部疾病(A.Makriyannis)；分析细胞提取物中的次生代谢物 未培养的细菌(K.刘易斯)；通过立体结构改进寡核苷酸生物药物 细菌DNA复制中的过程性钳制蛋白的动力学(P. 以及生物活性分子跨越紧密的上皮屏障的运输(W. Lencer，哈佛医学院)。

Project Summary We propose to acquire a Bruker Avance Neo 2-channel 600 MHz NMR spectrometer with a BBFO CryoProbe and a SampleCase automation device, to be housed in the NMR Facility at the Barnett Institute of Northeastern University. This versatile configuration will transform the capabilities of the two aging and oversubscribed instruments in our NMR core, a 500 MHz Varian INOVA and a 400 MHz Varian Mercury Plus, to meet rapidly increasing demands for chemical and biological structural analysis. The cutting-edge CryoProbe and automation capabilities of the requested 600 MHz NMR will enable a range of innovative projects at the forefront of interdisciplinary biomedical research, leaving routine usage to the existing NMR instruments. The following NIH-funded projects of the major users of the instrument will benefit immediately from the new features of the instrument: Kinetic target-guided synthesis to modulate protein-protein interactions (R. Manetsch); Discovery of lead and tool compounds for probing and modulating protozoan parasite biology (M. Pollastri/L. Ferrins); de novo synthesis of biologically active natural products and carbohydrate motifs (G.A. O’Doherty); Targeting the endocannabinoid system in treating brain disorders (A. Makriyannis); Profiling secondary metabolites in cell extracts of uncultured bacteria (K. Lewis); Improving oligonucleotide biopharmaceuticals by steric protection (K. Zhang); Dynamics of processivity clamp proteins in bacterial DNA replication (P. Beuning); and Transport of biologically active molecules across tight epithelial barriers (W. Lencer, Harvard Medical School).