Community Engagement Plans of the Resource for Native MS Guided Structural Biology

我们提出了一种天然质谱学指导结构生物学的资源。该资源将构建， 验证和传播蛋白质结构表征的综合工作流程：蛋白质、膜 蛋白质：脂类和蛋白质：对一系列细胞和生物过程至关重要的RNA复合体。这个 该资源的广泛使命是使生物医学研究人员能够使用本地MS工作流来定义m/z， 化学计量、异质性、连接性、拓扑学、构象多样性和相对亚基结合 具有高灵敏度和高吞吐量的蛋白质复合体的优势。这些信息将指导选择性地 集成更高分辨率的结构生物学工具。社区参与核心的总体目标是 该资源旨在确保尽可能广泛地访问优化的本机MS工具和工作流程 通过促进国家研究界的积极参与。我们将通过共享工作流来实现这一点 除促进资源中开发的技术的商业化外，还将开发新的技术和技术。按顺序 为了实现这一点，资源将征募并积极支持将受益的协作和服务项目 在任何给定时间来自资源中可用的专业知识和成熟技术。我们已经这么做了 确定了22个这样的项目，随着这些项目的发展，我们将积极寻求新的合作和服务 项目，与生物医学研究社区合作，使现有的和新兴的本地多发性硬化症 容易获得和广泛可用的技术。此外，该资源还将提高对 原生MS通过培训生物医学研究人员在研究社区内应用 这些新颖的方法提出了自己的研究问题。我们将采取一些方法，以实现 这一目标，包括与协作与服务和推动生物医学项目团队的定期会议，以及 美国质谱学学会年会上的年度短期课程， 资源，以及实践培训活动，以及关于数据分析的资料丰富的文件和视频。这个 资源将促进技术传播和开发资源的商业化投入 尖端的天然MS引导结构生物学技术和方法进入生物医学手中 研究人员。为了确保快速、可持续地传播已开发的技术，我们将 依靠我们的行业和第三方供应商合作，为早期采用者提供商业前 供其实验室使用的产品。我们将从一小部分Beta测试者开始，并逐步实现完全测试 由多家主要仪器制造商提供商业产品，提出不同类型的仪器 性能特点和不同的价格范围。除了上面详述的传播方法之外， 我们将采用传统的方法，包括在同行评议的期刊上发表文章，并在 会议、会议和座谈会。

We propose a Resource for Native Mass Spectrometry Guided Structural Biology. The Resource will build, validate, and disseminate integrated workflows for structural characterization of protein:protein, membrane protein:lipid, and protein:RNA complexes that are critical for an array of cellular and organismal processes. The broad mission of the Resource is to empower biomedical researchers to use native MS workflows to define m/z, stoichiometry, heterogeneity, connectivity, topology, conformational diversity, and relative subunit binding strengths of protein complexes with high sensitivity and throughput. This information will guide selective integration of higher resolution structural biology tools. The overall goal of the Community Engagement Core of the Resource is to ensure the broadest possible access to optimized native MS instrumentation and workflows by fostering the active engagement of the national research community. We will do this by sharing workflows and technology in addition to fostering commercialization of the technologies developed in the Resource. In order to achieve this the resource will enlist and actively support Collaboration and Service projects that will benefit from the expertise and mature technologies available in the Resource at any given time. We have already identified 22 such projects, and as these projects develop we will actively seek new collaboration and service projects, working with the biomedical research community to make existing and emerging native MS technologies readily accessible and broadly available. In addition, the resource will improve understanding of native MS guided structural biology within the research community by training biomedical researchers to apply these novel methods to their own research questions. We will take a number of approaches in order to achieve this goal, including regular meetings with the Collaborate & Service and Driving Biomedical Projects teams, an annual short course at the American Society of Mass Spectrometry conference, an annual short course at the Resource, as well as hands-on training activities, and informative documents and videos on data analysis. The Resource will promote technology dissemination and commercialization of the developed resources to put cutting-edge native MS guided structural biology technologies and methods into the hands of biomedical researchers. In order to ensure rapid, sustainable dissemination, of the developed technologies, we will draw upon on our industrial and third party vendor collaborations to provide early adopters with pre-commercial products for use in their labs. We will start with a small number of beta testers and progress towards full commercial product offerings by multiple major instrument manufacturers, proposing instruments with different performance characteristics and different price ranges. Alongside the dissemination approaches detailed above, we will pursue traditional approaches, including publishing in peer reviewed journals and presenting the work at conferences, meetings, and symposia.