Community Engagement Plans of the Resource for Native MS Guided Structural Biology

本地 MS 引导结构生物学资源的社区参与计划

• 批准号: 10192756
• 负责人: Arpad Somogyi
• 金额: $ 33.36万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10192756
• 关键词: Adopted; American; Automobile Driving; Binding; Biomedical Research; Cell physiology; Characteristics; Collaborations; Communication; Communities; Complex; Computer software; Data Analyses; Educational workshop; Ensure; Fostering; Goals; Hand; Heterogeneity; Human Resources; Industrialization; Journals; Lipids; Manufacturer Name; Mass Spectrum Analysis; Membrane Proteins; Methods; Mission; Molecular Conformation; National Institute of General Medical Sciences; Online Systems; Peer Review; Performance; Persons; Physiological Processes; Price; Procedures; Proteins; Proteomics; Publications; Publishing; RNA; Research; Research Personnel; Residencies; Resolution; Resources; Services; Site; Societies; Structure; Technology; Time; Training; Training Activity; Tube; Universities; Vendor; Visit; Work; base; commercialization; community engagement; experience; improved; instrument; instrumentation; investigator training; meetings; movie; novel; protein complex; software development; stoichiometry; structural biology; symposium; tool; web site

We propose a Resource for Native Mass Spectrometry Guided Structural Biology. The Resource will build, validate, and disseminate integrated workflows for structural characterization of protein:protein, membrane protein:lipid, and protein:RNA complexes that are critical for an array of cellular and organismal processes. The broad mission of the Resource is to empower biomedical researchers to use native MS workflows to define m/z, stoichiometry, heterogeneity, connectivity, topology, conformational diversity, and relative subunit binding strengths of protein complexes with high sensitivity and throughput. This information will guide selective integration of higher resolution structural biology tools. The overall goal of the Community Engagement Core of the Resource is to ensure the broadest possible access to optimized native MS instrumentation and workflows by fostering the active engagement of the national research community. We will do this by sharing workflows and technology in addition to fostering commercialization of the technologies developed in the Resource. In order to achieve this the resource will enlist and actively support Collaboration and Service projects that will benefit from the expertise and mature technologies available in the Resource at any given time. We have already identified 22 such projects, and as these projects develop we will actively seek new collaboration and service projects, working with the biomedical research community to make existing and emerging native MS technologies readily accessible and broadly available. In addition, the resource will improve understanding of native MS guided structural biology within the research community by training biomedical researchers to apply these novel methods to their own research questions. We will take a number of approaches in order to achieve this goal, including regular meetings with the Collaborate & Service and Driving Biomedical Projects teams, an annual short course at the American Society of Mass Spectrometry conference, an annual short course at the Resource, as well as hands-on training activities, and informative documents and videos on data analysis. The Resource will promote technology dissemination and commercialization of the developed resources to put cutting-edge native MS guided structural biology technologies and methods into the hands of biomedical researchers. In order to ensure rapid, sustainable dissemination, of the developed technologies, we will draw upon on our industrial and third party vendor collaborations to provide early adopters with pre-commercial products for use in their labs. We will start with a small number of beta testers and progress towards full commercial product offerings by multiple major instrument manufacturers, proposing instruments with different performance characteristics and different price ranges. Alongside the dissemination approaches detailed above, we will pursue traditional approaches, including publishing in peer reviewed journals and presenting the work at conferences, meetings, and symposia.

我们提出了一个资源为本地质谱指导的结构生物学。资源将建立， 验证和传播蛋白质结构表征的集成工作流程：蛋白质、膜 蛋白质：脂质和蛋白质：RNA复合物，它们对一系列细胞和有机体过程至关重要。的 该资源的广泛使命是使生物医学研究人员能够使用原生MS工作流程来定义m/z， 化学计量、异质性、连接性、拓扑结构、构象多样性和相对亚基结合 具有高灵敏度和高通量的蛋白质复合物的优势。这些信息将指导选择性的 整合更高分辨率的结构生物学工具。社区参与核心的总体目标是 该资源旨在确保尽可能广泛地访问优化的本机MS工具和工作流程 促进国家研究界的积极参与。我们将通过共享工作流程来做到这一点 除了促进在资源中开发的技术的商业化之外，为了 为实现这一目标，该资源将招募并积极支持协作和服务项目， 在任何特定时间，资源中可用的专业知识和成熟技术。我们已经 确定了22个此类项目，随着这些项目的发展，我们将积极寻求新的合作和服务 项目，与生物医学研究界合作，使现有的和新兴的本土MS 技术容易获得和广泛提供。此外，该资源将提高对 本机MS指导结构生物学在研究社区通过培训生物医学研究人员应用 这些新颖的方法适合他们自己的研究问题。我们将采取多种方法， 这一目标，包括与协作与服务和推动生物医学项目团队定期举行会议， 美国质谱学会会议的年度短期课程， 资源，以及实践培训活动，以及关于数据分析的信息性文件和视频。的 资源将促进技术传播和商业化的开发资源， 尖端的原生MS引导结构生物学技术和方法进入生物医学领域， 研究人员为了确保快速、可持续地传播已开发的技术，我们将 基于我们的工业和第三方供应商合作，为早期采用者提供预商业化 在他们的实验室里使用的产品。我们将从少量的beta测试人员开始，并逐步向完整的beta测试人员迈进。 多个主要仪器制造商的商业产品供应，提出具有不同 性能特点和不同的价格范围。除了上文详述的传播方法外， 我们将采用传统的方法，包括在同行评议的期刊上发表，并在 会议、座谈会。