600MHz NMR Spectrometer

项目摘要 我们建议购买Bruker Avance Neo 2通道600 MHz NMR光谱仪， BBFO CryoProbe和SampleCase自动化设备，将被安置在NMR设施中， 东北大学的巴内特研究所。这种多功能配置将改变 在我们的NMR核心，一个500 MHz的两个老化和超额认购的仪器的能力 Varian INOVA和400 MHz Varian Mercury Plus，以满足快速增长的 化学和生物结构分析。 所要求的600 MHz NMR的尖端CryoProbe和自动化功能将 使一系列创新项目处于跨学科生物医学研究的前沿， 留下常规使用现有的NMR仪器。以下NIH资助的项目 该仪器的主要用户将立即受益于 仪器：调节蛋白质-蛋白质相互作用的动力学靶向合成（R. 用于探测和调节原生动物的铅和工具化合物的发现 寄生生物学（M.波拉斯特里湖铁蛋白）;从头合成生物活性天然 产物和碳水化合物基序（G.A. O 'Doherty）;靶向内源性大麻素系统， 治疗脑部疾病（A. Makriyannis）;分析细胞提取物中的次级代谢产物 未培养细菌（K.刘易斯）;通过空间位阻改进寡核苷酸生物药物 保护（K. Zhang）;细菌DNA复制中持续合成能力钳蛋白的动力学（P. Beuning）;和生物活性分子穿过紧密上皮屏障的运输（W. Lencer，哈佛医学院）。

Project Summary We propose to acquire a Bruker Avance Neo 2-channel 600 MHz NMR spectrometer with a BBFO CryoProbe and a SampleCase automation device, to be housed in the NMR Facility at the Barnett Institute of Northeastern University. This versatile configuration will transform the capabilities of the two aging and oversubscribed instruments in our NMR core, a 500 MHz Varian INOVA and a 400 MHz Varian Mercury Plus, to meet rapidly increasing demands for chemical and biological structural analysis. The cutting-edge CryoProbe and automation capabilities of the requested 600 MHz NMR will enable a range of innovative projects at the forefront of interdisciplinary biomedical research, leaving routine usage to the existing NMR instruments. The following NIH-funded projects of the major users of the instrument will benefit immediately from the new features of the instrument: Kinetic target-guided synthesis to modulate protein-protein interactions (R. Manetsch); Discovery of lead and tool compounds for probing and modulating protozoan parasite biology (M. Pollastri/L. Ferrins); de novo synthesis of biologically active natural products and carbohydrate motifs (G.A. O’Doherty); Targeting the endocannabinoid system in treating brain disorders (A. Makriyannis); Profiling secondary metabolites in cell extracts of uncultured bacteria (K. Lewis); Improving oligonucleotide biopharmaceuticals by steric protection (K. Zhang); Dynamics of processivity clamp proteins in bacterial DNA replication (P. Beuning); and Transport of biologically active molecules across tight epithelial barriers (W. Lencer, Harvard Medical School).