Conscious ambulatory bladder monitoring to understand neural control of lower urinary tract function

有意识的动态膀胱监测以了解下尿路功能的神经控制

• 批准号: 10459673
• 负责人: DENNIS BOURBEAU
• 金额: $ 27.49万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10459673
• 关键词: Conscious; ambulatory; bladder; monitoring; understand

ABSTRACT Mechanisms underlying neural control of the lower urinary tract (LUT) in health and disease, including mechanisms of pathology development, are not well understood. New neurophysiology experiments to address knowledge gaps are being conducted in awake, behaving animals, rather than under anesthesia, but monitoring bladder function during chronic experiments remains a challenge. Current technologies can be used to wirelessly monitor bladder pressure via a surgically placed suprapubic catheter, but these technologies are limited because they do not measure bladder volume, which is an important variable; the catheter may irritate the bladder and confound the data; the receiver coil is not portable, which requires the animal to remain in a confined space for data collection; and the technology does not scale up to future human applications. A wireless, catheter-free bladder pressure and volume sensor is needed for chronic experiments in awake, behaving animals or humans to elucidate the neurophysiology of LUT function in health and disease. We have developed a prototype for a small, wireless, catheter-free pressure transducer that is rechargeable and can transmit continuous pressure data. We are building on this success by adding volume sensing and modifying the packaging for intravesical bladder placement. This new device, the Urological Monitor of Conscious Activity (UroMOCA), will be able to continuously measure and wirelessly transmit pressure and volume data from within the bladder to a small, wearable receiver unit. We have tested a potential packaging form factor in an animal bladder to demonstrate that it is not voided, nor does it obstruct bladder emptying. We have also tested an electrical conductance method of estimating volume wherein a very small current is passed between two outer electrodes and voltage is measured between two inner electrodes within the urine contained in the bladder, which we use to determine conductance from Ohm’s Law. By knowing the conductance, the conductivity of the urine, and the spacing between our electrodes, we were able to estimate bladder volume and this method can be incorporated into the UroMOCA. The goal of this proposal is to develop and validate the UroMOCA. The central hypothesis of this research is that the UroMOCA will wirelessly provide accurate bladder pressure and volume measurements for future neurophysiology experiments as validated by standard urodynamics tools and testing in conscious ambulating animals. The objectives of the proposed work are to (1) develop the UroMOCA for intravesical pressure and volume measurements by adding volume measurement to our previous design and repackaging the device into a new form factor and (2) validating the biocompatibility and function of the device by testing in acute nonsurvival and chronic ambulatory animal experiments. Completion of the proposed research will provide an important tool, which could be used in conjunction with nerve, electrocardiogram, electromyography or other recordings, for use by investigators studying the neural control of the lower urinary tract during conscious ambulatory experiments. It will also inform the design of next generation neuromodulation therapies to improve or restore LUT function, such as by providing closed-loop LUT control.

