Core C - Biospecimans and Bioinformatics Core

项目摘要/摘要 在前一次供资期间，生物科学和生物信息学核心(核心C)在其 开发新的方法并提供必要的核心资源，用于加工、存储和 从临床研究中收集的生物样本的分布和对“2型”的生物信息学支持 炎症性表型“和基因组分析。在这一资助期间，生物科学和生物信息学 CORE将继续提供必要的加工、储存和分发从 由这笔赠款资助的临床研究，将完善质量细胞分析(CyTOF方案)并提供新的服务 与细胞质谱仪(CyTOF)相关。这些CyTOF服务包括开发和验证CyTOF 对实验标本进行面板和细胞TOF染色。核心的生物信息学资源也得到了优化 用于处理与CyTOF相关的新数据分析挑战的管道，并将提供专门的交叉 研究人员综合分析了大宗rna-seq、单细胞测序、atac-seq和DNA甲基化。 项目中提出的研究。核心C将使用来自所有3个项目的科学投入来整合这些分析 并在实验和实验组之间进行迭代分析。最后，这个核心将共享银行 样本来自IRB批准的现有UCSF呼吸道组织库，以用于本P01的项目。这个 生物信息学核心将在国家犹太人健康中心进行扩展，由Max A.Seibold领导，他也是Co-I 对于项目3。它还将有来自Matthew Spitzer博士(核心的Co-I)和专家对CyTOF的专家意见 David Erle博士(顾问/合作者)对单细胞RNA-seq分析的投入。

Project Summary/Abstract In the prior funding period, the Biospecimen and Bioinformatics Core (Core C) was extremely productive in its development of new methods and provision of an essential core resource for the processing, storage, and distribution of biospecimens collected from the clinical studies and advanced Bioinformatics support for “Type 2 inflammatory phenotyping” and genomic analyses. In this funding period, the Biospecimen and Bioinformatics Core will continue to provide essential processing, storage, and distribution of biospecimens collected from the clinical studies funded by this grant and will refine mass cytometry (CyTOF protocols) and perform new services related to mass cytometry (CyTOF). These CyTOF services include development and validation of CyTOF panels and CyTOF staining of the experimental samples. The Core’s Bioinformatics resource also has optimized pipelines for handling the new data analysis challenges related to CyTOF and will provide specialized cross- investigator integrated analyses of bulk RNA-seq, single cell sequencing, ATAC-seq and DNA methylation studies proposed in the projects. Core C will integrate these analyses using scientific input from all 3 projects and perform iterative analyses across experiments and experimental groups. Finally, this core will share banked samples from the IRB-approved existing UCSF Airway Tissue Bank to the Projects of this P01. The Bioinformatics Core will have an extension at National Jewish Health led by Max A. Seibold, who is also a Co-I for Project 3. It will also have expert input on CyTOF from Dr. Matthew Spitzer (a Co-I on the Core) and expert input on single cell RNA-seq analyses from Dr. David Erle (a consultant/collaborator).