Sleep Based Memory Consolidation in Juvenile Zebra Finch

项目摘要 睡眠被认为在巩固多种形式的记忆方面起着重要作用 发展。短暂的睡眠中断往往会导致习得的熟练行为的延迟和丧失。而当 有关睡眠在大鼠海马区依赖学习中的作用的研究取得了很大进展 外显记忆，睡眠如何影响与学习知觉和程序有关的突触电路 人们对记忆仍然知之甚少。鸣禽为研究睡眠提供了无与伦比的机会 巩固熟练的运动行为。幼年鸣鸟通过每天广泛的学习它们的求爱歌曲 练习，在成年前制作数以万计的演唱。每天，听觉反馈引导的改进 假设在睡眠期间每晚都会巩固歌声，使新的声音探索和 通过发展不断完善歌曲表演。我的博士前研究试图找出 突触回路参与基于睡眠的SONG巩固和短暂性干扰的长期影响 在歌曲发展过程中的这些回路中。通过将体内药理操作与机器相结合 基于学习的方法来分离和分析歌唱行为的变化，我至少确定了 一个核团支配着基于睡眠的复杂运动行为的巩固。有待完成的剩余工作 在F99阶段，将重点介绍单个运动前突触回路的具体贡献 突触特异性药理学与通路特异性化学遗传学的结合。同样在F99阶段， 我将跟进意想不到的发现，以确定歌曲多样性在配偶选择中的进化相关性。 该计划的K00阶段将提供一个具体的计划，为以下项目寻找理想的导师和研究环境 增强我在神经入侵和病毒繁殖方面的知识和技能 用于有效解剖神经回路的新型病毒载体。总而言之，研究和培训详细说明 这项提议的两个阶段将使我能够实现我的长期目标，即设计复杂的工具 了解作为终身教职学习的复杂运动行为的分子、细胞和突触基础 首席调查员。

Project Summary Sleep is thought to play a significant contribution in the consolidation of multiple forms of memories during development. Transient disruptions of sleep often lead to delays and losses in learned skilled behaviors. While research has made great progress in understanding the role of sleep in the hippocampal dependent learning of explicit memories, how sleep influences the synaptic circuits involved in learning perceptual and procedural memories is still poorly understood. Songbirds provide an unmatched opportunity to study sleep based consolidation of skilled motor behaviors. Juvenile songbirds learn their courtship song through extensive daily practice, producing tens of thousands of renditions by adulthood. Daily, auditory-feedback guided improvements in song are hypothesized to be consolidated nightly during sleep, enabling renewed vocal exploration and continued refinement of song performances through development. My predoctoral research seeks to identify the synaptic circuits involved in sleep-based consolidation of song and the long-term impact of transient disruptions in these circuits during song development. By combining in vivo pharmacological manipulations with machine learning-based approaches for segregating and analyzing changes in singing behavior, I have identified at least one nuclei governing the sleep-based consolidation of complex motor behaviors. Remaining work to be done during the F99 phase will focus on the specific contributions of individual premotor synaptic circuits using synapse-specific pharmacology in conjunction with pathway specific chemogenetics. Also during the F99 phase, I will follow up on unexpected findings to determine the evolutionary relevance of song diversity in mate choice. The K00 phase of this proposal will provide a specific plan to find an ideal mentor and research environment for enhancing my knowledge and technical skills of neuroinvasion and propagation of viruses leading to generation of novel viral vectors for the effective dissection of neural circuits. Together, the research and training detailed in the two phases of this proposal will allow me to achieve my long-term goal of engineering sophisticated tools to understand the molecular, cellular, and synaptic basis of learned complex motor behaviors as a tenured principal investigator.