Flow Cytometry Shared Resource

流式细胞术共享资源

• 批准号: 10461892
• 负责人: ANN M HABERMAN
• 金额: $ 8.81万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10461892
• 关键词: Antibodies; Biological Assay; Biological Response Modifier Therapy; Cancer Center Support Grant; Cell Separation; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Collection; Complex; Computer software; Consult; Consultations; Custom; Data; Data Analyses; Development; Emerging Technologies; Ensure; Epigenetic Process; Equipment; Event; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescence; Funding; Genes; Genetic; Genomics; Heterogeneity; Image; Immune; Individual; Laboratories; Licensing; Medical Research; Peptides; Performance; Phenotype; Population; Pre-Clinical Model; Preparation; Process; Proteins; Proteomics; Protocols documentation; Recovery; Research; Research Design; Research Personnel; Resource Sharing; Resources; Retrieval; Sampling; Services; Signal Pathway; Signal Transduction; Site; Sorting - Cell Movement; Stains; System; Techniques; Technology; Time; Training; Treatment Efficacy; Tumor Stem Cells; Yale Cancer Center; anticancer research; base; cancer cell; community center; cost; design; experience; extracellular; fluorophore; genomic RNA; indexing; innovation; instrument; medical schools; member; metabolomics; operation; programs; synthetic polymer Bioplex; tool; transcriptome sequencing; tumor immunology; tumor microenvironment; tumorigenesis

FLOW CYTOMETRY SHARED RESOURCE PROJECT SUMMARY/ABSTRACT Flow Cytometry Shared Resource was established in 2004 to provide ready and affordable access to state-of- the-art multi-parameter cell analysis and sorting capabilities. This facility is comprised of a large number and variety of flow cytometry instruments that quantify the fluorescence of individual cells, typically following staining with specific antibodies. Flow cytometry has become an important tool in cancer research for many different purposes. Some important applications include the immune profiling of tumor microenvironment and the phenotyping of cancer cells. Cell sorting by Flow Cytometry is critical for the purification of cells for genomics, proteomics, metabolomics, or the isolation of tumor stem cells. This institutional resource serves the entire Yale School of Medicine (YSM) campus, including Yale Cancer Center (YCC), and thus has a critical mass of users supporting these large and expensive instruments. During the most recent funding period, 197 laboratories have used Flow Cytometry. Ninety-four of these users (48%) were YCC members. This Shared Resource was most widely used by the Genomics, Genetics and Epigenetics (GGE) and the Cancer Immunology (CI) Research Programs. A wide variety of assays are performed, and the laboratory also offers consulting on experimental protocols with its experienced full-time director. Flow cytometry has multiple applications in cancer research. For example, as the pace of research into cancer immunology and biologic therapies increases, defining the functional and phenotypic heterogeneity of mixed cell populations will continue to be a critical technology in cancer research. Flow cytometry is not only essential for cancer immunology research, but also empowers numerous other types of studies such as oncogenesis, cell signaling events, preclinical models, the assessment of therapeutic efficacy, gene editing using CRISPR and of course, cell sorting for genomic and RNA sequencing. Clearly, Flow Cytometry is vital to a number of programs within YCC. The Specific Aims of Flow Cytometry are to: (1) Provide flow cytometry services that are of high quality, readily available, and at reasonable cost; (2) Educate the YCC community on the applications of flow cytometry in medical research; and (3) Collaborate with YCC investigators in the development of new applications.

流式细胞仪共享资源 项目总结/摘要 流式细胞术共享资源成立于2004年，提供现成的和负担得起的访问状态， 先进的多参数细胞分析和分选能力。该设施由大量的和 各种流式细胞仪，其定量单个细胞的荧光，通常如下 用特异性抗体染色。流式细胞术已成为许多癌症研究的重要工具， 不同的目的一些重要的应用包括肿瘤微环境的免疫谱分析， 癌细胞的表型分析通过流式细胞术进行细胞分选对于纯化细胞以用于细胞移植是至关重要的。 基因组学、蛋白质组学、代谢组学或肿瘤干细胞的分离。这一机构资源服务于 整个耶鲁医学院（YSM）校园，包括耶鲁癌症中心（YCC），因此有一个关键的 大量用户支持这些大型和昂贵的仪器。 在最近的资助期间，197个实验室使用了流式细胞术。其中94名用户 (48%）是YCC成员。这种共享资源被基因组学、遗传学和 表观遗传学（GGE）和癌症免疫学（CI）研究计划。各种各样的测定法是 执行，实验室还提供咨询的实验方案，其经验丰富的全职 导演 流式细胞术在癌症研究中有多种应用。例如，随着癌症研究的步伐 免疫学和生物学疗法的增加，定义了混合免疫系统的功能和表型异质性。 细胞群将继续是癌症研究中的关键技术。流式细胞术不仅必不可少 用于癌症免疫学研究，而且还支持许多其他类型的研究，如肿瘤发生， 细胞信号传导事件，临床前模型，疗效评估，使用CRISPR进行基因编辑 当然，还有细胞分选，用于基因组和RNA测序。显然，流式细胞术对于许多 YCC内的节目。 流式细胞术的具体目标是：（1）提供高质量、快速、准确的流式细胞术服务。 （2）向YCC社区宣传流式细胞术在以下方面的应用： 医学研究;（3）与YCC研究人员合作开发新的应用。