Core C: Customized Physiology and Imaging Core

项目摘要 拟议的佛蒙特心血管和心脏病中心的新定制成像和生理学核心 脑健康COBRE将基于神经科学成像核心的转变， COBRE（2016年完成）。它将满足尖端成像调查的新需求， 在新提议的COBRE中，项目主任（PD）将继续努力。我们将收购所需的 技术和改造，以适应其运作。定制的生理和成像核心将 旨在为活体样本（包括活体、组织和细胞）提供最先进的成像能力 制备物以及固定样品。新的心血管和大脑健康COBRE将涉及 在体内成像和电生理学方面的广泛培训，以及遗传编码指标的开发 以及项目主管的光学驱动探头。该核心将具有高水平的实验前和实验后 技术和分析支持来自托德克拉森，格兰特亨尼格博士和汤姆赫普纳博士。新时代 高分辨率显微镜需要在清醒的动物中成像的灵活性， 任务，并在从毫秒到数周的广泛时间段内以三维方式成像。到 为了实现这一目标，该核心将建议和培训调查人员使用不同类型的最先进的高 空间和时间分辨率显微镜、电生理技术和仪器3D打印 设计和制造，以应对不断变化的研究需求，以及在使用和发展的形象 和电生理分析软件。该核心的目标是使调查人员具备以下能力： 成像/电生理，制造和分析技能，使他们能够适应的方法， 解决复杂问题。预计这一核心将大大提高COBRE的竞争力 心血管和大脑健康研究的项目总监以及校园范围内的研究人员。这一核心 独特之处在于，PD和其他调查人员在高度成就的核心人员的支持下，将 获得执行实验的技能，修改成像模式，制造特定项目 设备，并进行高水平的图像分析。

PROJECT SUMMARY The new Customized Imaging and Physiology Core of the proposed Vermont Center for Cardiovascular and Brain Health COBRE will be based upon transformation of a Neuroscience Imaging Core from a previous COBRE (completed in 2016). It will meet the new needs of cutting-edge imaging investigation that will be pursued by Project Directors (PDs) in the newly proposed COBRE. We will employ acquisition of needed technology and renovation to accommodate its operation. The Customized Physiology and Imaging Core will be designed to provide state-of-the-art imaging capabilities for live samples, including intravital, tissue and cell preparations, as well as fixed samples. The new Cardiovascular and Brain Health COBRE will involve extensive training in in vivo imaging and electrophysiology, and exploitation of genetically-encoded indicators and optically-actuated probes by Project Directors. This Core will have high level pre- and post-experiment technical and analytical support from Todd Clason, Dr. Grant Hennig and Dr. Tom Heppner. The new era of high-resolution microscopy requires the flexibility to image in awake animals that may be undergoing specific tasks, and to image in 3 dimensions over a wide range of time periods from milliseconds to weeks. To accomplish this, this Core will advise and train investigators in the use of different types of state-of-art high spatial and temporal resolution microscopy, electrophysiological techniques, and 3D printing for instrument design and fabrication to respond to changing research needs, as well as in use and development of image and electrophysiological analysis software. The goal of this Core is to equip the investigators with imaging/electrophysiological, fabrication and analysis skills so that they are capable of adapting approaches to solve complex problems. It is anticipated that this Core will greatly enhance the competitiveness of COBRE Project Directors as well as campus-wide investigators in cardiovascular and brain health research. This Core is unique in that PDs and other investigators, with support from highly accomplished Core personnel, will acquire the skills to perform the experiments, modify imaging modalities, to fabricate project-specific equipment, and to perform high level image analysis.