In situ and digital spatial profiling of the active HIV reservoir in autopsy-derived tissues

尸检组织中活性 HIV 储存库的原位和数字空间分析

• 批准号: 10459933
• 负责人: Timothy Jensen Henrich
• 金额: $ 40.52万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10459933
• 关键词: Acute myocardial infarction; Anatomy; Autopsy; B-Lymphocytes; Biological Assay; Biological Markers; Biopsy; Brain; CD4 Positive T Lymphocytes; Cardiac; Cell Count; Cell Lineage; Cells; Cessation of life; Cryopreserved Tissue; DNA; Data; Drug or chemical Tissue Distribution; Environment; Formalin; Freezing; Frequencies; Funding; Gene Expression; Gene Expression Profiling; Genetic Transcription; HIV; Heart; Hemorrhage; Histologic; Human; Immune; Immunohistochemistry; Immunologics; In Situ; In Situ Hybridization; Individual; Investigation; Length; Liver; Lung; Lymphoid Cell; Lymphoid Tissue; Maintenance; Measures; Medical Examiners; Metabolic Clearance Rate; Morbidity - disease rate; Myelogenous; Myeloid Cells; National Heart, Lung, and Blood Institute; Neuroglia; Neurologic; Nucleic Acids; Organ; Overdose; Paraffin Embedding; Participant; Persons; Pharmaceutical Preparations; Phenotype; Proteins; Proteomics; Proviruses; Pulmonary Embolism; RNA; Recrudescences; Residual state; Resolution; Resources; Role; Sampling; San Francisco; Source; Spleen; Survival Rate; Time; Tissue Sample; Tissue-Specific Gene Expression; Tissues; Transcript; Viral; Virus; Withdrawal; antiretroviral therapy; base; cohort; comorbidity; digital; experience; immune activation; in vivo; innate immune pathways; innovation; interest; lymph nodes; macrophage; mortality; nano-string; novel; prospective; protein expression; sudden cardiac death; synergism; targeted treatment; transcriptomics; viral RNA; viral rebound

Project Summary/Abstract (Project 2) The study of HIV rebound potential necessitates rigorous viral and immunological characterization directly within tissues in people with HIV (PWH) on ART with minimal comorbidities. As a result, this project leverages the longitudinal San Francisco POstmortemSystematic InvesTigation of Sudden Cardiac Death (POST SCD) Study, a postmortem study to bank samples and autopsy data on PWH and uninfected controls who were victims of sudden cardiac death (SCD). To date we have collected extensive tissue samples, including brain, multiple lymph node chains, liver, spleen, heart, pulmonary vasculature and other tissues of interest from47 HIV-infected and >500 uninfected individuals who experienced SCD. Importantly, ~80% of HIV+ SCD cases were on ART and died suddenly of non-HIV (i.e. cardiac) causes. As a result, the HIV POST SCD cohort is a one-of-kind resource for the study of tissue HIV persistence. This highly innovative project involves in situ hybridization and cutting-edge tissue-based transcriptomic/proteomic nanoString Digital Spatial Profiling (DSP) to clearly define how the reservoirs of intact and HIV-expressing proviruses and biomarker expression differ between tissues and determine how tissue-specific differences in the transcriptionally active HIV reservoir relate to in situ host cell gene and protein expression. Our central hypothesis is that infected CD4 T cells and various myeloid lineage cells within immune privileged histologic environments express full-length, intact HIV RNA transcripts and be capable of rapid viral recrudescence following ART withdrawal. We expect to observe lower expression of host antiviral factors and higher expression of pro-survival factors in cell clusters expressing HIV transcripts. Furthermore, we posit that transcriptional activity and immune states of reservoir cells that are identified as predictors of viral rebound in Project 3 will be visualized within specific lymphoid tissue regions of interest. Our aims are to: 1) measure the total burden of intact and defective proviruses and HIV transcripts across the full spectrum of different organs and tissues in vivo; 2) compare across tissues the cellular burden and phenotypes of cells that spontaneously transcribe HIV transcripts in SCD victims on and off ART at the time of death; and 3) determine the in situ impact of HIV burden and residual transcriptional activity on host cell factors (particularly antiviral restriction and innate immune pathways) in tissue-resident lymphoid and myeloid cells in SCD victims on ART compared to uninfected controls. As a result, this project will have the capacity to identify targets for therapeutic approaches to achieve HIV cure in synergy with Projects 1 & 3.

项目摘要/摘要（项目二）