The Bioimaging Core

总结 哥伦比亚大学消化和肝病研究中心（CU-）的生物成像核心（BIC） DLDRC）将为消化系统疾病的理解和治疗做出重要贡献。国家 最先进的成像技术可以解决细胞、器官、代谢物和微生物群之间的复杂相互作用， 调节消化系统的健康和疾病。然而，维护和操作复杂系统的成本和专业知识 成像平台超出了大多数单个实验室的财政和技术手段。总目标 BIC的目的是为CU-DLDRC成员提供最先进的成像平台，以研究 消化健康和疾病的小动物和人体生物标本在体外和体内，提供解决方案 范围从纳米到厘米。BIC分为两个单元，以高端消化为重点， 由专家技术援助支持的成像能力，每个都由一位高素质的主管领导， 在影像学和核心管理方面的长期经验。由BIC联合主任监督的显微镜部门 Liza Pon提供了一套先进的显微镜，包括单光子，多光子和旋转显微镜 光盘共聚焦，超分辨率和全内反射荧光显微镜，它允许快速和高， 活的或固定的消化组织标本的分辨率成像。小动物成像单位，由BIC监督 主任肯橄榄，提供访问高端磁共振成像，超声，X射线计算机 断层扫描和光学成像仪器，用于对小动物的消化器官及其功能进行成像。 此外，BIC将开发新的消化道成像模式，包括（i）成像质量 光谱与CU-DLDRC成员布伦特斯托克韦尔为高分辨率成像的代谢物在消化道 组织;和（ii）SCAPE成像，一种基于内源性组织荧光的新型中尺度光学平台， 由CU-DLDRC成员Elizabeth Hillman设计。小动物成像部门还将开发新的 专门用于评估胃肠道运动、纤维化、炎症、跟踪工程化 微生物和代谢物的体内测量。BIC将为CU的整体使命做出贡献- DLDRC通过以下相互关联的目的：分析细胞结构和过程，调节 通过显微镜观察消化组织的上皮健康（目标1）;提供体内小动物成像技术 研究消化生理学和疾病（目标2）;并提供实践培训和教育， 高级消化成像应用（目标3）。BIC将是一个高度利用的资源，49个CU中有43个- 荒漠化、土地退化和资源中心成员（88%）计划使用其服务。通过多核工作流，BIC将与其他 CU-DLDRC生物医学核心，从而为CU-DLDRC成员提供方便的成像临床 生物标本和类器官，或将其成像与先进的生物信息学分析相结合。总而言之，BIC 将有利于CU-DLDRC成员的基础和临床研究，并提供显著的附加值。

SUMMARY The Bioimaging Core (BIC) in the Columbia University Digestive and Liver Disease Research Center (CU- DLDRC) will make essential contributions for the understanding and treatment of digestive diseases. State-of- the-art imaging can resolve the complex interactions between cells, organs, metabolites and microbiota that regulate digestive health and disease. However, the cost and expertise of maintaining and operating complex imaging platforms are beyond the financial and technical means of most individual laboratories. The overall goal of the BIC is to provide CU-DLDRC members with state-of-the-art imaging platforms to study mechanisms of digestive health and disease in small animals and human biospecimens in vitro and in vivo, providing resolutions ranging from nanometers to centimeters. The BIC is organized into two units with high-end digestive-focused imaging capabilities backed by expert technical assistance, each led by a highly qualified director with longstanding experience in imaging and core administration. The Microscopy Unit, overseen by BIC Co-Director Liza Pon, provides access to a sophisticated suite of microscopes, including single, multiphoton and spinning disk confocal, super-resolution and total internal reflection fluorescence microscopy, which allow rapid and high- resolution imaging of living or fixed digestive tissue specimens. The Small Animal Imaging Unit, overseen by BIC director Ken Olive, provides access to high-end magnetic resonance imaging, ultrasound, X-ray computed tomography, and optical imaging instruments for imaging of digestive organs and their functions in small animals. In addition, the BIC will develop new digestive-focused imaging modalities, including (i) imaging mass spectrometry with CU-DLDRC member Brent Stockwell for high resolution imaging of metabolites in digestive tissue; and (ii) SCAPE imaging, a novel meso-scale optical platform based on endogenous tissue fluorescence, engineered by CU-DLDRC member Elizabeth Hillman. The Small Animal Imaging unit will also develop novel applications specific for the assessment of gastrointestinal motility, fibrosis, inflammation, tracking of engineered microbes, and in vivo measurement of metabolites. The BIC will contribute to the overall mission of the CU- DLDRC through the following interrelated Aims: To analyze cellular structures and processes that regulate epithelial health in digestive tissues via microscopy (Aim 1); to provide in vivo small animal imaging technologies for the study of digestive physiology and disease (Aim 2); and to provide hands-on training and education in advanced digestive imaging applications (Aim 3). BIC will be a highly utilized resource with 43 out of the 49 CU- DLDRC members (88%) planning to use its services. Via multicore workflows, BIC will closely link to the other CU-DLDRC biomedical cores, thus providing CU-DLDRC members with easy access to imaging clinical biospecimens and organoids, or combining their imaging with advanced bioinformatics analyses. In sum, BIC will benefit the research of basic and clinical CU-DLDRC members and provide significant value added.