Goodnight mouse: sleep and sepsis

晚安老鼠：睡眠和败血症

• 批准号: 10438217
• 负责人: Wendilywn Elizabeth Walker
• 金额: $ 13.47万
• 依托单位: TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER AT EL PASO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-06 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10438217
• 关键词: Acute; Admission activity; Adopted; Affect; Agonist; Antibiotics; Bacteria; Border Community; Care given by nurses; Caring; Cause of Death; Clinical; Control Groups; Critical Care; Data; Data Analyses; Disease; Doctor of Philosophy; Education; Electroencephalogram; Electromyography; Enrollment; Environment; Exhibits; FDA approved; Female; First Generation College Students; Foundations; Functional disorder; Future; Hispanic; Hispanic-serving Institution; Hospitals; Human; Immune response; In Vitro; Infection; Inflammation Mediators; Inflammatory; Inflammatory Response; Intensive Care Units; Interruption; Intervention; Knowledge; Light; Link; Liquid substance; Measurement; Measures; Medical; Middle Insomnia; Minority Women; Modeling; Modernization; Mus; Noise; Occupations; Opioid Analgesics; Organ; Outcome; Patients; Peritoneal Macrophages; Persons; Pharmacology; Physiology; Play; Population; Procedures; Production; Protocols documentation; Quality of life; Receptor Signaling; Recovery; Reporter; Reporting; Research; Research Assistant; Research Infrastructure; Research Technics; Research Training; Role; Schools; Science; Sepsis; Serum; Sleep; Sleep disturbances; Statistical Data Interpretation; Stress; Students; Survivors; TLR2 gene; Testing; Toll-like receptors; Underrepresented Minority; Work; adequate sleep; animal mortality; career; cecal ligation puncture; clinically relevant; crystalloid; cytokine; experience; fighting; graduate student; improved; in vivo; innovation; macrophage; male; medical schools; minority student; mortality; novel; poor sleep; programs; response; septic patients; sex; skills; sleep quality; sleep quantity; symposium; training opportunity

Abstract: Sleep plays a vital role in mammalian physiology. Adequate sleep promotes a healthy immune response, which helps the body fight infections. In the hospital setting, patient sleep is often interrupted due to nighttime nursing care, noise, and artificial light. Sepsis is the leading cause of death in US hospitals. This condition develops when an infection induces a dysregulated immune response, culminating in organ dysfunction. Despite modern medical treatments, the mortality rate for severe sepsis remains very high (30-50%). Prior reports suggest that sleep interruption exacerbates sepsis in mice, increasing animal mortality. However, the mechanisms behind these effects have not been elucidated. This proposal will confirm that sleep interruption exacerbates sepsis in mice, and determine the mechanism of this effect. The knowledge gained will create a foundation for future studies that investigate the intersection between sepsis and sleep in humans. Ultimately, septic patients may benefit from interventions to improve their sleep quantity and quality, or pharmacological interventions to reverse the effects of poor sleep. Additionally, this proposal will provide training opportunities for students enrolled in the MSc program at TTUHSC at El Paso Graduate School of Biomedical Sciences. The population of El Paso is 80% Hispanic and our MSc program enrolls a high percentage of under-represented minority students, including minority women. By participating in this project, our students will gain critical research experience, acquiring skills for in vivo and in vitro research techniques, data analysis and statistical analysis, and will gain the opportunity to present their research at a national conference. These activities will greatly enhance their career opportunities and strengthen the infrastructure of research and education at TTHSC at El Paso.

摘要： 睡眠在哺乳动物的生理机能中起着至关重要的作用。充足的睡眠可以促进健康的免疫反应， 帮助身体抵抗感染在医院环境中，患者睡眠经常由于夜间睡眠而中断。 护理、噪音和人工照明。败血症是美国医院的主要死亡原因。这种情况 当感染引起失调的免疫反应时，最终导致器官功能障碍。 尽管有现代医学治疗，严重脓毒症的死亡率仍然很高（30-50%）。 先前的报告表明，睡眠中断会加重小鼠的败血症，增加动物死亡率。然而，在这方面， 这些影响背后的机制尚未阐明。这一建议将证实睡眠 中断加重小鼠败血症，并确定这种作用的机制。获得的知识 将为未来研究脓毒症和人类睡眠之间的交叉点奠定基础。 最终，脓毒症患者可能会受益于改善睡眠数量和质量的干预措施， 药物干预，以扭转睡眠不佳的影响。 此外，该提案将为就读于硕士课程的学生提供培训机会， 埃尔帕索生物医学科学研究生院TTUHSC。埃尔帕索的人口80%是西班牙裔， 我们的理学硕士课程招收了高比例的代表性不足的少数民族学生，包括少数民族妇女。 通过参与这个项目，我们的学生将获得关键的研究经验，获得在体内的技能， 体外研究技术，数据分析和统计分析，并将有机会介绍他们的 在一次全国性会议上。这些活动将大大增加他们的职业机会， 加强埃尔帕索TTHSC的研究和教育基础设施。