Spatial and temporal control of Rho family GTPases

许多基本的细胞功能需要大规模的细胞皮质组织。 这些包括胞质分裂、不对称细胞分裂、细胞迁移和形成 上皮组织。细胞骨架和细胞-细胞连接的广泛组织是 在发育过程中所需的，以推动细胞、胚胎和 纸巾。这些流程中的每一个都需要建立和维护 具有重要细胞功能的亚稳定结构域。它们的作用机制 这些域的集合仍然不完全被理解，部分原因是不同的fi邪教 用目前的工具从功能上解剖动态蛋白质组件所固有的。 最近发展起来的光遗传工具允许光介导的蛋白质活性控制 具有高空间和时间分辨率的活细胞。这种新方法提供了一种 强大的手段来剖析这种潜在的分子机制 流程。我们将把改变特定fic分子活性的能力与高水平的 空间和时间分辨率与传统细胞生物学、成像、遗传学和 生化方法，以便更好地了解细胞如何分裂、极化和 塑造纸巾的形状。

Many essential cellular functions require large scale organization of the cell cortex. These include cytokinesis, asymmetric cell division, cell migration, and formation of an epithelium. Extensive organization of the cytoskeleton and cell-cell junctions are required during development to drive changes in the shape of cells, embryos, and tissues. Each of these processes requires establishment and maintenance of metastable domains that serve important cellular functions. The mechanisms by which these domains assemble remain incompletely understood, in part due to the difficulty inherent in functionally dissecting dynamic protein assemblies with current tools. Recently developed optogenetic tools permit light-mediated control of protein activity in live cells at high spatial and temporal resolution. This novel approach provides a powerful means to dissect the underlying molecular mechanism underlying such processes. We will combine the ability to alter the activity of specific molecules with high spatial and temporal resolution with conventional cell biology, imaging, genetic, and biochemical approaches, in order to better understand how cells divide, polarize and shape tissues.