Environmental and Genetic Factors Contributing to the Development of the Early-Life Oral Microbiome and its Influence on Early Childhood Caries

环境和遗传因素促进生命早期口腔微生物群的发展及其对儿童早期龋齿的影响

• 批准号: 10441629
• 负责人: Freida Anne Blostein
• 金额: $ 1.59万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10441629
• 关键词: 16S ribosomal RNA sequencing; Affect; Age; Appalachian Region; Behavioral; Behavioral Genetics; Breast Feeding; Child; Communities; Data; Dental; Dental Epidemiology; Dental Hygiene; Dental Plaque; Dental caries; Development; Diagnostic; Diet; Dietary Practices; Dimensions; Disease; Epidemiology; Etiology; Expenditure; Faculty; Genetic; Genetic Predisposition to Disease; Genetic Risk; Genome; Genotype; Goals; Habits; Health; Healthcare; Human; Human Genetics; Human Microbiome; Institution; Intervention; Lead; Learning; Life; Life Cycle Stages; Longitudinal Studies; Measures; Methodology; Methods; Microbial Biofilms; Microbial Genetics; Minority Groups; Modification; Mouth Diseases; National Institute of Dental and Craniofacial Research; Network-based; Oral; Oral cavity; Oral health; Pattern; Periodontal Diseases; Play; Population; Precision Health; Prevention; Primary Dentition; Research; Research Personnel; Research Proposals; Research Training; Risk; Risk Factors; Role; Saliva; Salivary; Sampling; Shapes; Structure; Testing; Training; Work; aged; base; career; cohort; computerized data processing; data reduction; early childhood; early life exposure; epidemiologic data; health data; high dimensionality; human data; improved; lifetime risk; longitudinal analysis; member; microbial; microbial colonization; microbiome; microbiome composition; microbiota; microorganism; modifiable risk; multidimensional data; oral microbial community; oral microbiome; polygenic risk score; prevent; saliva sample; skills; social disadvantage; sociodemographics

Project Abstract Caries that occur in the primary dentition of children aged under 6 years, or early childhood caries (ECC), affect more than one-fifth of US children aged 2-5. ECC is a multifactorial disease: the oral microbiome, host behaviors and genetic factors influence caries risk. Preventing ECC can improve oral and systemic health and reduce healthcare burden across the life course. By better understanding the causal mechanisms of ECC we may be able to improve upon prevention and intervention efforts. We will use already collected human genotypes, salivary and dental plaque microbiome, and sociodemographic and behavioral data from children participating in the Center for Oral Health Research in Appalachia cohort 2 to: 1) compare data reduction methods for characterizing the developing salivary microbiome, 2) assess the relationships between early-life exposures, the early-life oral microbiome and ECC, and 3) test for effect modification of the oral microbiomes’ relationship with ECC by host genetic caries risk. The proposed study has the potential to identify environmental and microbial factors which encourage or discourage the development of a cariogenic oral microbiome and to identify human genetic subpopulations for whom interventions on the oral microbiome may be especially effective. With this research proposal and training plan the applicant will gain skills in longitudinal analysis of high-dimensional data, processing of microbial and human genetic data and gain expertise in the multifactorial etiology of dental caries. The proposed training will support the applicant’s long-term goal of becoming an independent researcher focusing on the interplay between microbial, behavioral and human genetic factors in the development of oral disease. This project is consistent with NIDCR 2030 goals of Precision Health, Autotherapies and Oral Health + Overall Health.

项目摘要 6岁以下儿童或幼儿期乳牙发生的龋齿 龋齿 (ECC) 影响超过五分之一的美国 2-5 岁儿童。 ECC 是一个多因素 疾病：口腔微生物群、宿主行为和遗传因素影响龋齿风险。 预防 ECC 可以改善口腔和全身健康，并减轻整个系统的医疗负担 生命历程。通过更好地了解 ECC 的因果机制，我们或许能够改进 预防和干预工作。我们将使用已经收集的人类基因型， 唾液和牙菌斑微生物组，以及社会人口统计和行为数据 参加阿巴拉契亚口腔健康研究中心队列 2 的儿童可以： 1) 比较用于表征发育中唾液微生物组的数据简化方法， 2）评估早期暴露、早期口腔微生物组和 ECC 和 3) 测试宿主遗传对口腔微生物组与 ECC 关系的影响 龋齿风险。 拟议的研究有可能确定环境和微生物因素 鼓励或阻止致龋口腔微生物组的发展并确定 对口腔微生物组进行干预可能有效的人类遗传亚群 特别有效。 通过此研究计划和培训计划，申请人将获得纵向分析技能 高维数据、微生物和人类遗传数据的处理并获得以下方面的专业知识 龋齿的病因是多因素的。拟议的培训将支持申请人 成为一名独立研究者的长期目标，专注于研究之间的相互作用 口腔疾病发展中的微生物、行为和人类遗传因素。 该项目符合 NIDCR 2030 精准健康、自我疗法和口腔目标 健康+整体健康。