The E2F1 Post-Translational Modification Code
E2F1 翻译后修饰代码
基本信息
- 批准号:10440288
- 负责人:
- 金额:$ 43.55万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-01 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:AcetylationActive SitesApoptosisArginineBindingBromodomainCDKN2A geneCell Fate ControlCell ProliferationCell physiologyCellsChIP-seqChromatinChromatin StructureCodeComplexCyclin D1DNADNA Binding DomainDNA DamageDNA RepairDataDefectDevelopmentDouble Strand Break RepairE2F transcription factorsEP300 geneEffectivenessEnvironmentEnzymesGene ExpressionGenesGenetic TranscriptionGenome StabilityGoalsGrowthHistone AcetylationHistone H3HumanHypersensitivityImpairmentIonizing radiationKineticsKnock-inKnock-in MouseLaboratoriesLysineMalignant NeoplasmsMethylationModelingModificationMolecularMonitorMusMutationNucleosomesPathway interactionsPhosphorylationPhosphotransferasesPlayPost-Translational Protein ProcessingPredispositionProteinsPublishingReaderRoleSMARCA4 geneSignal TransductionSiteSkin CarcinogenesisSystemTestingTherapeutic InterventionTopoisomeraseTumor Suppressor ProteinsUV inducedUV induced DNA damageUltraviolet Raysbasecancer cellchromatin remodelingendonucleaseexperimental studyhistone acetyltransferasein vivoinhibitormouse modelnovelnovel therapeuticsp300/CBP-Associated Factorpreventpromoterrecruitrepairedresponsetool developmenttumortumor growthtumorigenesis
项目摘要
PROJECT SUMMARY
The E2F1 transcription factor is deregulated in most cancers through disruptions in the p16INK4A-cyclin D-RB
tumor suppressor pathway but the role E2F1 plays in cancer is complex as it can either promote or inhibit
tumor development depending on the context. E2F1 can also have opposing activities at the molecular level by
activating or repressing transcription and at the cellular level by promoting proliferation or apoptosis. Moreover,
E2F1 can directly stimulate DNA repair independent of its role in transcription. A long-term goal of these
studies is to understand how the various functions of E2F1 are regulated in the cell and how each
activity contributes to the development or suppression of cancer. E2F1 is phosphorylated, acetylated,
and methylated in response to DNA damage but how these modifications alter the activities of E2F1 remains
poorly understood. This proposal is based on the hypothesis that post-translational modifications on
E2F1 are read by interacting proteins to regulate E2F1 localization and functions in DNA repair,
transcription and tumorigenesis. The TopBP1 protein specifically binds to E2F1 when it is phosphorylated
by the ATM/ATR kinases and this interaction results in the recruitment of E2F1 to sites of DNA damage. Aim 1
studies will use a novel knock-in mouse model (S29A) that blocks E2F1 phosphorylation to determine the
function of E2F1 and its associated proteins in dynamically modifying chromatin structure at sites of DNA
damage to facilitate repair. Preliminary data demonstrates that E2F1 acetylation creates a binding motif for the
bromodomains of the CBP and p300 acetyltransferases. A novel knock-in mouse model that blocks E2F1
acetylation will be used to study how the E2F1-CBP/p300 interaction regulates histone acetylation and
modifies chromatin structure at sites of DNA damage to facilitate repair. These knock-in mice will also be used
to examine the role of E2F1 acetylation in regulating transcription and suppressing tumor development (Aim 2).
The TDRD3 protein was identified as a potential reader of an E2F1 arginine methylation mark (R109me). The
role of TDRD3 in regulating E2F1-dependent functions in DNA repair and transcription will be studied and, if
warranted, a knock-in mouse model will be developed to establish the role of this modification in modulating
tumorigenesis. Together, these studies will reveal how the E2F1 post-translational modification code is read by
various chromatin-modifying activities to regulate both DNA repair and transcription. Determining how these
E2F1 post-translational modifications impact the development of cancer will reveal new opportunities to
modulate the activities of E2F1 to inhibit tumor growth and enhance the effectiveness of therapies.
项目摘要
E2 F1转录因子在大多数癌症中通过p16 INK 4A-细胞周期蛋白D-RB的破坏而失调
肿瘤抑制途径,但E2 F1在癌症中发挥的作用是复杂的,因为它可以促进或抑制
肿瘤的发展取决于环境。E2 F1也可以在分子水平上具有相反的活性,
通过促进增殖或凋亡在细胞水平激活或抑制转录。此外,委员会认为,
E2 F1可以直接刺激DNA修复,而不依赖于其在转录中的作用。这些长期目标
研究的目的是了解E2 F1的各种功能在细胞中是如何调节的,以及每个功能是如何调节的。
活动有助于癌症的发展或抑制。E2 F1被磷酸化,乙酰化,
但这些修饰如何改变E2 F1的活性仍有待研究。
不太了解。该提议基于以下假设:
E2 F1被相互作用的蛋白质读取,以调节E2 F1的定位和在DNA修复中的功能,
转录和肿瘤发生。TopBP 1蛋白在磷酸化时特异性结合E2 F1
这种相互作用导致E2 F1被募集到DNA损伤位点。要求1
研究将使用一种新的基因敲入小鼠模型(S29 A),该模型阻断E2 F1磷酸化,以确定E2 F1的表达。
E2 F1及其相关蛋白在DNA位点动态修饰染色质结构中的功能
损坏,便于维修。初步数据表明,E2 F1乙酰化产生了一个结合基序,
CBP和p300乙酰转移酶的溴结构域。阻断E2 F1的新型基因敲入小鼠模型
乙酰化将用于研究E2 F1-CBP/p300相互作用如何调节组蛋白乙酰化,
修饰DNA损伤部位的染色质结构以促进修复。这些基因敲入小鼠也将被用于
研究E2 F1乙酰化在调节转录和抑制肿瘤发展中的作用(目的2)。
TDRD 3蛋白被鉴定为E2 F1精氨酸甲基化标记(R109 me)的潜在阅读器。的
将研究TDRD 3在DNA修复和转录中调节E2 F1依赖性功能的作用,如果
因此,将开发一种敲入小鼠模型,以确定这种修饰在调节
肿瘤发生总之,这些研究将揭示E2 F1翻译后修饰代码是如何被阅读的
各种染色质修饰活性来调节DNA修复和转录。确定这些
E2 F1翻译后修饰影响癌症的发展将揭示新的机会,
调节E2 F1的活性以抑制肿瘤生长并增强治疗效果。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The E2F1 transcription factor and RB tumor suppressor moonlight as DNA repair factors.
