Developing cell-penetrating miniproteins as a new class of therapeutics

开发细胞穿透微型蛋白作为一类新型疗法

• 批准号: 10454275
• 负责人: Gabriel Jacob Rocklin
• 金额: $ 19.16万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10454275
• 关键词: Address; Affinity; Amino Acids; Area; Autoimmunity; Binding Proteins; Biological Assay; Biology; Cell Line; Cell membrane; Cells; Cellular Membrane; Charge; Clinical; Cytolysis; Cytosol; Data; Development; Disease; Dose; Drug Targeting; Endosomes; Engineering; Ensure; Escherichia coli; Future; Goals; Hand; Human; Hydrophobicity; Incubated; Individual; Learning; Length; Libraries; Link; Lysosomes; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Measures; Medicine; Methods; Modeling; Peptides; Pharmaceutical Preparations; Plasma Cells; Property; Protein Engineering; Proteins; Proteomics; Recombinants; Recovery; Risk; Sampling; Structure; Structure-Activity Relationship; Surface; Technology; Testing; Therapeutic; Update; Ursidae Family; Validation; base; course development; design; drug development; improved; insight; iterative design; large scale data; machine learning prediction; multiplex assay; nanomolar; nervous system disorder; new therapeutic target; novel drug class; novel strategies; novel therapeutic intervention; novel therapeutics; peptide drug; pre-clinical; predictive modeling; protein protein interaction; small molecule; success; supervised learning; therapeutic protein

Project Summary Current protein therapeutics have a major limitation: they generally cannot cross the cellular membrane or interact with cytosolic targets. The ability to design protein therapeutics that enter the cell cytosol would enable new therapeutic strategies across many disease areas, including cancer, autoimmunity, and neurological disease. Therapeutic “miniproteins” (30-60 residues in length) have the potential to address this challenge, and several miniproteins capable of efficiently reaching the cell cytosol have recently been identified. However, we lack a general understanding of the “design rules” for cell-penetrating miniproteins, limiting the development of this class of molecules. Furthermore, current approaches to measure cytosolic delivery require measuring each protein individually, which is slow and labor intensive. This makes it impossible to test large numbers of miniproteins to develop a robust, quantitative understanding of the determinants of cytosolic delivery. In this exploratory project, we will develop a new approach to measure delivery for each different protein in a large mixed pool, using targeted mass spectrometry to individually identify each miniprotein sequence. In our approach, a soluble mixed pool containing thousands of designed miniprotein sequences is incubated with cells, and miniproteins that enter those cells are captured by a cytosolic target. Miniproteins captured by the target are then purified out of the cellular contents and identified and quantified using targeted proteomics. The amount of each protein in the captured sample (relative to the starting sample) will provide a quantitative measure of delivery efficiency. This approach is unprecedented, and we will test and optimize this approach using different positive and negative control miniproteins, different library sizes, and different cell lines. With this method in hand, we will use approaches we previously pioneered to computationally design thousands of candidate cell-penetrating miniproteins with intentionally diverse sequence and structural properties. We will then quantify cytosolic delivery for these new proteins using our new high-throughput approach, creating unprecedented large-scale data on delivery efficiency. We will then use these data to build machine learning models that predict miniprotein delivery based on sequence and structural properties. Finally, we will repeatedly iterate, designing new miniprotein libraries based on our predictive models of delivery, testing these designs using our high-throughput experimental approach, and further updating our models. This iterative design-test- learn approach will build a robust, predictive understanding of the determinants of delivery. Ultimately, the ability to design cell-penetrating miniproteins will unlock a wide range of new therapeutic targets inside the cell.

项目摘要 目前的蛋白质治疗剂具有一个主要的局限性：它们通常不能穿过细胞膜， 与胞质靶相互作用。设计进入细胞胞质溶胶的蛋白质治疗剂的能力将使 新的治疗策略跨越许多疾病领域，包括癌症、自身免疫和神经系统疾病。 疾病治疗性“微蛋白”（长度为30-60个残基）具有解决这一挑战的潜力， 最近已经鉴定了几种能够有效到达细胞胞质溶胶的微蛋白。但我们 缺乏对细胞穿透微蛋白的“设计规则”的一般理解，限制了 这类分子。此外，目前测量胞质递送的方法需要测量每个细胞的浓度。 蛋白质，这是缓慢和劳动密集型。这使得测试大量的 miniproteins开发一个强大的，定量的胞质传递的决定因素的理解。 在这个探索性的项目中，我们将开发一种新的方法来测量每种不同蛋白质的递送， 大混合池，使用靶向质谱法单独鉴定每个微蛋白序列。在我们 方法，将含有数千个设计的微蛋白序列的可溶性混合池与细胞一起孵育， 进入这些细胞的微蛋白被胞质靶捕获。被目标捕获的微蛋白是 然后从细胞内容物中纯化出来，并使用靶向蛋白质组学进行鉴定和定量。的量 捕获样品中的每种蛋白质（相对于起始样品）将提供 交付效率。这种方法是前所未有的，我们将使用不同的 阳性和阴性对照微蛋白、不同的文库大小和不同的细胞系。 有了这种方法，我们将使用我们以前开创的方法来计算设计数千个 候选细胞穿透miniproteins有意不同的序列和结构特性。我们将 然后使用我们新的高通量方法量化这些新蛋白质的胞质递送， 前所未有的大规模交付效率数据。然后我们将使用这些数据来构建机器学习 基于序列和结构特性预测微蛋白递送的模型。最后，我们将反复 根据我们的预测模型设计新的微蛋白库，测试这些设计， 使用我们的高通量实验方法，并进一步更新我们的模型。这种反复的设计测试- 学习方法将对交付的决定因素建立一个强有力的、可预测的理解。最终， 设计细胞穿透微蛋白将在细胞内开启一系列新的治疗靶点。