Genomics Core

项目概要/摘要 该计划项目申请的目的是深入了解基因组的分子基础 E box DNA 结合蛋白活性的广泛重塑，响应 TCR 信号，控制发育 γδ T 细胞的功能特化，这对于宿主防御至关重要。为了支持这项努力， Genomics Core 将提供对 scRNA-Seq、ChIP-Seq、ATAC-Seq 和 HiC 的集中访问 测序平台允许对转录因子以及表观遗传进行大规模基因组定位分析 用于揭示和描述 αβ 和 γδ T 谱系细胞染色质景观的标记。基因组学核心将 生成强大的单细胞转录组和小细胞数量表观遗传数据集，表观遗传景观为 以及用全局术语描述人类和小鼠 αβ 和 γδ T 谱系细胞的折叠模式的方法。 基因组学核心将协助参与实验室进行实验设计，并与 该计划的参与成员分析单个数据集以及组合数据集。基因组学 Core还将继续生成新的生物信息学管道来处理获得的全基因组数据集 来自大量群体以及从单个细胞获得的数据。最后，除了提供核心 服务，基因组学核心将成为研究生和博士后研究员的培训中心 深度 DNA 测序和相关生物信息学技术的利用 实验室。

PROJECT SUMMARY/ABSTRACT The objective of this program project application is to gain insight into the molecular basis by which genome- wide remodeling of E box DNA binding protein activity, in response to TCR signals, controls the development and functional specialization of γδ T cells, which are critically important to host defense. In supporting this effort, the Genomics Core would provide centralized access to scRNA-Seq, ChIP-Seq, ATAC-Seq and HiC sequencing platforms to allow large-scale genomic location analysis of transcription factors as well as epigenetic marks to reveal and describe the chromatin landscapes of αβ and γδ T-lineage cells. The Genomics Core would generate robust single-cell transcriptomic and small-cell-number epigenetic datasets, epigenetic landscapes as well as methods to describe the folding patterns of human and murine αβ and γδ T-lineage cells in global terms. The Genomics Core would assist the participating laboratories for experimental design and work closely with the participating members of the program to analyze individual, as well as, combined data sets. The Genomics Core will also continue to generate new bioinformatics pipelines to process genome-wide data sets obtained from bulk populations as well as data obtained from single cells. Finally, in addition to the provision of core services, the Genomics Core would be a hub for the training of graduate students and postdoctoral fellows in the utilization of deep DNA sequencing and associated bioinformatics techniques for the participating laboratories.