Genomics Core

项目摘要/摘要 这项计划项目申请的目标是深入了解基因组- 对TCR信号的响应，E盒DNA结合蛋白活性的广泛重塑控制着发育 以及γδT细胞的功能特化，这对宿主防御至关重要。在支持这一努力方面， 基因组学核心将提供对scRNA-Seq、ChIP-Seq、ATAC-Seq和HIC的集中访问 用于转录因子和表观遗传学大规模基因组定位分析的测序平台 揭示和描述αβ和γδT细胞染色质景观的标记。基因组学核心将 生成健壮的单细胞转录和小细胞数量表观遗传数据集，表观遗传景观如下 以及在全球范围内描述人类和小鼠αβ和γδT细胞系细胞折叠模式的方法。 基因组学核心将协助参与的实验室进行实验设计，并与 该计划的参与成员分析单独的以及组合的数据集。基因组学 CORE还将继续生成新的生物信息学管道，以处理获得的全基因组数据集 来自大量种群以及从单个细胞获得的数据。最后，除了提供核心 基因组学核心将成为培训研究生和博士后研究员的中心 利用深度DNA测序和相关生物信息学技术进行参与 实验室。

PROJECT SUMMARY/ABSTRACT The objective of this program project application is to gain insight into the molecular basis by which genome- wide remodeling of E box DNA binding protein activity, in response to TCR signals, controls the development and functional specialization of γδ T cells, which are critically important to host defense. In supporting this effort, the Genomics Core would provide centralized access to scRNA-Seq, ChIP-Seq, ATAC-Seq and HiC sequencing platforms to allow large-scale genomic location analysis of transcription factors as well as epigenetic marks to reveal and describe the chromatin landscapes of αβ and γδ T-lineage cells. The Genomics Core would generate robust single-cell transcriptomic and small-cell-number epigenetic datasets, epigenetic landscapes as well as methods to describe the folding patterns of human and murine αβ and γδ T-lineage cells in global terms. The Genomics Core would assist the participating laboratories for experimental design and work closely with the participating members of the program to analyze individual, as well as, combined data sets. The Genomics Core will also continue to generate new bioinformatics pipelines to process genome-wide data sets obtained from bulk populations as well as data obtained from single cells. Finally, in addition to the provision of core services, the Genomics Core would be a hub for the training of graduate students and postdoctoral fellows in the utilization of deep DNA sequencing and associated bioinformatics techniques for the participating laboratories.