Recombinant Immunolabels for Nanoprecise Brain Mapping Across Scales

用于跨尺度纳米精确脑图谱的重组免疫标记

基本信息

  • 批准号:
    10454277
  • 负责人:
  • 金额:
    $ 140.12万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-09-30 至 2024-06-30
  • 项目状态:
    已结题

项目摘要

Recombinant Immunolabels for Nanoprecise Brain Mapping Across Scales Understanding brain function and dysfunction requires an understanding of the circuitry of the brain from molecules to cells to circuits. While no single technique can achieve this, a strategic combination of techniques applied across scales can provide information that when integrated can lead to a more complete picture. These techniques can range from analyses of single molecules, including their localization in nanodomains, to subcellular compartments such as synapses and synaptic networks to entire brains. One common theme of these techniques is that they require affinity probes that specifically label subcellular structures, cell types, and circuits within the brain. We propose to enhance an existing resource of renewable affinity probes in the form of an extensive collection of highly-validated monoclonal antibodies. We will enhance our ongoing dissemination of low cost, high quality antibodies to neuroscience researchers by converting these to recombinant form. This will also ensure permanence of this valuable collection of renewable reagents for future researchers. We will use established methods to miniaturize these conventional antibodies into a nanoscale form. These miniaturized antibodies will allow for labeling of brain targets with single nanometer precision, which will provide more spatially precise labeling and overcome the limits to imaging resolution that conventional Abs represent. Moreover, the miniaturized Abs will have much better sample penetration, allowing for more efficient labeling of larger samples of the type used for defining intact circuits. We have formed a consortium of experts who developed and/or are experts in a powerful set of advanced techniques that together allow for brain mapping across scales. This consortium will validate nanoscale antibodies in their respective techniques, a key component of rigor and reproducibility of any antibody-based research. We will also generate and validate novel nanoscale antibodies against targets of great interest to brain investigators for which no suitable reagents exist. Together, these efforts will further enhance the dissemination of this valuable resource, and fundamentally accelerate the pace of brain circuit mapping across scales.
纳米精确脑图谱的重组免疫标记

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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James S Trimmer其他文献

James S Trimmer的其他文献

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{{ truncateString('James S Trimmer', 18)}}的其他基金

Investigating the contributions of voltage gated sodium channels to oxaliplatin induced neuropathy
研究电压门控钠通道对奥沙利铂诱导的神经病变的影响
  • 批准号:
    10621059
  • 财政年份:
    2022
  • 资助金额:
    $ 140.12万
  • 项目类别:
Defining the Proteomic Composition of ER:Plasma Membrane Junctions in Brain Neurons
定义 ER 的蛋白质组组成:脑神经元的质膜连接
  • 批准号:
    9752682
  • 财政年份:
    2018
  • 资助金额:
    $ 140.12万
  • 项目类别:
UC Davis/NIH NeuroMab Facility
加州大学戴维斯分校/NIH NeuroMab 设施
  • 批准号:
    9511924
  • 财政年份:
    2015
  • 资助金额:
    $ 140.12万
  • 项目类别:
UC Davis/NIH NeuroMab Facility
加州大学戴维斯分校/NIH NeuroMab 设施
  • 批准号:
    9277589
  • 财政年份:
    2015
  • 资助金额:
    $ 140.12万
  • 项目类别:
UC Davis/NIH NeuroMab Facility
加州大学戴维斯分校/NIH NeuroMab 设施
  • 批准号:
    8956326
  • 财政年份:
    2015
  • 资助金额:
    $ 140.12万
  • 项目类别:
UC Davis/NIH NeuroMab Facility-Administrative Supplement
加州大学戴维斯分校/NIH NeuroMab 设施-行政补充
  • 批准号:
    9138371
  • 财政年份:
    2015
  • 资助金额:
    $ 140.12万
  • 项目类别:
Genetically Encoded Reporters of Integrated Neural Activity for Functional Mapping of Neural Circuitry-Administrative Supplement
用于神经回路功能图谱的综合神经活动的基因编码报告-管理补充
  • 批准号:
    9269378
  • 财政年份:
    2014
  • 资助金额:
    $ 140.12万
  • 项目类别:
Phosphorylation as a Determinant of BK Channel Expression and Localization
磷酸化作为 BK 通道表达和定位的决定因素
  • 批准号:
    7843641
  • 财政年份:
    2009
  • 资助金额:
    $ 140.12万
  • 项目类别:
NINDS/UC Davis NeuroMab Hybridoma Facility
NINDS/加州大学戴维斯分校 NeuroMab 杂交瘤设施
  • 批准号:
    7501090
  • 财政年份:
    2005
  • 资助金额:
    $ 140.12万
  • 项目类别:
UC Davis/NIH NeuroMab Facility
加州大学戴维斯分校/NIH NeuroMab 设施
  • 批准号:
    8113160
  • 财政年份:
    2005
  • 资助金额:
    $ 140.12万
  • 项目类别:

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阿伯丁大学和脊椎动物抗体有限公司 KTP 23_24 R1
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    Continuing Grant
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