摘要 健康和疾病中下尿路（LUT）的神经控制机制，包括 病理学发展的机制还不清楚。新的神经生理学实验来解决 知识差距是在清醒的、有行为的动物身上进行的，而不是在麻醉下进行的， 在慢性实验期间监测膀胱功能仍然是一个挑战。目前的技术可以用于 通过手术放置的耻骨上导管无线监测膀胱压力，但这些技术 有限，因为它们不测量膀胱容量，这是一个重要的变量;导管可能刺激 膀胱和混淆数据;接收器线圈不是便携式的，这需要动物保持在一个 用于数据收集的有限空间;并且该技术不能扩展到未来的人类应用。一 无线、无导管的膀胱压力和容量传感器需要用于清醒的慢性实验， 行为动物或人类，以阐明LUT在健康和疾病中的功能的神经生理学。 我们已经开发出一种小型无线无导管压力传感器的原型， 可充电，可连续传输压力数据。我们正在这一成功的基础上， 感测和修改用于膀胱内膀胱放置的包装。这个新设备，泌尿科的 意识活动监测器（UroMOCA）将能够连续测量和无线传输 压力和体积数据从膀胱内传输到一个小型的可穿戴接收器单元。我们测试了一个 动物膀胱中的潜在包装形状因子，以证明其不会排尿，也不会阻塞 膀胱排空。我们还测试了一种估算体积的电导方法， 在两个外部电极之间通过小电流，并且在两个内部电极之间测量电压 在膀胱中的尿液中，我们用它来根据欧姆定律确定电导率。通过 知道了电导率，尿液的电导率，以及电极之间的间距， 以估计膀胱体积，并且该方法可以并入UroMOCA中。 本提案的目标是开发和验证UroMOCA。这项研究的核心假设是 UroMOCA将无线提供准确的膀胱压力和容量测量， 通过标准尿动力学工具和有意识走动中的测试验证的神经生理学实验 动物拟议工作的目标是（1）开发用于膀胱内压力的UroMOCA， 通过在我们以前的设计中增加体积测量并重新包装设备， 转换为新的外形尺寸，以及（2）通过在急性 非存活和慢性非卧床动物实验。完成拟议的研究将提供 重要工具，可与神经、心电图、肌电图或其他 记录，供研究人员在清醒时研究下尿路的神经控制 门诊实验它还将为下一代神经调节疗法的设计提供信息， 或恢复LUT功能，例如通过提供闭环LUT控制。

项目成果

High-density neural recordings from feline sacral dorsal root ganglia with thin-film array

使用薄膜阵列对猫骶骨背根神经节进行高密度神经记录

• DOI: 10.1088/1741-2552/abe398
• 发表时间: 2021
• 期刊: Journal of Neural Engineering
• 影响因子: 4
• 作者: Sperry, Zachariah J;Na, Kyounghwan;Jun, James;Madden, Lauren R;Socha, Alec;Yoon, Eusik;Seymour, John P;Bruns, Tim M
• 通讯作者: Bruns, Tim M

A Conductance-Based Sensor to Estimate Bladder Volume in Felines.

一种基于电导的传感器来估计猫科动物的膀胱体积。

• DOI: 10.1109/embc.2018.8512582
• 发表时间: 2018
• 期刊: Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
• 作者: McAdams,IanS;Majerus,SteveJA;Hanzlicek,Brett;Zorman,Christian;Bourbeau,Dennis;Damaser,MargotS
• 通讯作者: Damaser,MargotS

Sensors Selection for Continuous Monitoring of Bowel State and Activity.

用于连续监测肠道状态和活动的传感器选择。

• DOI: 10.1109/embc.2018.8512948
• 发表时间: 2018
• 期刊: Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
• 作者: Smiley,Aref;Majerus,SteveJA;McAdams,IanS;Hanzlicek,Brett;Bourbeau,Dennis;Damaser,MargotS
• 通讯作者: Damaser,MargotS

Low-cost, Implantable Wireless Sensor Platform for Neuromodulation Research.

用于神经调节研究的低成本、植入式无线传感器平台。

• DOI: 10.1109/biocas.2018.8584729
• 发表时间: 2018
• 期刊: IEEE Biomedical Circuits and Systems Conference : healthcare technology : [proceedings]. IEEE Biomedical Circuits and Systems Conference
• 作者: McAdams,Ian;Kenyon,Hannah;Bourbeau,Dennis;Damaser,MargotS;Zorman,Christian;Majerus,SteveJA
• 通讯作者: Majerus,SteveJA

Wireless and Catheter-Free Bladder Pressure and Volume Sensor.

无线、无导管膀胱压力和容量传感器。

• DOI: 10.1109/jsen.2023.3267749
• 发表时间: 2024
• 期刊: IEEE sensors journal
• 影响因子: 4.3
• 作者: Majerus,SteveJA;Hanzlicek,Brett;Hacohen,Yaneev;Cabal,Dario;Bourbeau,Dennis;Damaser,MargotS
• 通讯作者: Damaser,MargotS