The Bioimaging Core

摘要 哥伦比亚大学消化和肝病研究中心的生物成像核心(BIC) DLDRC)将为了解和治疗消化系统疾病做出重要贡献。现状-- -ART成像可以解决细胞、器官、代谢物和微生物群之间的复杂相互作用 调节消化健康和疾病。然而，维护和运营综合体的成本和专业知识 成像平台超出了大多数单独实验室的财务和技术手段。总目标 BIC的目的是为CU-DLDRC成员提供最先进的成像平台，以研究其机制 小动物的消化健康和疾病以及体外和体内的人类生物样本，提供解决方案 从纳米到厘米不等。BIC由两个单位组成，以高端消化为重点 由专家技术援助支持的成像能力，每个能力都由一名高资质的主管领导， 在成像和核心管理方面有长期经验。显微镜部门，由BIC联席主任监督 Liza Pon提供了一套复杂的显微镜，包括单光子、多光子和自旋显微镜 圆盘共聚焦、超分辨率和全内反射荧光显微镜，允许快速和高分辨率地 活的或固定的消化组织标本的分辨率成像。由BIC监督的小动物成像单位 导演Ken Olive，提供高端磁共振成像、超声波、X光计算机 用于小动物消化器官及其功能成像的断层摄影术和光学成像仪。 此外，BIC将开发新的以消化为重点的成像方式，包括(I)成像质量 CU-DLDRC成员布伦特·斯托克韦尔高分辨率成像消化系统代谢物 和(Ii)SCAPE成像，这是一种基于组织内源荧光的新型中尺度光学平台， 由CU-DLDRC成员伊丽莎白·希尔曼设计。小动物成像单位也将开发新的 专门用于评估胃肠动力、纤维化、炎症、跟踪工程化 微生物和体内代谢物的测量。BIC将为CU的总体使命做出贡献- DLDRC通过以下相互关联的目标：分析细胞结构和调节 通过显微镜观察消化组织上皮的健康(目标1)；提供活体小动物成像技术 研究消化生理学和疾病(目标2)；并在以下方面提供实践培训和教育 高级消化成像应用(目标3)。BIC将是一种利用率很高的资源，49个CU中有43个 DLDRC成员(88%)计划使用其服务。通过多核工作流，BIC将与另一个紧密相连 CU-DLDRC生物医学核心，从而为CU-DLDRC成员提供轻松访问成像临床的途径 生物有机体和有机物，或将其成像与先进的生物信息学分析相结合。总而言之，BIC 将有助于CU-DLDRC成员的基础和临床研究，并提供显著的附加值。

SUMMARY The Bioimaging Core (BIC) in the Columbia University Digestive and Liver Disease Research Center (CU- DLDRC) will make essential contributions for the understanding and treatment of digestive diseases. State-of- the-art imaging can resolve the complex interactions between cells, organs, metabolites and microbiota that regulate digestive health and disease. However, the cost and expertise of maintaining and operating complex imaging platforms are beyond the financial and technical means of most individual laboratories. The overall goal of the BIC is to provide CU-DLDRC members with state-of-the-art imaging platforms to study mechanisms of digestive health and disease in small animals and human biospecimens in vitro and in vivo, providing resolutions ranging from nanometers to centimeters. The BIC is organized into two units with high-end digestive-focused imaging capabilities backed by expert technical assistance, each led by a highly qualified director with longstanding experience in imaging and core administration. The Microscopy Unit, overseen by BIC Co-Director Liza Pon, provides access to a sophisticated suite of microscopes, including single, multiphoton and spinning disk confocal, super-resolution and total internal reflection fluorescence microscopy, which allow rapid and high- resolution imaging of living or fixed digestive tissue specimens. The Small Animal Imaging Unit, overseen by BIC director Ken Olive, provides access to high-end magnetic resonance imaging, ultrasound, X-ray computed tomography, and optical imaging instruments for imaging of digestive organs and their functions in small animals. In addition, the BIC will develop new digestive-focused imaging modalities, including (i) imaging mass spectrometry with CU-DLDRC member Brent Stockwell for high resolution imaging of metabolites in digestive tissue; and (ii) SCAPE imaging, a novel meso-scale optical platform based on endogenous tissue fluorescence, engineered by CU-DLDRC member Elizabeth Hillman. The Small Animal Imaging unit will also develop novel applications specific for the assessment of gastrointestinal motility, fibrosis, inflammation, tracking of engineered microbes, and in vivo measurement of metabolites. The BIC will contribute to the overall mission of the CU- DLDRC through the following interrelated Aims: To analyze cellular structures and processes that regulate epithelial health in digestive tissues via microscopy (Aim 1); to provide in vivo small animal imaging technologies for the study of digestive physiology and disease (Aim 2); and to provide hands-on training and education in advanced digestive imaging applications (Aim 3). BIC will be a highly utilized resource with 43 out of the 49 CU- DLDRC members (88%) planning to use its services. Via multicore workflows, BIC will closely link to the other CU-DLDRC biomedical cores, thus providing CU-DLDRC members with easy access to imaging clinical biospecimens and organoids, or combining their imaging with advanced bioinformatics analyses. In sum, BIC will benefit the research of basic and clinical CU-DLDRC members and provide significant value added.