Nuclear Receptor Networks in Mucosal Immune Regulation
粘膜免疫调节中的核受体网络
基本信息
- 批准号:10459564
- 负责人:
- 金额:$ 51.19万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-30 至 2022-09-01
- 项目状态:已结题
- 来源:
- 关键词:AmplifiersAnti-Inflammatory AgentsAntigensBacteriaBile AcidsBile fluidBindingBiochemicalButyratesCD4 Positive T LymphocytesCell physiologyCellsColonDataDevelopmentDuodenumEffector CellEnteralEnterocytesEnterohepatic CirculationEnzymesEstrogensFOXP3 geneFoundationsGallbladderGene ExpressionGene Expression RegulationGenetic ScreeningGenetic TranscriptionGenomeGenomicsGlucocorticoid ReceptorHealthHepatocyteHistone DeacetylaseHomeostasisHumanIL10 geneImmuneImmune ToleranceImmunologicsImmunologyInflammationInflammatoryInflammatory Bowel DiseasesInterleukin-10IntestinesLarge IntestineLigand Binding DomainLigandsLinkLiverLocationLymphocyteLymphocyte SubsetMapsMetabolicMetabolismMicrobeMolecularMucosal ImmunityMucositisMucous MembraneMusNuclearNuclear Hormone ReceptorsNuclear ReceptorsOrganOrphanPeripheralProductionRNA InterferenceRNA interference screenReceptor ActivationRegulationRegulatory T-LymphocyteReportingResidual stateRoleSecondary toShapesSmall IntestinesT cell responseT-LymphocyteTestingThyroid HormonesTimeToxic effectUlcerative ColitisValidationVitamin D3 ReceptorVolatile Fatty AcidsWild Type MouseXenobioticsantagonistconstitutive androstane receptorcytokinedesignfunctional genomicshost-microbe interactionsileumimmunoregulationin vivolipophilicitymembermicrobialmicrobiotanovelnovel strategiesprogramsreceptorresponsescreeningsensorsmall bowel Crohn&aposs diseasesteroid hormonetargeted treatmenttranscription factor
项目摘要
Project Summary
The gut is a major immunological organ where host-microbe interactions shape both local and systemic immune
tolerance. However, current views of intestinal immune regulation ignore fundamental differences in the function
and metabolite composition of the two distinct organs that comprise the gut—the small and large intestine (or SI
and LI). This impedes a more detailed understanding of immune regulatory mechanisms along the intestinal
tract, and limits efforts to develop safer, more targeted treatments for the two major forms of human inflammatory
bowel diseases (IBDs), ulcerative colitis and small bowel Crohn’s disease. We hypothesize that mucosal CD4 T
cells use different sets of ligand-regulated nuclear receptors (NRs) in the SI and LI to control key regulatory
functions, including IL-10 expression, to local concentration gradients of bile- and microbe-derived metabolites.
On one hand, we have discovered that Foxp3– effector (Teff) subsets in the SI—including Th1 and Th17 cells—
utilize the nuclear xenobiotic receptor, constitutive androstane receptor (CAR/Nr1i3), to direct a ‘hepatocyte-like’
transcriptional response to contend with high local bile acid (BA) concentrations, which are far greater in SI than
in LI (millimolar vs micromolar) due to ‘enterohepatic’ circulation—where primary BAs synthesized in the liver,
stored in the gallbladder, and secreted into the duodenum are actively reabsorbed by specialized enterocytes in
the ileum for portal recirculation to the liver. Because BAs are lipophilic, they can be toxic and pro-inflammatory,
and several nuclear receptors—including CAR—have evolved to suppress BA toxicity. These studies suggest
that enterohepatic circulation establishes a unique SI microenvironment that is distinct from that in the LI and
requires unique transcriptional machinery to protect T cells in the SI. Conversely, the LI harbors 103-107 times
more bacteria than SI, and ~1000-fold lower BA concentrations. Accordingly, microbes and their metabolites—
short chain fatty acids (SCFAs; e.g., butyrate), secondary BAs (produced via microbial metabolism of residual
primary BAs)—are central to immune regulation in the LI. SCFAs inhibit histone deacetylase enzymes (HDACs)
and stabilize Foxp3 gene expression in peripherally-induced T regulatory cells (iTregs), whereas secondary BAs
appear to promote regulatory functions of RORgt+ Tregs in the LI through another NR, vitamin D receptor (VDR).
