Tsetse-microbiota negotiations mediated by microRNAs
Tsetse-microbiota negotiations mediated by microRNAs
批准号:
10452605
负责人:
Rita V M Rio
金额:
$19.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-07-16 至 2024-06-30
关键词:
AfricanAfrican TrypanosomiasisAgeAnimalsAnteriorBiocontrolsBiologicalBiologyBloodCoupledDataDevelopmentDietDigestionDipteraDistantDomestic AnimalsExperimental DesignsFemaleGastrointestinal tract structureGene TargetingGenesGenomicsGlossinidaeHomeostasisHumanImmunologicsIndigenousInsectaInvertebratesKnowledgeMaintenanceMediatingMediationMessenger RNAMetabolicMetabolismMicroRNAsMidgutMolecularNutrientOrganOutputPathogenicityPathway interactionsPeriodicityPest ControlPhysiologyPopulationPregnancyPublic HealthRNA libraryReproductionResearch DesignRoleSignal TransductionSmall RNASymbiosisTaxonomyTestingTrypanosomaTrypanosoma brucei bruceiTsetse FliesWigglesworthiaWild AnimalsWorkagedbacteriomebasecomparativecostdeep sequencingdensitydietarydifferential expressionfitnessflygut microbiotahost microbiotain vivolife historymalemembermicrobialmicrobiotanext generation sequencingnovelpregnantreproductivesexstomach cardiasymbionttraittranscriptome sequencingtransmission processvectorvector control
中文摘要
项目总结
英文摘要
Project summary
Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of African trypanosomes (Trypanosoma
brucei spp.) which are the causative agents of fatal Human African Trypanosomiasis (HAT), and Animal
African Trypanosomiasis (AAT) in wild and domesticated animals. The taxonomically simple tsetse
enteric microbiota is dominated by an obligate bacterial mutualist, Wigglesworthia. A monoculture of
intracellular Wigglesworthia is found within a specialized organ, known as the bacteriome, located at the
anterior midgut. Several critical roles towards tsetse biology, spanning from essential nutrient
provisioning to immunological priming, have been characterized for Wigglesworthia. Importantly, the
elimination (or functional disruption) of the tsetse- Wigglesworthia association results in lags to tsetse
development and sizeable reductions in reproductive output. Our long-term objective is to expand our
understanding of the molecular mechanisms that coordinate tsetse-Wigglesworthia activity. Here, the
role of tsetse-generated microRNAs (miRNAs) towards facilitating the metabolic and developmental
integration of Wigglesworthia-tsetse symbiosis will be examined. Our research design involves the
identification of miRNAs that are differentially expressed within bacteriomes (relative to non-symbiont
harboring organs) during tsetse sex-specific development and pregnancy using deep sequencing-based
approaches. Additionally, the gene targets of these miRNAs will be initially identified using
computational genomics. The effect(s) of disrupting miRNA-275, significantly upregulated within the
bacteriomes of pregnant tsetse, towards tsetse fly life history traits, bacteriome integrity and
Wigglesworthia density will be characterized. This project aims to unravel fundamental molecular
mechanisms that coordinate host-microbiota biology. The disruption of pivotal Wigglesworthia-mediated
metabolic processes within tsetse offers novel targets for biocontrol.
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会议论文
Tsetse-microbiota negotiations mediated by microRNAs
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批准号:10282112
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项目类别:
-
资助金额:$22.8万
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财政年份:2021
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负责人:Rita V M Rio
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依托单位:
Functional differentiation of tsetse species microbiota
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批准号:8940894
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项目类别:
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资助金额:$23.94万
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财政年份:2015
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负责人:Rita V M Rio
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依托单位:
Functional differentiation of tsetse species microbiota
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批准号:9265776
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项目类别:
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资助金额:$26.61万
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财政年份:2015
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负责人:Rita V M Rio
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依托单位:
Metabolic interdependency of tsetse fly symbiosis
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批准号:7984924
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项目类别:
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资助金额:$7.33万
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财政年份:2010
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负责人:Rita V M Rio
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依托单位:
Metabolic interdependency of tsetse fly symbiosis
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批准号:8072084
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项目类别:
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资助金额:$7.25万
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财政年份:2010
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负责人:Rita V M Rio
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依托单位:
海外基金