Core C: Animal Models Core

项目摘要：核心C，动物模型核心 目前帕金森氏病(PD)的治疗方法可以缓解症状，但不能阻止 这种疾病。寻找新的治疗靶点以防止疾病的进展需要动物模型 这概括了帕金森氏症的特点。帕金森病的α-SYN纤维模型复制了 疾病，如黑质致密部包涵体、多巴胺神经元丢失和运动缺陷。 核心C：动物模型核心的目标是标准化该模型以生成一致的结果 横跨所有项目。动物模型核心将纯化并产生低内毒素重组α-SYN，产生 并进行质量控制分析，如沉淀法和硫代黄素T法，动态光 散射和透射电子显微镜。动物模型核心还将执行所有 立体定向注射以最小化立体定向技术中的可变性。灌流、组织切片和 还将进行免疫组织学技术和无偏见的体视学。受过高度训练 执行这些技术的核心人员将产生高质量的数据，并将潜力降至最低 可变性。

Project Summary: Core C, Animal Models Core Current treatments for Parkinson's disease (PD) alleviate the symptoms, but do not prevent the progression of the disease. Finding novel therapeutic targets to prevent the progression of the disease requires animal model that recapitulate the features of PD. The α-syn fibril model of Parkinson Disease replicates features of the disease such as inclusions in the substantia nigra pars compacta, dopamine neuron loss and motor defects. The goal of the Core C: Animal Models Core is to standardize this models to generate consistent results across the projects. Animal Models Core will purify and generate low endotoxin recombinant α-syn, generate fibrils and perform quality control assays such as sedimentation and Thioflavin T assays, dynamic light scattering and transmission electron microscopy. The Animal Models Core will also perform all of the stereotactic injections to minimize variability in stereotactic technique. Perfusions, tissue sections and immunohistological techniques and unbiased stereology will also be performed. Having highly trained personnel from the Core perform these techniques will produce high quality data and will minimize potential variability.