Thus, while antigens from the enteric flora prime both pro- and anti-inflammatory T cell responses throughout
the gut, marked concentration gradients of bile- and bacteria-derived metabolites in the SI vs. LI are sensed by
different NRs to execute compartmentalized T cell regulatory functions. In testing this hypothesis, we will apply
cutting-edge genomics and computational approaches to comprehensively map the contributions of each of the
49 mouse NRs to specialized regulatory functions in the SI and LI in vivo, using IL-10 expression as the primary
screening target. We will also interrogate the regulation and molecular functions of two key NRs, CAR/Nr1i3 and
VDR/Nr1i1, in SI type 1 regulatory (Tr1) and LI iTreg cells, respectively. These studies will advance
understanding of lymphocyte specialization in the gut, and inform new approaches to treat IBDs.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Matthew Eugene Pipkin其他文献
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{{ truncateString('Matthew Eugene Pipkin', 18)}}的其他基金
Nuclear Receptor Networks in Mucosal Immune Regulation
粘膜免疫调节中的核受体网络
- 批准号:
10822885 - 财政年份:2023
- 资助金额:
$ 51.19万 - 项目类别:
Nuclear Receptor Networks in Mucosal Immune Regulation
粘膜免疫调节中的核受体网络
- 批准号:
10591752 - 财政年份:2021
- 资助金额:
$ 51.19万 - 项目类别:
Nuclear Receptor Networks in Mucosal Immune Regulation
粘膜免疫调节中的核受体网络
- 批准号:
10283045 - 财政年份:2021
- 资助金额:
$ 51.19万 - 项目类别:
Transcription factor regulation of CD4 and CD8 T cell effector and memory differentiation and function
CD4 和 CD8 T 细胞效应及记忆分化和功能的转录因子调节
- 批准号:
10488579 - 财政年份:2020
- 资助金额:
$ 51.19万 - 项目类别:
shRNAmir and CRISPR sgRNA Library Construction Core
shRNAmir 和 CRISPR sgRNA 文库构建核心
- 批准号:
10591867 - 财政年份:2020
- 资助金额:
$ 51.19万 - 项目类别:
shRNAmir and CRISPR sgRNA Library Construction Core
shRNAmir 和 CRISPR sgRNA 文库构建核心
- 批准号:
10224890 - 财政年份:2020
- 资助金额:
$ 51.19万 - 项目类别:
shRNAmir and CRISPR sgRNA Library Construction Core
shRNAmir 和 CRISPR sgRNA 文库构建核心
- 批准号:
10024585 - 财政年份:2020
- 资助金额:
$ 51.19万 - 项目类别:
Transcription factor regulation of CD4 and CD8 T cell effector and memory differentiation and function
CD4 和 CD8 T 细胞效应及记忆分化和功能的转录因子调节
- 批准号:
10683256 - 财政年份:2020
- 资助金额:
$ 51.19万 - 项目类别:
Gene expression, Epigenetics and Bioinformatics Core
基因表达、表观遗传学和生物信息学核心
- 批准号:
10591868 - 财政年份:2020
- 资助金额:
$ 51.19万 - 项目类别:
shRNAmir and CRISPR sgRNA Library Construction Core
shRNAmir 和 CRISPR sgRNA 文库构建核心
- 批准号:
10488582 - 财政年份:2020
- 资助金额:
$ 51.19万 - 项目类别:
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