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Flow Cytometry and Cell Sorting Shared Resource Facility

流式细胞术和细胞分选共享资源设施

基本信息

批准号：
10470107
负责人：
DONALD A COHEN
金额：
$ 11.43万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-08 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10470107
关键词：
Address Animals Antibodies Apoptosis Applications Grants Basic Science Bioinformatics Biological Assay Biological Markers Biometry Blood Cancer Center Cancer Center Support Grant Cancer Science Catchment Area Cell Cycle Cell Separation Cell physiology Cells Clinical Clinical Cancer Center Clinical Research Clinical Trials Cloning Collaborations Complex Computers and Advanced Instrumentation Consult Consultations Core Facility Cryopreservation Data Data Analyses Dendritic Cells Development Doctor of Philosophy Enzyme-Linked Immunosorbent Assay Experimental Designs Faculty Flow Cytometry Flow Cytometry Shared Resource Fresh Tissue Funding Generations Goals Grant Growth Factor Health Human Immune Immune system Immunologic Monitoring Immunologics Immunology procedure Individual Informatics Kentucky Laboratories Leadership Malignant Neoplasms Mentors Mentorship Mission Monitor National Cancer Institute Necrosis Pathology Peer Review Persons Phenotype Ploidies Population Production Publications Research Research Design Research Personnel Research Support Resource Sharing Sampling Services Signal Transduction Speed Surface System T-Cell Proliferation T-Lymphocyte Techniques Tissue Expansion Training Tumor Tissue United States National Institutes of Health Universities anticancer research antigen-specific T cells base cancer biomarkers cancer immunotherapy career cell bank cost effective cytokine cytotoxicity design enzyme linked immunospot assay improved innovation instrument instrumentation macrophage meetings member multidimensional data novel operation phenotypic biomarker pre-clinical pre-clinical research programs tumor immunology

项目摘要

PROJECT SUMMARY/ABSTRACT The Flow Cytometry and Cell Sorting Shared Resource Facility (FCCS SRF) facilitates high impact cancer science within the Markey Cancer Center (MCC) and is aided by current CCSG funding. The FCCS SRF has been in continuous operation at the University of Kentucky (UK) since 1983 and under the direction of Donald Cohen, PhD, since 1992. Although an institutional core facility at UK, during the current period of CCSG funding, 68% of users were MCC members, and 53% of users were peer-reviewed funded MCC members. To support research efforts of MCC members, the FCCS SRF provides state-of-art flow cytometric services and comprehensive immune monitoring services to support cancer research by all 4 Research Programs. A variety of flow cytometric services, including multi-parametric cell phenotyping (up to 12 biomarkers simultaneously), allows users to analyze surface and intracellular biomarkers, DNA content, cell cycle, apoptosis and necrosis, cytokine/growth factor synthesis, signal transduction and cell activation. Under guidance by MCC leadership, the FCCS SRF recently expanded services to provide MCC members comprehensive immune monitoring for human basic and clinical cancer research. Immune monitoring services include processing fresh blood and tumor tissue for isolation of immune and non-immune cells for functional assay and cryopreservation and banking of cells for later analysis. Banking services are provided in collaboration with the Biospecimen Procurement and Translational Pathology SRF and the Cancer Research Informatics SRF. Freshly isolated and banked cell samples can be analyzed for a number of cellular functions including synthesis of cytokines/growth factors/other soluble factors by ELISA, ELISPOT and Luminex multiplexing, in addition to flow cytometric approaches. Assays are also offered to quantify antigen-specific T cell proliferation, cytotoxicity, antibody synthesis and cytokine production in human systems. Cellular product services include generation of dendritic cells and macrophages from blood and/or tumor tissue and expansion of T cells from blood and/or tumor tissue. In collaboration with the Biostatistics and Bioinformatics SRF, FCCS offers high dimensional data analysis for multi-parameter flow cytometry and Luminex multi-analyte profiling for identification of complex cell populations and rare cell subsets. Over the previous CCSG funding period, FCCS services have contributed to 118 publications, 49 grants funded during the last four years, and has initiated consultation and collaboration on 4 clinical trials by MCC members. The comprehensive flow cytometry and immune monitoring services offered by FCCS, in combination with FCCS immune assay design services, are providing MCC researchers with the exceptional opportunities to interrogate novel cancer biomarkers and the immune system interactions in studies involving basic and clinical cancer research at the MCC.

项目摘要/摘要流式细胞术和细胞分选共享资源设施(FCCS SRF)促进高影响癌症这是马基癌症中心(MCC)内的一项科学研究，并得到CCSG目前的资助。FCCS SRF拥有自1983年以来一直在肯塔基大学(英国)运作，并在唐纳德的指导下科恩，博士，自1992年以来。尽管英国的一个机构核心设施，在CCSG的当前时期在资金方面，68%的用户是MCC成员，53%的用户是同行审查资助的MCC成员。至支持MCC成员的研究努力，FCCS SRF提供最先进的流式细胞仪服务和全面的免疫监测服务，支持所有4个研究项目的癌症研究。品种繁多流式细胞仪服务，包括多参数细胞表型(同时多达12个生物标记物)，允许用户分析表面和细胞内的生物标记物、DNA含量、细胞周期、细胞凋亡和坏死，细胞因子/生长因子合成、信号转导和细胞激活。在MCC领导的指导下， FCCS SRF最近扩大了服务范围，为MCC成员提供全面的免疫监测人类基础和临床癌症研究。免疫监测服务包括处理新鲜血液和用于免疫和非免疫细胞分离的肿瘤组织，用于功能分析和冷冻保存将细胞储存起来，以供以后分析。银行服务是与Biosecimen合作提供的采购和翻译病理学SRF和癌症研究信息学SRF。新隔离的可以分析储存的细胞样本的许多细胞功能，包括合成细胞因子/生长因子/其他可溶性因子通过ELISAT、ELISPOT和Luminex多重分析，以及Flow 细胞学方法。还提供了检测抗原特异性T细胞增殖、细胞毒性、人体系统中抗体合成和细胞因子的产生。蜂窝产品服务包括生成来自血液和/或肿瘤组织的树突状细胞和巨噬细胞以及来自血液和/或肿瘤组织的T细胞的扩增肿瘤组织。FCCS与生物统计和生物信息学SRF合作，提供高维数据多参数流式细胞术和Luminex多分析物图谱在复杂细胞鉴定中的应用种群和稀有细胞亚群。在前一个CCSG资助期内，FCC服务有助于 118份出版物，49份赠款，在过去四年中得到资助，并发起了协商和合作关于MCC成员的4项临床试验。全面的流式细胞术和免疫监测服务由FCCS提供，结合FCCS免疫分析设计服务，正在为MCC研究人员提供有机会询问新的癌症生物标记物和免疫系统的相互作用参与MCC的基础和临床癌症研究。