Core A: Cell Model and Evaluation Core
核心A:细胞模型和评估核心
基本信息
- 批准号:10468804
- 负责人:
- 金额:$ 24.17万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-04-01 至 2023-04-30
- 项目状态:已结题
- 来源:
- 关键词:AdoptedAnatomyAnimal ModelAreaAwardBiological AssayBiologyBronchiCell Culture SystemCell Culture TechniquesCell modelCellsCellular AssayClinical TrialsComplementComplexCost SavingsCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDevelopmentDiseaseDyesEpithelialEpithelial CellsEquipmentEvaluationEvolutionFosteringFrequenciesFunctional disorderFundingGoldHealth Insurance Portability and Accountability ActHumanHuman ResourcesHydration statusImageIn SituIn VitroIndividualInstitutional Review BoardsInternationalInterventionIon TransportIonsLaboratoriesLaboratory ResearchLung TransplantationMeasurementMeasuresMessenger RNAMethodologyMethodsModelingMorbidity - disease rateMucociliary ClearanceMucous body substanceNoseOperative Surgical ProceduresOptical Coherence TomographyPathogenesisPathway interactionsPermeabilityPhenotypeProcessPropertyPulmonary Cystic FibrosisReagentReproducibilityResearchResearch DesignResearch PersonnelResearch SupportResolutionResourcesRightsSafetySamplingScienceScientistSourceStandardizationSurfaceSystemTechniquesTechnologyTestingThickTissuesTracheaTrainingTranslatingTranslationsUnited States National Institutes of HealthValidationViscosityWestern BlottingWorkairway epitheliumairway inflammationairway surface liquidanatomic imagingcystic fibrosis patientsdigitaldrug discoveryexperiencehuman subjectimprovedin vivoinnovationinsightmaterial transfer agreementmortalitynovelnovel therapeuticsprotein expressionquality assuranceranpirnasereal-time imagesrepositoryresponsesample fixationstandard measure
项目摘要
PROJECT SUMMARY / ABSTRACT: P30 CORE A
Well-differentiated primary human airway epithelial cells and the assays that can be used with them are an
instrumental model for understanding epithelial biology and are highly predictive of in vivo results in clinical
trials. Primary cells can be used with methodologies that translate readily to assays of airway function in vivo,
including measures of CFTR activity or other ion transporters, airway surface liquid depth and mucus
hydration, and mucus viscosity and transport. Primary airway cells further provide an excellent model for
examining the biology of airway epithelial inflammation, which is key to the pathogenesis of cystic fibrosis (CF)
lung disease. The purpose of Core A is to support the research of numerous P30 investigators that involves
cell culture systems and to assist with established and innovative assays available to characterize cellular
responses.
Core A carries out three main functions as outlined in the Specific Aims. First, Core A procures, grows, and
distributes well-differentiated primary human airway epithelial cells from CF and non-CF donors. This includes
samples of cells from lung transplants, surgically excised nasal tissue, and nasal brushings obtained at UAB
and from a large array of collaborating centers. Examination of novel expansion and differentiation techniques
for primary cells is also a key function of the Core. Second, Core A conducts functional anatomic imaging of
airway epithelia by 1-micron resolution Optical Coherence Tomography (μOCT) in vitro (primary cells of human
or non-human origin) and ex vivo (intact full-thickness trachea or mainstem bronchi of human origin or
comparable tissues from CF animal models, thus interfacing with Core B). μOCT allows for investigators to
explore mucus flow and mucociliary interactions and is designated as a National Core due to its unique and
important capabilities. Third, Core A performs and assists with measures of CFTR activity and expression. In
addition to traditional assays of CFTR function (e.g, Ussing chambers), the Core supports innovative
conductance assays and advanced PCR technology for investigating CFTR and other protein expression.
Core A facilitates interdisciplinary collaborative research, provides resources that are beyond the expertise of
individual research laboratories, fosters the sharing of ideas and experimental strategies, assists with technical
troubleshooting, and maintains essential equipment that is and will be heavily utilized by P30 personnel and
beyond. Cost savings are achieved by minimizing duplicate efforts of individual CF investigators, by the
centralized purchase and usage of equipment, reagents, and supplies, as well as by maintaining a central
repository for human epithelial tissue. On the whole, Core A provides significant expertise and resources to
aid P30 investigators, fostering advancement of epithelial cell culture and innovative assays to understand
CFTR pathogenesis and support rational drug discovery.
