Core B: Molecular Biology, Protein Expression and Structural Biology
Core B: Molecular Biology, Protein Expression and Structural Biology
批准号:
10439607
负责人:
Sriram Krishnaswamy
金额:
$65.52万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-09-01 至 2024-06-30
关键词:
AcidsAffinityAreaAwarenessBiologyCell LineChemistryChromatographyCoagulation ProcessCollaborationsCollectionContractsCore FacilityCrystallizationDataData AnalysesData CollectionDeuteriumDevelopmentDockingEnsureFactor VFactor VIIIFeasibility StudiesGoalsHemostatic functionHomeHuman ResourcesHydrogenIndividualLaboratoriesMammalian CellMeasurementMeasuresModelingMolecularMolecular BiologyMotivationPhysiologyPlasma ProteinsPost-Translational Protein ProcessingPreparationProductionPropertyProteinsProtocols documentationQuality ControlReactionReagentRecombinant ProteinsRecombinantsResearch PersonnelResolutionResourcesRoentgen RaysRunningScheduleSeriesSerumServicesSourceStructureSynchrotronsThrombosisTrainingUniversitiesVariantVisitVitamin KWorkX ray diffraction analysisX-Ray CrystallographyYeastsbasedata modelingexperienceexpression vectorhigh end computerinstrumentinterestliquid chromatography mass spectrometrymembermodel buildingnovelparallel computerprogramsprotein expressionprotein purificationrepositoryskillsstable cell linestructural biologysuccess
中文摘要
核心B摘要
英文摘要
Core B Abstract
The molecular biology, protein expression and structural biology core is organized around the pooling of the
resources and expertise of the project leaders in the program. The conduct of this core is based on a model
that has been successfully implemented in the past 10 years and has been extremely productive. This
centralized resource provides service and assistance in molecular biology, bacterial/yeast expression and in
the preparation of stable mammalian cell lines over-expressing vitamin K-dependent proteins, factor V variants
and factor VIII variants. The core provides the facilities, hardware and assistance for large-scale protein
production runs with stable or transiently transfected mammalian cells in serum-free conditioned media. The
core will provide facilities for large scale isolation of recombinant proteins, the appropriate chromatography
strategies and will maintain the affinity columns necessary for this activity. The core will also provide some
quality control services particularly the quantitation of -carboxyglutamic acid content to verify the integrity of
expressed vitamin K-dependent proteins. Assistance and facilities will also be provided for the large-scale
isolation of coagulation proteins from plasma. Core B personnel will assist program members in implementing
sparse matrix crystal screens, liaise with the UPenn x-ray facility to allow the collection of diffraction data on
the home source and provide expertise for optimization of crystallization conditions with expanded screens.
The core supports high performance computers equipped with necessary programs for processing diffraction
data, model building by molecular replacement and refinement. The core will also provide support for small
angle x-ray scattering measurements on the Home source and data analysis. Hydrogen/deuterium exchange
