High-end MALDI Time of Flight Mass Spectrometer for Bioanalysis

项目摘要 质谱（MS）是支持亚利桑那州生物学和生物医学相关研究的重要工具 州立大学。2018年，ASU将其质谱服务集中在一个核心设施中， 整个亚利桑那州立大学和周围的学术和生物技术实体的100多名研究人员。亚利桑那州立大学感到自豪的是 被美国新闻连续第六次评为创新第一，但要保持这一地位， 服务于ASU研究社区及其学生的需求，核心和支持设施需要满足 下一代尖端研究环境的要求。亚利桑那州立大学的MS核心设施集中在 位于生物设计研究所，生物和医学发明的先驱，专注于社会问题 例如，基于唾液的COVID-19测试，该测试在2010年10月期间向亚利桑那州公众提供， 持续的流行病。亚利桑那州立大学和生物设计研究所也蓬勃发展，作为生物分子的顶级研究机构 结构解析，以解开负责生物和医学过程的关键生物分子细节 在代谢和疾病如癌症和神经退行性疾病中的作用。亚利桑那州立大学的研究人员还努力 在获得生物分子的签名，重要的是了解能量转换和光合作用，以解决我们的 以及下一代的能源需求。此外，ASU分子科学学院在其 教师们有才华的年轻和成熟的研究人员，采取分子科学到一个新的水平， 创新的综合办法。所有这些领域都需要可靠和灵活的结构说明 工具，在许多情况下通过基质辅助激光解吸电离来完成或补充 然而，亚利桑那州立大学的MS核心设施只拥有一个相当基本的MALDI MS仪器，我们 我打算通过目前的建议，补充一个更敏感和更高分辨率的仪器，还 能够进行MALDI成像。有了这台新的MALDI MS仪器，我们将升级现有的MS分析 ASU的能力，以满足我们目前资助的NIH研究人员的需求，但也提供了一个工具， 通过高端质谱技术促进下一代研究项目的发展。

PROJECT SUMMARY Mass spectrometry (MS) is an essential tool to support biologically and biomedically related research at Arizona State University (ASU). In 2018, ASU has centralized its mass spectrometry services in a core facility that serves over 100 researchers throughout ASU and surrounding academic and biotechnology entities. ASU is proud of having been recognized as #1 in innovation for the 6th time in a row by US News, but to hold this status and serve the needs for the ASU research community and its students, core and support facilities need to meet the requirements of the next generation cutting-edge research environment. The MS Core Facility at ASU is centrally located in the Biodesign Institute, a forerunner of biological and medical invention focusing on societal problems such as for example a saliva based COVID-19 test that was made available to the Arizona public during the ongoing pandemic. ASU and the Biodesign Institute also thrive as a top research institution for biomolecular structure elucidation to unlock key biomolecular details responsible for biological and medical processes involved in metabolism and diseases such as cancer and neurodegenerative disorders. Researchers at ASU also strive in gaining biomolecular signatures important to understand energy conversion and photosynthesis to solve our and future generation’s energy needs. In addition, the School of Molecular Sciences at ASU has among its faculty members talented young and established researchers that take molecular sciences to the next level with innovative synthesis approaches. All these areas require reliable and flexibly accessible structure elucidation tools, which in many cases is accomplished or supplemented through matrix assisted laser desorption ionization (MALDI) MS. However, the MS core facility at ASU only owns one rather basic MALDI MS instrument, which we intend to supplement through the current proposal with a more sensitive and higher resolution instrument also capable of MALDI imaging. With this new MALDI MS instrument we will upgrade the available MS analysis capabilities at ASU to meet the needs of our currently funded NIH researchers but also provide a tool paving the way for the next generation of research projects facilitated through high end mass spectrometry.