- DOI:10.1080/15384101.2020.1801190
- 发表时间:2020-09
- 期刊:
- 影响因子:0
- 作者:Manickavinayaham S;Velez-Cruz R;Biswas AK;Chen J;Guo R;Johnson DG
- 通讯作者:Johnson DG
Direct Regulation of DNA Repair by E2F and RB in Mammals and Plants: Core Function or Convergent Evolution?
- DOI:10.3390/cancers13050934
- 发表时间:2021-02-24
- 期刊:
- 影响因子:5.2
- 作者:Manickavinayaham S;Dennehey BK;Johnson DG
- 通讯作者:Johnson DG
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David G. Johnson其他文献
Charged behaviour from neutral ligands: synthesis and properties of N-heterocyclic pseudo-amides.
中性配体的带电行为:N-杂环假酰胺的合成和性质。
- DOI:
10.1002/chem.201103319 - 发表时间:
2012 - 期刊:
- 影响因子:0
- 作者:
R. Thatcher;David G. Johnson;John M. Slattery;R. Douthwaite - 通讯作者:
R. Douthwaite
Wellbeing in policy analysis
政策分析中的福祉
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
Phillip H. Smith;Frederick W. Merchant;David G. Johnson;W. Fujimoto;Robert H. Williams - 通讯作者:
Robert H. Williams
Spectacle and Sacrifice: The Ritual Foundations of Village Life in North China
- DOI:
10.2307/j.ctt1x07vxp - 发表时间:
2010-03 - 期刊:
- 影响因子:0
- 作者:
David G. Johnson - 通讯作者:
David G. Johnson
Observations of downwelling far-infrared emission at Table Mountain California made by the FIRST instrument
FIRST 仪器对加利福尼亚州桌山下流远红外发射的观测
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
M. Mlynczak;R. Cageao;J. Mast;D. Kratz;H. Latvakoski;David G. Johnson - 通讯作者:
David G. Johnson
Stratospheric age spectra derived from observations of water vapor and methane
来自水蒸气和甲烷观测的平流层年龄光谱
- DOI:
10.1029/1999jd900363 - 发表时间:
1999 - 期刊:
- 影响因子:0
- 作者:
David G. Johnson;K. Jucks;W. Traub;K. Chance;G. Toon;J. Russell;M. McCormick - 通讯作者:
M. McCormick
David G. Johnson的其他文献
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{{ truncateString('David G. Johnson', 18)}}的其他基金
MD Anderson Science Park Summer Program in Cancer Research SPCR
MD 安德森科学园癌症研究 SPCR 暑期项目
- 批准号:
8910669 - 财政年份:2014
- 资助金额:
$ 43.55万 - 项目类别:
MD Anderson Science Park Summer Program in Cancer Research SPCR
MD 安德森科学园癌症研究 SPCR 暑期项目
- 批准号:
9788953 - 财政年份:2014
- 资助金额:
$ 43.55万 - 项目类别:
MD Anderson Science Park Summer Program in Cancer Research SPCR
MD 安德森科学园癌症研究 SPCR 暑期项目
- 批准号:
10017162 - 财政年份:2014
- 资助金额:
$ 43.55万 - 项目类别:
MD Anderson Science Park Summer Program in Cancer Research SPCR
MD 安德森科技园癌症研究 SPCR 暑期项目
- 批准号:
8739963 - 财政年份:2014
- 资助金额:
$ 43.55万 - 项目类别:
MD Anderson Science Park Summer Program in Cancer Research SPCR
MD 安德森科学园癌症研究 SPCR 暑期项目
- 批准号:
9323335 - 财政年份:2014
- 资助金额:
$ 43.55万 - 项目类别:
Humanized Mouse Models for the p53 R72P SNP
p53 R72P SNP 人源化小鼠模型
- 批准号:
7812646 - 财政年份:2009
- 资助金额:
$ 43.55万 - 项目类别:
Humanized Mouse Models for the p53 R72P SNP
p53 R72P SNP 人源化小鼠模型
- 批准号:
8391757 - 财政年份:2008
- 资助金额:
$ 43.55万 - 项目类别:
Humanized Mouse Models for the p53 R72P SNP
p53 R72P SNP 人源化小鼠模型
- 批准号:
7996051 - 财政年份:2008
- 资助金额:
$ 43.55万 - 项目类别:
Humanized Mouse Models for the p53 R72P SNP
p53 R72P SNP 人源化小鼠模型
- 批准号:
8207922 - 财政年份:2008
- 资助金额:
$ 43.55万 - 项目类别:
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