PROJECT 6. Flow Cytometry

项目 6. 流式细胞术

• 批准号: 8740615
• 负责人: DONALD A COHEN
• 金额: $ 7.71万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-08 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8740615
• 关键词: Address; Animals; Apoptosis; Basic Science; Biological Assay; Cancer Center; Cancer Center Support Grant; Cancerous; Cell Cycle; Cell Separation; Cells; Characteristics; Clinical Research; Clone Cells; Data Analyses; Development; Faculty; Flow Cytometry; Funding Agency; Future; Goals; Human; Institutes; Kentucky; Life; Malignant Neoplasms; Measurement; NCI Center for Cancer Research; Necrosis; Normal Cell; Output; Phenotype; Ploidies; Publications; Research; Research Personnel; Research Project Grants; Resource Sharing; Sampling; Services; Signal Transduction; Sorting - Cell Movement; Speed; Techniques; Tissues; Training; Translational Research; United States National Institutes of Health; Universities; Update; anticancer research; cytokine; cytotoxicity; instrument; instrumentation; member; operation; programs

The Markey Cancer Center (MCC) Flow Cytometry and Cell Sorting Shared Resource Facility (FCCS SRF) provides University of Kentucky (UK) cancer researchers with comprehensive, state-of-the-art analytical flow cytometry and cell sorting services to support both human and animal studies. The goals of the FCCS SRF are to provide: 1) multiparameter cell phenotyping; 2) analysis of DNA content and cell cycle; 3) analysis of cellular apoptosis and necrosis; 4) quantification of cell cytotoxicity; 5) intracellular cytokine analysis; 6) measurement of cell activation parameters; 7) high speed cell sorting of live human and animal cells under biocontainment conditions; and 8) single cell cloning of sorted cells. Another important function of the shared resource is to assist researchers in data analysis of flow cytometric studies and to help investigators in the development of new flow cytometric assays or approaches as needed for their research efforts. Finally, FCCS SRF addresses the future needs of investigators by adding new and updated instrumentation to the facility that will expand the number of techniques and approaches available to cancer researchers at the university.

Markey癌症中心（MCC）流式细胞术和细胞分选共享资源设施（FCCS SRF）为肯塔基州大学（英国）癌症研究人员提供全面、最先进的分析流式细胞术和细胞分选服务，以支持人类和动物研究。FCCS SRF的目标是提供：1）多参数细胞表型分析; 2）DNA含量和细胞周期分析; 3）细胞凋亡和坏死分析; 4）细胞毒性定量; 5）细胞内细胞因子分析; 6）细胞活化参数测量; 7）在生物防护条件下对人和动物活细胞进行高速细胞分选;和8）分选细胞的单细胞克隆。共享资源的另一个重要功能是协助研究人员进行流式细胞术研究的数据分析，并帮助研究人员开发研究工作所需的新流式细胞术检测方法或方法。最后，FCCS SRF通过向该设施添加新的和更新的仪器来满足研究人员的未来需求，这将扩大该大学癌症研究人员可用的技术和方法的数量。