studies will be performed in collaboration with Renhao Li and facilitated by a modest sub-contract to Emory
University. The Director will be responsible for the oversight and efficient conduct of these activities and for
ensuring that equitable service is provided to the projects
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Core A-Administrative Core
-
批准号:10175001
-
项目类别:
-
资助金额:$24.91万
-
财政年份:2018
-
负责人:Sriram Krishnaswamy
-
依托单位:
Hemostasis and Thrombosis: Chemistry, Biology and Physiology
-
批准号:9982398
-
项目类别:
-
资助金额:$206.71万
-
财政年份:2018
-
负责人:Sriram Krishnaswamy
-
依托单位:
Hemostasis and Thrombosis: Chemistry, Biology and Physiology
-
批准号:9769852
-
项目类别:
-
资助金额:$254.87万
-
财政年份:2018
-
负责人:Sriram Krishnaswamy
-
依托单位:
Hemostasis and Thrombosis: Chemistry, Biology and Physiology
-
批准号:10439604
-
项目类别:
-
资助金额:$163.71万
-
财政年份:2018
-
负责人:Sriram Krishnaswamy
-
依托单位:
Hemostasis and Thrombosis: Chemistry, Biology and Physiology
-
批准号:10175000
-
项目类别:
-
资助金额:$163.71万
-
财政年份:2018
-
负责人:Sriram Krishnaswamy
-
依托单位:
Core B: Molecular Biology, Protein Expression and Structural Biology
-
批准号:10175002
-
项目类别:
-
资助金额:$65.52万
-
财政年份:2018
-
负责人:Sriram Krishnaswamy
-
依托单位:
Core A-Administrative Core
-
批准号:10439605
-
项目类别:
-
资助金额:$24.91万
-
财政年份:2018
-
负责人:Sriram Krishnaswamy
-
依托单位:
2012 Hemostasis Gordon Research Conference and Gordon Research Seminar
-
批准号:8388646
-
项目类别:
-
资助金额:$1.95万
-
财政年份:2012
-
负责人:Sriram Krishnaswamy
-
依托单位:
Proteinase Allostery and the Regulation of Blood Coagulation
-
批准号:8463606
-
项目类别:
-
资助金额:$39.87万
-
财政年份:2011
-
负责人:Sriram Krishnaswamy
-
依托单位:
Proteinase Allostery and the Regulation of Blood Coagulation
-
批准号:8299448
-
项目类别:
-
资助金额:$41.88万
-
财政年份:2011
-
负责人:Sriram Krishnaswamy
-
依托单位:
Proteinase Allostery and the Regulation of Blood Coagulation
-
批准号:8155012
-
项目类别:
-
资助金额:$41.88万
-
财政年份:2011
-
负责人:Sriram Krishnaswamy
-
依托单位:
Proteinase Allostery and the Regulation of Blood Coagulation
-
批准号:8662305
-
项目类别:
-
资助金额:$41.04万
-
财政年份:2011
-
负责人:Sriram Krishnaswamy
-
依托单位:
LIFETIME IMAGING OF COAGULATION ENZYME COMPLEXES
-
批准号:8362580
-
项目类别:
-
资助金额:$0.39万
-
财政年份:2011
-
负责人:Sriram Krishnaswamy
-
依托单位:
Novel Technologies for Imaging Blood Coagulation Reactions
-
批准号:7814133
-
项目类别:
-
资助金额:$41.16万
-
财政年份:2009
-
负责人:Sriram Krishnaswamy
-
依托单位:
Administrative Core
-
批准号:7663369
-
项目类别:
-
资助金额:$14.76万
-
财政年份:2009
-
负责人:Sriram Krishnaswamy
-
依托单位:
Macromolecular Substrate Recognition in Blood Coagulation
-
批准号:7663365
-
项目类别:
-
资助金额:$49.03万
-
财政年份:2009
-
负责人:Sriram Krishnaswamy
-
依托单位:
Novel Technologies for Imaging Blood Coagulation Reactions
-
批准号:7939742
-
项目类别:
-
资助金额:$42.0万
-
财政年份:2009
-
负责人:Sriram Krishnaswamy
-
依托单位:
FASEB Summer Conference on Proteases in Hemostasis and Vascular Biology
-
批准号:7276519
-
项目类别:
-
资助金额:$0.5万
-
财政年份:2007
-
负责人:Sriram Krishnaswamy
-
依托单位:
INTERACTION OF PROTHROMBINASE W/ PRODUCT & SUBSTRATE
-
批准号:6976514
-
项目类别:
-
资助金额:$0.13万
-
财政年份:2004
-
负责人:Sriram Krishnaswamy
-
依托单位:
Macromolecular Substrate Recognition in Blood Coagulation
-
批准号:7000523
-
项目类别:
-
资助金额:$43.77万
-
财政年份:2004
-
负责人:Sriram Krishnaswamy
-
依托单位:
海外基金