Core 3: Mouse GBM models and Imaging Core

核心3：小鼠GBM模型和成像核心

• 批准号: 10477998
• 负责人: E. Antonio Chiocca
• 金额: $ 24.75万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-03 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10477998
• 关键词: Animals; Brain; Cells; Clinical; Collaborations; Development; Engineering; Ensure; Glioblastoma; Goals; Grant; Growth; Human Resources; Image; Immune checkpoint inhibitor; Immunology; Immunosuppression; Immunotherapy; Implant; Implantation procedure; Individual; Infection; Institution; Intracranial Neoplasms; Investigational Therapies; Luciferases; Magnetic Resonance Imaging; Maintenance; Modeling; Modification; Molecular; Monitor; Mouse Cell Line; Mus; Oncolytic; Oncolytic viruses; Operative Surgical Procedures; Performance; Quality Control; Reproducibility; Research; Research Personnel; Research Project Grants; Resistance; Resources; Sampling Biases; Service provision; Services; Signal Transduction; Simplexvirus; T-Lymphocyte; Technical Expertise; Techniques; Testing; Time; Transgenes; Tumorigenicity; United States National Institutes of Health; Validation; bioluminescence imaging; comparative; exome sequencing; experimental study; immune checkpoint; implantation; improved; interest; member; neoantigens; programs; receptor; repository; serial imaging; tumor

Core 3 Abstract To ensure reproducibility, rigor and harmonization amongst the mouse glioblastoma (GBM) models utilized by Projects 2, 3 and 4, we have established a mouse GBM model Core that is exclusively utilized for this Program Project and does not exist within participating Institutions. The Core provides broad centralized access to a central repository responsible for the maintenance and testing of mouse GBM tumors and professional expertise related to their stereotactic implantation into mouse brains and the analysis, including imaging of intracranial GBMs. It also has been providing mouse GBM neoantigen discovery and validation. This would include access to mouse cell lines and their derivatives critical to the experimental aims. This is more easily accomplished through the Core and not as easily done through individual (e.g. R01) granting mechanisms or through existing NIH sponsored service cores at the P01 institutions. This will be accomplished by pursuing two specific aims: generate and maintain a repository of mouse GBM cells, engineer GBM cells that express transgenes of interest, and identify and validate mouse GBM neoantigens (Aim 1) and provide the technical expertise and performance for the stereotactic implantation of mouse GBMs in brains and for their serial imaging (Aim 2). The provision of these services for 3 of the 4 Projects will reduce expense, will reduce technical and sample bias and will ensure that the 3 Projects will employ the same well characterized mouse GBM cells that are implanted and imaged by expert technical personnel.

核心3摘要 为了确保小鼠胶质母细胞瘤（GBM）模型的重现性、严谨性和协调性， 项目2，3和4，我们已经建立了一个小鼠GBM模型核心，专门用于本计划 项目，并不存在于参与机构。核心提供广泛的集中访问， 负责维护和检测小鼠GBM肿瘤的中心库和专业知识库 相关的立体定向植入小鼠脑内的分析，包括颅内成像 GBM。它还提供了小鼠GBM新抗原的发现和验证。这将包括进入 小鼠细胞系及其衍生物对实验目标至关重要。这是比较容易做到的 通过核心，而不是通过个人（例如R01）授予机制或通过现有的 NIH赞助P01机构的服务核心。这将通过追求两个具体目标来实现： 产生并维持小鼠GBM细胞的储存库，工程化表达感兴趣的转基因的GBM细胞， 鉴定和验证小鼠GBM新抗原（目标1），并提供技术专长和性能 用于小鼠GBM在脑中的立体定向植入及其系列成像（Aim 2）。提供 为4个项目中的3个项目提供这些服务将降低费用，减少技术和样本偏差，并确保 这3个项目将采用相同的良好表征的小鼠GBM细胞，这些细胞通过以下方式植入和成像： 专业技术人员。