Core C: Animal Models Core

核心 C：动物模型核心

• 批准号: 9976622
• 负责人: Laura A. Volpicelli-Daley
• 金额: $ 24.63万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9976622
• 关键词: Amygdaloid structure; Animal Model; Animals; Autopsy; Biological Assay; Brain; Brain Death; Brain region; Crossbreeding; Data; Defect; Detergents; Disease; Disease Progression; Electron Microscopy; Endotoxins; Ensure; Escherichia coli; Excision; Filament; Genes; Goals; Human Resources; Immunofluorescence Immunologic; Immunohistochemistry; Individual; Injections; LRRK2 gene; Lewy Bodies; Lewy neurites; Measurement; Mediating; Modeling; Motor; Natural Immunity; Nature; Neurons; Operative Surgical Procedures; Parkinson Disease; Pathologic; Pathology; Pathway interactions; Patients; Perfusion; Phenotype; Plant Resins; Play; Proteins; Protocols documentation; Publishing; Quality Control; Rattus; Recombinants; Reproducibility; Research; Research Personnel; Rodent; Sampling; Sedimentation process; Serine; Services; Site; Standardization; Substantia nigra structure; Techniques; Tissues; Training; Transgenic Organisms; Transmission Electron Microscopy; Tyrosine 3-Monooxygenase; adaptive immunity; alpha synuclein; brain tissue; cost; dopaminergic neuron; experience; improved; interest; light scattering; light transmission; macrophage; neuron loss; new therapeutic target; novel therapeutics; pars compacta; prevent; reduce symptoms; response; skills; tool

Project Summary: Core C, Animal Models Core Current treatments for Parkinson's disease (PD) alleviate the symptoms, but do not prevent the progression of the disease. Finding novel therapeutic targets to prevent the progression of the disease requires animal model that recapitulate the features of PD. The α-syn fibril model of Parkinson Disease replicates features of the disease such as inclusions in the substantia nigra pars compacta, dopamine neuron loss and motor defects. The goal of the Core C: Animal Models Core is to standardize this models to generate consistent results across the projects. Animal Models Core will purify and generate low endotoxin recombinant α-syn, generate fibrils and perform quality control assays such as sedimentation and Thioflavin T assays, dynamic light scattering and transmission electron microscopy. The Animal Models Core will also perform all of the stereotactic injections to minimize variability in stereotactic technique. Perfusions, tissue sections and immunohistological techniques and unbiased stereology will also be performed. Having highly trained personnel from the Core perform these techniques will produce high quality data and will minimize potential variability.

项目总结：核心C，动物模型核心 目前帕金森病（PD）的治疗缓解了症状，但不能阻止帕金森病的进展。 这种疾病寻找新的治疗靶点以阻止疾病的进展需要动物模型 概括了帕金森病的特征。帕金森病的α-syn纤维模型复制了帕金森病的特征。 疾病，如黑质中的内含物，多巴胺神经元损失和运动缺陷。 核心C：动物模型核心的目标是标准化该模型，以生成一致的结果 跨项目。动物模型核心将纯化并产生低内毒素重组α-syn， 纤维和执行质量控制测定，如沉降和硫磺素T测定，动态光 散射和透射电子显微镜。动物模型核心还将执行所有 立体定向注射以最小化立体定向技术中的可变性。灌注、组织切片和 还将进行免疫组织学技术和无偏体视学。训练有素的 来自美国核心的人员执行这些技术将产生高质量的数据，并将最大限度地减少潜在的 可变性