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Establishment and Modulation of DNA Methylation Patterns in Arabidopsis

拟南芥 DNA 甲基化模式的建立和调控

基本信息

批准号：
10452655
负责人：
Julie Ann Law
金额：
$ 41.04万
依托单位：
SALK INSTITUTE FOR BIOLOGICAL STUDIES
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-15 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10452655
关键词：
Aberrant DNA Methylation Address Affect Animals Arabidopsis Biochemical Biological Models Biological Process Boundary Elements Cell physiology Cells Chemicals Chromatin Chromatin Remodeling Factor Complex Cytosine DNA DNA Methylation DNA-Directed RNA Polymerase Data Defect Deposition Development Developmental Process Disease Elements Ensure Environment Epigenetic Process Equilibrium Family Gene Expression Gene Expression Profile Gene Expression Regulation Gene Silencing Generations Genetic Genetic Materials Genetic Transcription Genome Growth and Development function Health Histones Homeostasis Human Immunoprecipitation Individual Lead Link Malignant Neoplasms Mammals Maps Mass Spectrum Analysis Methylation Modeling Modification Mouse-ear Cress Organism Pathway interactions Pattern Plant Model Plants Play Polymerase Positioning Attribute Process Production Progressive Disease Property Protein Family Proteins Reading Regulation Role Site Small Interfering RNA Source Specificity Speed System Techniques Testing Tissues Work base biochemical tools cell type chromatin modification complex IV epigenetic regulation epigenome epigenomics experimental study genome wide methylation genome-wide genome-wide analysis genomic tools imprint insight methylation pattern mutant novel predictive modeling preference prevent recruit response

项目摘要

Abstract Within any given organism, each cell has essentially identical genetic material, yet not all cells behave similarly. One source of this remarkable diversity is the presence of chemical tags, like DNA methylation, that decorate the genome and play roles in diverse biological processes including gene regulation, transposon silencing, and imprinting. While it is known that the patterns of DNA methylation can differ between tissues or cell types, how such patterns are generated and how they influence gene expression patterns remain poorly understood. As aberrant DNA methylation patterns are associated with developmental defects in plants and with numerous diseases in humans, understanding these aspects of epigenetic regulation are of critical importance. Using the plant model, Arabidopsis thaliana, the lab recently discovered a family of four related chromatin remodeling factors that control DNA methylation patterns in a locus- and tissue-specific manner. Based on new insights gained during the characterization of these chromatin regulators, this proposal seeks to understand the mechanisms that facilitate the locus-specific targeting of DNA methylation, to determine the checks and balances that enable genome-scale homeostasis within methylation pathways, and to investigate how genetic and epigenetic inputs are integrated to regulate DNA methylation patterns. Addressing these aspects of epigenetic regulation will not only be important for understanding the roles of DNA methylation during normal growth and development, but they will also provide insights into the causes and consequences of dysregulation within DNA methylation pathways. Arabidopsis thaliana is an ideal system to study epigenetic processes, like DNA methylation, as it is genetically malleable, has a small genome that is highly amenable to genome-wide analyses, and is tolerant of dramatic changes in its epigenetic landscape. In addition, many of the key players and pathways involved in establishing, maintaining, and reading epigenetic modifications are conserved between plants and mammals. Given these similarities, findings regarding how specific methylation patterns are generated and modulated during development, will be applicable to analogous processes in mammals.

摘要