权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Genomics Core

基因组学核心

基本信息

批准号：
8608281
负责人：
CORNELIS MURRE
金额：
$ 20.11万
依托单位：
RESEARCH INST OF FOX CHASE CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-15 至 2019-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8608281
关键词：
Antibodies Binding Binding Sites Bioinformatics Cell Lineage Cells Computer software Conceptions Core Facility DNA Sequence Data Data Analyses Data Set Development Enhancers Epigenetic Process Epithelial Generations Genes Genome Genomics Human Resources Immune System Diseases Individual Institution Laboratories Malignant Neoplasms Molecular Names Network-based Nuclear Pattern Play Population Postdoctoral Fellow Preparation Procedures Reagent Regulatory Element Research Research Infrastructure Research Personnel Research Proposals Role Sampling Services Signal Transduction Structure Sum T-Lymphocyte Technology Training Visit analytical tool base data acquisition enhancer binding protein genome wide association study genome-wide graduate student instrumentation new technology novel progenitor programs promoter research study sample fixation thymocyte tool transcription factor transcriptome sequencing

项目摘要

The objectives of the research proposals in this application are to determine at a global scale the mechanisms that underpin the αβ versus γδ lineage choice. Many of the experiments proposed in the application involve genome-wide analyses and interpretation of the data obtained from such analyses. The purpose of the Genomics Core is to provide the laboratories with the following services that will permit the proposed studies to be completed. The Genomics Core would perform HiC, ChlP-Seq and RNA-Seq analyses, followed by bioinformatics analysis. Thus the entire spectrum of genome-wide analyses that concerns this POl would be performed at the UCSD Genomics Core Facility. Why a centralized Genomics Core? There are three reasons: (1) Except for the Murre laboratory, none of the participating laboratories have expertise in ChlP-Seq and RNA-Seq. (2) The Zhuang, Wiest and Zuniga-Pflucker laboratories do not have access to the bioinformatic infrastructure and analytical tools that have been established at UCSD. (3) The genomics core would permit us to standardize the ChlP-Seq and RNA-Seq analysis. This means using the same antibodies, same lot number and the same approach thereby allowing a careful and consistent comparison of the different binding patterns that will be derived from the various studies. (4) The Genomics Core will provide training to visiting research fellows. Thus, we envision that graduate students and postdoctoral fellows from the other laboratories would visit UCSD in order to become familiar with global approaches and analyses. Accordingly, an integral part of our approach is to provide personnel from different institutions access to these global analysis tools and provide training.

本申请中的研究提案的目标是在全球范围内确定 α β与γ δ谱系选择的基础机制。许多实验中提出的，应用涉及全基因组分析和对从这种分析中获得的数据的解释。 Genomics Core的目的是为实验室提供以下服务，拟完成的研究。Genomics Core将执行HiC、ChIP-Seq和RNA-Seq 分析，然后进行生物信息学分析。因此，全基因组分析的整个范围，担心该POI将在UCSD基因组核心设施进行。为什么要集中基因组学核心？有三个原因：（1）除了Murre实验室，参与的实验室在ChIP-Seq和RNA-Seq方面具有专业知识。(2)壮族、维斯特和 Zuniga-Pflucker实验室无法使用生物信息基础设施和分析工具，在UCSD成立。(3)基因组学核心将允许我们标准化ChIP-Seq 和RNA-Seq分析。这意味着使用相同的抗体、相同的批号和相同的方法从而允许对将导出的不同结合模式进行仔细和一致的比较从各种研究中。(4)基因组学核心将为访问研究员提供培训。因此，我们设想来自其他实验室的研究生和博士后研究员将访问 UCSD，以熟悉全球方法和分析。因此，我们的方法是让来自不同机构的人员使用这些全球分析工具，提供培训。