Goodnight mouse: sleep and sepsis

摘要： 睡眠在哺乳动物生理学中起着至关重要的作用。充足的睡眠促进健康的免疫反应， 这有助于身体抵抗感染。在医院环境中，病人的睡眠经常因为夜间的原因而中断 护理、噪音和人造光。败血症是美国医院的主要死亡原因。这种情况 当感染导致免疫反应失调，最终导致器官功能障碍时，就会发生这种疾病。 尽管有现代医学治疗，严重脓毒症的死亡率仍然很高(30%-50%)。 先前的报告表明，睡眠中断会加剧小鼠的脓毒症，增加动物死亡率。然而， 这些效应背后的机制还没有被阐明。这项提议将证实睡眠 阻断会加重小鼠的脓毒症，并确定这种影响的机制。所获得的知识 这将为未来研究脓毒症和人类睡眠之间的交集奠定基础。 最终，败血症患者可能会受益于改善他们的睡眠数量和质量的干预措施，或者 药物干预以逆转睡眠不良的影响。 此外，这项提案还将为注册理科硕士课程的学生提供培训机会。 埃尔帕索生物医学研究生院的TTUHSC。埃尔帕索的人口80%是西班牙裔， 我们的硕士课程招收的少数族裔学生比例很高，包括少数族裔女性。 通过参与这个项目，我们的学生将获得重要的研究经验，获得体内和 体外研究技术、数据分析和统计分析，并将有机会介绍他们的 在一次全国会议上进行的研究。这些活动将极大地增加他们的职业机会和 加强埃尔帕索TTHSC的研究和教育基础设施。

Abstract: Sleep plays a vital role in mammalian physiology. Adequate sleep promotes a healthy immune response, which helps the body fight infections. In the hospital setting, patient sleep is often interrupted due to nighttime nursing care, noise, and artificial light. Sepsis is the leading cause of death in US hospitals. This condition develops when an infection induces a dysregulated immune response, culminating in organ dysfunction. Despite modern medical treatments, the mortality rate for severe sepsis remains very high (30-50%). Prior reports suggest that sleep interruption exacerbates sepsis in mice, increasing animal mortality. However, the mechanisms behind these effects have not been elucidated. This proposal will confirm that sleep interruption exacerbates sepsis in mice, and determine the mechanism of this effect. The knowledge gained will create a foundation for future studies that investigate the intersection between sepsis and sleep in humans. Ultimately, septic patients may benefit from interventions to improve their sleep quantity and quality, or pharmacological interventions to reverse the effects of poor sleep. Additionally, this proposal will provide training opportunities for students enrolled in the MSc program at TTUHSC at El Paso Graduate School of Biomedical Sciences. The population of El Paso is 80% Hispanic and our MSc program enrolls a high percentage of under-represented minority students, including minority women. By participating in this project, our students will gain critical research experience, acquiring skills for in vivo and in vitro research techniques, data analysis and statistical analysis, and will gain the opportunity to present their research at a national conference. These activities will greatly enhance their career opportunities and strengthen the infrastructure of research and education at TTHSC at El Paso.