High-end MALDI Time of Flight Mass Spectrometer for Bioanalysis

项目概要 质谱 (MS) 是支持亚利桑那州生物学和生物医学相关研究的重要工具 州立大学（ASU）。 2018 年，亚利桑那州立大学将其质谱服务集中在一个核心设施中，该设施为 亚利桑那州立大学及其周边学术和生物技术实体的 100 多名研究人员。亚利桑那州立大学感到自豪 连续 6 次被《美国新闻》评为创新领域第一名，但要保持这一地位， 满足亚利桑那州立大学研究界及其学生的需求，核心和支持设施需要满足 下一代尖端研究环境的要求。亚利桑那州立大学的 MS 核心设施集中在 位于生物设计研究所，生物和医学发明的先驱，专注于社会问题 例如，在疫情期间向亚利桑那州公众提供的基于唾液的 COVID-19 检测 持续的大流行。亚利桑那州立大学和生物设计研究所也作为生物分子领域的顶级研究机构蓬勃发展 结构阐明以解开负责所涉及的生物和医学过程的关键生物分子细节 新陈代谢和癌症和神经退行性疾病等疾病。亚利桑那州立大学的研究人员也致力于 获得生物分子特征对于理解能量转换和光合作用以解决我们的问题很重要 和下一代的能源需求。此外，亚利桑那州立大学分子科学学院还拥有 教职人员中有才华横溢的年轻和成熟的研究人员，他们将分子科学提升到一个新的水平 创新的合成方法。所有这些领域都需要可靠且灵活的结构阐明 工具，在许多情况下是通过基质辅助激光解吸电离来完成或补充的 （马尔迪）女士。然而，ASU 的 MS 核心设施只拥有一台相当基本的 MALDI MS 仪器，我们 还打算通过当前提案补充一个更灵敏、更高分辨率的仪器 能够进行MALDI成像。借助这款新型 MALDI MS 仪器，我们将升级可用的 MS 分析 亚利桑那州立大学的能力可以满足我们目前资助的 NIH 研究人员的需求，同时也提供了一个工具，为 通过高端质谱促进下一代研究项目的方式。

PROJECT SUMMARY Mass spectrometry (MS) is an essential tool to support biologically and biomedically related research at Arizona State University (ASU). In 2018, ASU has centralized its mass spectrometry services in a core facility that serves over 100 researchers throughout ASU and surrounding academic and biotechnology entities. ASU is proud of having been recognized as #1 in innovation for the 6th time in a row by US News, but to hold this status and serve the needs for the ASU research community and its students, core and support facilities need to meet the requirements of the next generation cutting-edge research environment. The MS Core Facility at ASU is centrally located in the Biodesign Institute, a forerunner of biological and medical invention focusing on societal problems such as for example a saliva based COVID-19 test that was made available to the Arizona public during the ongoing pandemic. ASU and the Biodesign Institute also thrive as a top research institution for biomolecular structure elucidation to unlock key biomolecular details responsible for biological and medical processes involved in metabolism and diseases such as cancer and neurodegenerative disorders. Researchers at ASU also strive in gaining biomolecular signatures important to understand energy conversion and photosynthesis to solve our and future generation’s energy needs. In addition, the School of Molecular Sciences at ASU has among its faculty members talented young and established researchers that take molecular sciences to the next level with innovative synthesis approaches. All these areas require reliable and flexibly accessible structure elucidation tools, which in many cases is accomplished or supplemented through matrix assisted laser desorption ionization (MALDI) MS. However, the MS core facility at ASU only owns one rather basic MALDI MS instrument, which we intend to supplement through the current proposal with a more sensitive and higher resolution instrument also capable of MALDI imaging. With this new MALDI MS instrument we will upgrade the available MS analysis capabilities at ASU to meet the needs of our currently funded NIH researchers but also provide a tool paving the way for the next generation of research projects facilitated through high end mass spectrometry.