3D-Printed Demineralized Bone Matrix Hydrogels for Craniofacial Bone Tissue Regeneration.

3D 打印脱矿骨基质水凝胶用于颅面骨组织再生。

• 批准号: 10451625
• 负责人: Katie Hogan
• 金额: $ 4.76万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-09 至 2023-09-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10451625
• 关键词: 3D Print; Alkaline Phosphatase; Architecture; Autologous Transplantation; Biochemical; Biocompatible Materials; Blood Vessels; Bone Matrix; Bone Regeneration; Bone Resorption; Bone Tissue; Calcium; Cells; Cleft Palate; Clinical; Complex; Congenital Abnormality; Cues; Dental; Dental Implantation; Devices; Disease; Ensure; Excision; Extracellular Matrix; Geometry; Goals; Growth Factor; Height; Histology; Hydrogels; Implant; In Vitro; Infection; Kinetics; Lead; Measures; Membrane; Mesenchymal Differentiation; Mesenchymal Stem Cells; Modeling; Morbidity - disease rate; Natural regeneration; Operative Surgical Procedures; Parietal bone structure; Patients; Periodontal Diseases; Porosity; Procedures; Property; Rattus; Research; Research Project Grants; Risk; Role; Site; Swelling; Testing; Time; Tissue Engineering; Tissues; Tooth Diseases; Tooth Extraction; Translations; Trauma; Work; alveolar bone; bone; bone quality; clinically relevant; craniofacial; craniofacial bone; craniofacial tissue; crosslink; demineralization; density; design; hydrogel scaffold; improved; in vivo; in vivo Model; in vivo regeneration; infection rate; insight; mechanical properties; microCT; nanoparticle; osteogenic; palate repair; reconstruction; scaffold; standard care; stem cell proliferation; tissue regeneration; tumor

Project Summary/Abstract There is a clinical need for craniofacial bone augmentation in cases of trauma, tumor resection, congenital malformations, or bone resorption as a result of tooth extraction or periodontal disease. Currently, bone autograft, which is associated with increased risk of donor site morbidity and infection, and non-resorbable barrier membranes, which required additional procedures for removal, are the standard of treatment. Therefore, the objective of this research is to develop 3D printed scaffolds for craniofacial bone augmentation using a clinically relevant material, demineralized bone matrix, for guided bone regeneration. The fundamental hypothesis for this research project is that the endogenous biochemical cues for bone regeneration found within demineralized bone matrix combined with the ability to tune scaffold microarchitecture and crosslinking via 3D printing will result in improved bone augmentation. Two specific aims will be investigated to accomplish the goals of this project. In the first specific aim, we will fabricate 3D-printed scaffolds composed of demineralized bone matrix nanoparticles with varied pore sizes and UV crosslinking times. The mechanical properties and degradation kinetics of these scaffolds will then be characterized via compressive testing, bulk swelling, and mass loss studies. Additionally, the in vitro osteogenic potential of the demineralized bone matrix scaffolds will be evaluated using mesenchymal stem cells and measured by alkaline phosphatase expression, calcium content, and markers of osteogenic differentiation. The results of this specific aim will elucidate the role of pore size and UV crosslinking in determining the osteogenic potential of demineralized bone matrix scaffolds and determine which groups are appropriate for in vivo translation. In the second specific aim, we will investigate the in vivo osteoinductive and osteoconductive capacity of 3D-printed demineralized bone matrix scaffolds. The scaffolds will be implanted in a rat parietal bone augmentation model for 12 weeks and assessed via micro-CT and histology for newly formed bone volume, maximum bone height, and bone quality. Upon completion of the proposed work, we will have determined the in vitro and in vivo bone regeneration efficacy of an acellular, 3D-printed demineralized bone matrix scaffold and demonstrated tunability of scaffold mechanical properties and degradation. Additionally, the proposed work provide new insights into rational 3D- printed scaffold design and fabrication for craniofacial applications.

项目总结/文摘

项目成果

Self-rectifying magnetoelectric metamaterials for remote neural stimulation and motor function restoration.

用于远程神经刺激和运动功能恢复的自整流磁电超材料。

• DOI: 10.1038/s41563-023-01680-4
• 发表时间: 2024
• 期刊: Nature materials
• 影响因子: 41.2
• 作者: Chen,JoshuaC;Bhave,Gauri;Alrashdan,Fatima;Dhuliyawalla,Abdeali;Hogan,KatieJ;Mikos,AntoniosG;Robinson,JacobT
• 通讯作者: Robinson,JacobT