项目总结/摘要:P30核心A
分化良好的原代人气道上皮细胞和可以与它们一起使用的测定法是一种新的方法。
用于理解上皮生物学的工具模型,并高度预测临床中的体内结果
审判原代细胞可以与容易转化为体内气道功能测定的方法一起使用,
包括测量CFTR活性或其他离子转运蛋白、气道表面液体深度和粘液
水化和粘液粘度和运输。初级气道细胞进一步提供了一个极好的模型,
检查气道上皮炎症的生物学,这是囊性纤维化(CF)发病机制的关键
肺病。核心A的目的是支持众多P30研究者的研究,
细胞培养系统,并协助建立和创新的分析,可用于表征细胞
应答
核心A执行具体目标中概述的三个主要功能。首先,核心A采购,增长,
分布来自CF和非CF供体的分化良好的原代人气道上皮细胞。这包括
来自肺移植、手术切除的鼻组织和在UAB获得的鼻刷的细胞样本
and from a large大array阵列of collaborating合作centers中心.新的扩展和分化技术的检查
也是核心的关键功能。第二,核心A进行功能解剖成像,
1微米分辨率光学相干断层扫描(μOCT)的体外气道上皮细胞(人的原代细胞
或非人来源)和离体(人来源的完整全层气管或主干支气管,或
来自CF动物模型的可比较组织,因此与核心B接合)。μOCT允许研究者
探索粘液流动和粘液纤毛的相互作用,并被指定为国家核心,由于其独特的,
重要的能力。第三,核心A执行并协助CFTR活动和表达的测量。在
除了传统的CFTR功能测定(例如,Ussing室)外,Core还支持创新的
电导测定和先进的PCR技术用于研究CFTR和其他蛋白质表达。
核心A促进跨学科的合作研究,提供超出专业知识的资源,
个人研究实验室,促进思想和实验策略的共享,协助技术
排除故障,维护P30人员大量使用的必要设备,
超越。通过最大限度地减少个别CF调查员的重复工作,
集中采购和使用设备、试剂和供应品,以及维持一个
人类上皮组织的储存库。总体而言,核心A提供了重要的专业知识和资源,
帮助P30研究人员,促进上皮细胞培养和创新检测的进步,以了解
CFTR发病机制和支持合理的药物发现。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Bradford Alan Woodworth其他文献
10:48: Spontaneous CSF Leaks: A Paradigm for Definitive Repair
- DOI:
10.1016/j.otohns.2007.06.031 - 发表时间:
2007-08-01 - 期刊:
- 影响因子:
- 作者:
Bradford Alan Woodworth;Anthony Alexander Prince;Alexander G. Chiu;Noam A. Cohen;David W. Kennedy;James N. Palmer - 通讯作者:
James N. Palmer
Bradford Alan Woodworth的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Bradford Alan Woodworth', 18)}}的其他基金
A Ginsenoside TMEM16A Potentiator for Cystic Fibrosis
人参皂苷 TMEM16A 治疗囊性纤维化的增效剂
- 批准号:
10574384 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Ivacaftor for Acquired CFTR Dysfunction in Chronic Rhinosinusitis
Ivacaftor 治疗慢性鼻窦炎获得性 CFTR 功能障碍
- 批准号:
9324344 - 财政年份:2016
- 资助金额:
$ 24.17万 - 项目类别:
Chloride secretagogues for acquired CFTR dysfunction in chronic rhinosinusitis
氯化物促分泌剂治疗慢性鼻窦炎获得性 CFTR 功能障碍
- 批准号:
8233957 - 财政年份:2011
- 资助金额:
$ 24.17万 - 项目类别:
Chloride secretagogues for acquired CFTR dysfunction in chronic rhinosinusitis
氯化物促分泌剂治疗慢性鼻窦炎获得性 CFTR 功能障碍
- 批准号:
8620702 - 财政年份:2011
- 资助金额:
$ 24.17万 - 项目类别:
Chloride secretagogues for acquired CFTR dysfunction in chronic rhinosinusitis
氯化物促分泌剂治疗慢性鼻窦炎获得性 CFTR 功能障碍
- 批准号:
8028013 - 财政年份:2011
- 资助金额:
$ 24.17万 - 项目类别:
Chloride secretagogues for acquired CFTR dysfunction in chronic rhinosinusitis
氯化物促分泌剂治疗慢性鼻窦炎获得性 CFTR 功能障碍
- 批准号:
8433329 - 财政年份:2011
- 资助金额:
$ 24.17万 - 项目类别:
相似海外基金
Linking Epidermis and Mesophyll Signalling. Anatomy and Impact in Photosynthesis.
连接表皮和叶肉信号传导。
- 批准号:
EP/Z000882/1 - 财政年份:2024
- 资助金额:
$ 24.17万 - 项目类别:
Fellowship
Digging Deeper with AI: Canada-UK-US Partnership for Next-generation Plant Root Anatomy Segmentation
利用人工智能进行更深入的挖掘:加拿大、英国、美国合作开发下一代植物根部解剖分割
- 批准号:
BB/Y513908/1 - 财政年份:2024
- 资助金额:
$ 24.17万 - 项目类别:
Research Grant
Doctoral Dissertation Research: Social and ecological influences on brain anatomy
博士论文研究:社会和生态对大脑解剖学的影响
- 批准号:
2235348 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Standard Grant
Simultaneous development of direct-view and video laryngoscopes based on the anatomy and physiology of the newborn
根据新生儿解剖生理同步开发直视喉镜和视频喉镜
- 批准号:
23K11917 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Computational comparative anatomy: Translating between species in neuroscience
计算比较解剖学:神经科学中物种之间的翻译
- 批准号:
BB/X013227/1 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Research Grant
computational models and analysis of the retinal anatomy and potentially physiology
视网膜解剖学和潜在生理学的计算模型和分析
- 批准号:
2825967 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Studentship
Genetics of Extreme Phenotypes of OSA and Associated Upper Airway Anatomy
OSA 极端表型的遗传学及相关上呼吸道解剖学
- 批准号:
10555809 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Development of a novel visualization, labeling, communication and tracking engine for human anatomy.
开发一种新颖的人体解剖学可视化、标签、通信和跟踪引擎。
- 批准号:
10761060 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Understanding the functional anatomy of nociceptive spinal output neurons
了解伤害性脊髓输出神经元的功能解剖结构
- 批准号:
10751126 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
The Anatomy of Online Reviews: Evidence from the Steam Store
在线评论剖析:来自 Steam 商店的证据
- 批准号:
2872725 - 财政年份:2023
- 资助金额:
$ 24.17万 - 项目类别:
Studentship