Tsetse-microbiota negotiations mediated by microRNAs

项目总结 采采蝇是非洲锥虫(锥虫)的循环媒介 (Brucei spp.)哪些是致命的人类非洲锥虫病(HAT)的病原体，以及动物 野生和家养动物中的非洲锥虫病(AAT)。分类上简单的采采集 肠道微生物区系主要由专性细菌互惠共生体Wigglesworth主宰。单一的文化 细胞内威格尔斯沃斯杆菌是在一种特殊的器官内发现的，这种器官被称为细菌体，位于 中肠前部。从必需营养素到采采树生物学的几个关键角色 对于威格尔斯沃思病来说，提供免疫启动是其特征。重要的是， TSETSE-Wigglesworth关联的消除(或功能中断)导致TSETSE滞后 发展和生殖产出的大幅减少。我们的长期目标是扩大我们的 了解协调采采草-威格尔斯沃斯菌活动的分子机制。在这里， 采采子产生的microRNAs(MiRNAs)在促进新陈代谢和发育中的作用 将研究威格尔斯沃西亚-采采子共生的整合。我们的研究设计涉及 细菌体(相对于非共生体)差异表达miRNAs的鉴定 基于深度测序的采采性特定发育和妊娠期间的 接近了。此外，这些miRNAs的基因靶标将通过以下方法初步确定 计算基因组学。干扰miRNA-275的作用(S)在体内显著上调 怀孕采采蝇的细菌体、采采蝇生活史特征、细菌体完整性和 威格尔斯沃斯密度将被描述为。这个项目的目的是解开基本分子 协调宿主-微生物区系生物学的机制。Wigglesworth介导的对关键基因的破坏 采采蝇体内的代谢过程为生物防治提供了新的靶点。

Project summary Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of African trypanosomes (Trypanosoma brucei spp.) which are the causative agents of fatal Human African Trypanosomiasis (HAT), and Animal African Trypanosomiasis (AAT) in wild and domesticated animals. The taxonomically simple tsetse enteric microbiota is dominated by an obligate bacterial mutualist, Wigglesworthia. A monoculture of intracellular Wigglesworthia is found within a specialized organ, known as the bacteriome, located at the anterior midgut. Several critical roles towards tsetse biology, spanning from essential nutrient provisioning to immunological priming, have been characterized for Wigglesworthia. Importantly, the elimination (or functional disruption) of the tsetse- Wigglesworthia association results in lags to tsetse development and sizeable reductions in reproductive output. Our long-term objective is to expand our understanding of the molecular mechanisms that coordinate tsetse-Wigglesworthia activity. Here, the role of tsetse-generated microRNAs (miRNAs) towards facilitating the metabolic and developmental integration of Wigglesworthia-tsetse symbiosis will be examined. Our research design involves the identification of miRNAs that are differentially expressed within bacteriomes (relative to non-symbiont harboring organs) during tsetse sex-specific development and pregnancy using deep sequencing-based approaches. Additionally, the gene targets of these miRNAs will be initially identified using computational genomics. The effect(s) of disrupting miRNA-275, significantly upregulated within the bacteriomes of pregnant tsetse, towards tsetse fly life history traits, bacteriome integrity and Wigglesworthia density will be characterized. This project aims to unravel fundamental molecular mechanisms that coordinate host-microbiota biology. The disruption of pivotal Wigglesworthia-mediated metabolic processes within tsetse offers novel targets for biocontrol.