P30 Center Core Grant for Vision Research

摘要 动物分析核心 视觉研究需要使用各种动物模型来研究视觉机制 发育和变性、致盲感染和炎症的发病机制以及疗效 挽救视力的疗法。大学活体动物成像和功能分析核心 俄克拉荷马州健康科学中心负责为视觉研究人员提供最先进的技术 小动物发育、退化模型的生理学和视觉功能仪器 感染、炎症。这些核心支持 NEI 资助的 16 项 R01 拨款和其他项目的愿景研究 OUHSC 的视觉相关项目位于 vivaria 内的两个不同地点，允许进出特权。更多 目前正在分析 40 多种眼病模型和 70 多种转基因小鼠品系 拥有电生理、基因分型、功能分析、显微镜和其他支持设备 专门为此用途而翻新的扩大的研究领域。该核心提供的仪器 包括视网膜电图（体内和离体）、光学相干断层扫描、眼底镜检查、光动学、 激光光凝、眼压测量、间接检眼镜检查、裂隙灯和生物显微镜成像。 环境住房还包括光损伤、黑暗饲养、缺氧/高氧、受控设备 湿度和青光眼模型。基因分型为培育视力转基因动物提供准确的数据 研究。我们的系统经理在培训人员、协助实验和数据方面非常熟练 分析并维护核心设备达到质量标准。因此，该核心整合并提供 仪器和专业知识否则对于视觉来说太昂贵或后勤上困难 校园内的研究人员可以轻松访问。与上一个资助期一样，该核心受到激励和 致力于为所有视力者提供有效、高效、人性化、高质量的研究环境 OUHSC 的研究人员。

ABSTRACT Animal Analysis Core Vision research requires the use of various animal models to investigate the mechanisms of visual development and degeneration, the pathogenesis of blinding infection and inflammation, and the efficacy of vision-saving therapeutics. The Live Animal Imaging and Functional Analysis Core at the University of Oklahoma Health Sciences Center is responsible for providing vision researchers with state-of-the-art instrumentation in the physiology and function of vision in small animal models of development, degeneration, infection, and inflammation. These Cores support vision research on 16 NEI-funded R01 grants and other vision-related projects at OUHSC in two different locations within vivaria, which allows in/out privileges. More than 40 ocular disease models and more than 70 genetically altered mouse lines are currently being analyzed with electrophysiological, genotyping, functional analysis, microscopy, and other support equipment in expanded research areas renovated specifically for this use. The instrumentation provided by this Core includes electroretinography (in vivo and ex vivo), optical coherence tomography, fundoscopy, optokinetics, laser photocoagulation, tonometry, indirect ophthalmoscopy, and slit lamp and biomicroscopic imaging. Environmental housing also includes equipment for light damage, dark rearing, hypoxia/hyperoxia, controlled humidity, and glaucoma models. Genotyping provides accurate data for breeding transgenic animals for vision research. Our Systems Managers are highly skilled in training personnel, assisting with experiments and data analysis, and maintaining core equipment to a quality standard. As such, this Core consolidates and provides instrumentation and expertise which would otherwise be too expensive or logistically difficult for vision researchers across campus to easily access. As in the previous funding period, this Core is motivated and committed to providing an effective, efficient, user-friendly, and high-quality research environment for all vision researchers at OUHSC.