POST TRANSLATIONAL MODIFICATION OF RHOA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 小的GTdR RhoA是胞质分裂的关键开关蛋白。在最近的一组实验中，我们发现，异位表达（和内源性，在较小程度上）RhoA是共价修饰和迁移约4kd的移动到一个较慢的流动性形式的SDS凝胶。RhoA是额外修饰的目标，其中许多修饰提供了重要的见解。我们建议通过质谱来表征这种修饰。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The small GTPase RhoA is a critical switch protein for cytokinesis. In a recent set of experiments, we found that ectopically expressed (and endogenous, to a lesser extent) RhoA is covalently modified and migrates with about a 4kd shift to a slower mobility form on a SDS gel. RhoA is a target for additional modifications and many of these have provided significant insight. We propose to characterize this modification by mass spectrometry.