Admin Supplement: Identifying the ABCs of xenobiotic metabolism in protecting the germ line lineage

联系PD/PI：施兰克尔，凯瑟琳 项目摘要/摘要 这个项目探索了原始生殖系--胚胎可用的细胞保护系统。 卵子和精子的前体。这项研究的中心是一种发育和保护功能 一类多药耐药的ABC转运蛋白，可主动外排毒素、反应性代谢物和信号 细胞中的分子。它将使用两个模型系统--海胆胚胎和人类细胞系--来 确定这些在原始生殖细胞(PGC)发育和保护中的功能。PGC还没有 尽管普遍存在“低剂量”的污染物和药物，但以前在这方面的特点是 在环境中，脐带血和母体体液。保护阈值的建模 PGC中的机制对于预测和减轻早期生命暴露至关重要，以帮助定义安全性 剂量水平和缓解子宫内可能出现的不孕不育问题。 项目摘要/摘要

Contact PD/PI: Schrankel, Catherine PROJECT SUMMARY/ABSTRACT This project explores the cellular protection systems available to the primordial germ line—the embryonic precursors to egg and sperm. The research is centered on the developmental and protective functions of a class of multi-drug resistance ABC transporters that actively efflux toxins, reactive metabolites, and signaling molecules from the cell. It will use two model systems—the sea urchin embryo and a human cell line—to identify these functions in the development and protection of primordial germ cells (PGCs). PGCs have not been characterized in this context before, despite the ubiquitous presence of “low-dose” pollutants and drugs in the environment, umbilical cord blood and maternal fluids. Modeling the threshold of protective mechanisms in PGCs is crucial for predicting and mitigating early life exposures, in order to help define safe dosage levels and mitigate infertility issues that may arise in utero. Project Summary/Abstract