PROJECT 6. Flow Cytometry

马基癌症中心(MCC)的流式细胞术和细胞分选共享资源设施(FCCS SRF)为肯塔基大学(UK)的癌症研究人员提供全面的、最先进的分析流式细胞术和细胞分选服务，以支持人类和动物研究。FCCS SRF的目标是：1)多参数细胞表型；2)DNA含量和细胞周期分析；3)细胞凋亡和坏死分析；4)细胞毒性定量；5)细胞内细胞因子分析；6)细胞激活参数测量；7)生物隔离条件下活的人和动物细胞的高速分选；8)分选细胞的单细胞克隆。共享资源的另一个重要功能是协助研究人员分析流式细胞仪研究的数据，并帮助研究人员根据其研究工作的需要开发新的流式细胞仪分析方法或方法。最后，FCCS SRF通过增加新的和更新的仪器设备来满足研究人员的未来需求，这将扩大大学癌症研究人员可用的技术和方法的数量。

The Markey Cancer Center (MCC) Flow Cytometry and Cell Sorting Shared Resource Facility (FCCS SRF) provides University of Kentucky (UK) cancer researchers with comprehensive, state-of-the-art analytical flow cytometry and cell sorting services to support both human and animal studies. The goals of the FCCS SRF are to provide: 1) multiparameter cell phenotyping; 2) analysis of DNA content and cell cycle; 3) analysis of cellular apoptosis and necrosis; 4) quantification of cell cytotoxicity; 5) intracellular cytokine analysis; 6) measurement of cell activation parameters; 7) high speed cell sorting of live human and animal cells under biocontainment conditions; and 8) single cell cloning of sorted cells. Another important function of the shared resource is to assist researchers in data analysis of flow cytometric studies and to help investigators in the development of new flow cytometric assays or approaches as needed for their research efforts. Finally, FCCS SRF addresses the future needs of investigators by adding new and updated instrumentation to the facility that will expand the number of techniques and approaches available to cancer researchers at the university.