Development of molecular markers for resistance to strawberry powdery mildew

草莓白粉病抗性分子标记的研制

• 批准号: 100875
• 金额: $ 54.45万
• 依托单位: DRISCOLL'S GENETICS LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=100875
• 关键词: Development; molecular; markers; resistance; strawberry

Strawberry powdery mildew is a serious disease problem in the UK where 80% of production is under polythene tunnels, an environment more conducive to the disease. It requires an intensive fungicide spray programme to achieve effective control and availability of suitable pesticides is decreasing. The solution is resistant varieties but breeding programmes currently select for resistance using field evaluation of symptoms. This has often resulted in short lived resistance, lasting only a few seasons before the pathogen adapts. This project will develop molecular markers to enable plant breeders to develop varieties with stronger and more durable resistance. The approach is to raise a progeny of strawberry seedlings that segregates for resistance to mildew and then develop a genetic linkage map giving good coverage of the entire strawberry genome. The map position of quantitative trait loci (QTL) associated with resistance will be located and transferable markers that are tightly linked to the resistance genes will be identified and tested in a wide range of strawberry lines to validate them and identify those most suitable for marker assisted breeding. The outcome of the project will be markers that will offer an innovative approach to resistance breeding and improve the speed, efficiency and precision, resulting in stronger and more durable resistance.

草莓白粉病在英国是一个严重的疾病问题，该国 80% 的生产是在聚乙烯隧道下进行的，这种环境更有利于该病害的发生。它需要密集的杀菌剂喷洒计划来实现有效控制，并且合适的农药的可用性正在减少。解决方案是使用抗性品种，但目前的育种计划是通过对症状进行现场评估来选择抗性。这通常会导致短暂的抵抗力，在病原体适应之前仅持续几个季节。该项目将开发分子标记，使植物育种者能够开发出具有更强、更持久抗性的品种。该方法是培育草莓幼苗的后代，这些幼苗可以进行抗霉分离，然后开发遗传连锁图谱，从而很好地覆盖整个草莓基因组。将定位与抗性相关的数量性状基因座（QTL）的图谱位置，并鉴定与抗性基因紧密连锁的可转移标记，并在广泛的草莓品系中进行测试，以对其进行验证并鉴定最适合标记辅助育种的标记。该项目的成果将是为抗性育种提供创新方法的标记，并提高速度、效率和精度，从而产生更强、更持久的抗性。