High-Throughput Platform for Thermodynamic Characterization of Biomolecular Interactions

在这个项目中，我们计划进一步开发我们的微流体液滴量热平台， 这是一个广泛的种类和大量的生物分子相互作用的表征。在证明了 技术的概念，我们计划提高系统的灵敏度和吞吐量， 对广泛的反应进行表征。该项目的目标是：1）实现多路复用的高- 单个液滴的分辨率温度读数，2）通过自动化增加系统的吞吐量 的流体处理和数据分析，以及3）通过执行演示和验证技术 各种测试样品的量热测量。综合考虑，这些改进将使 显着扩大量热法的能力，并进一步使这些能力更容易获得的 科学界。

In this project we plan to further develop our microfluidic droplet calorimetry platform to enable thermodynamic characterization of a wide variety and large number of biomolecular interactions. Having shown proof of concept of the technology, we plan to increase the sensitivity and throughput of the system to enable characterization of a broad range of reactions. The aims of the project are to 1) achieve multiplexed high- resolution temperature readout for individual droplets, 2) increase the throughput of the system via automation of the fluidic handling and data analysis, and 3) demonstrate and validate the technique by performing calorimetric measurements of a variety of test samples. Taken together, these improvements will allow for a dramatic expansion of calorimetry’s capabilities and, further, make those capabilities more accessible to the scientific community.