Microwave-Enhanced Microreactor for Ultrafast Detection of Protein Biomarkers

用于超快速检测蛋白质生物标志物的微波增强微反应器

• 批准号: 10483753
• 负责人: Steven Ray
• 金额: $ 25.99万
• 依托单位: VIC FOUNDRY, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10483753
• 关键词: Acute; Address; Antibody Specificity; Biological; Biological Markers; Buffaloes; C-reactive protein; COVID-19 patient; COVID-19 severity; Cardiac; Characteristics; Clinical; Coupled; Coupling; Creatine Kinase; Critical Illness; Detection; Diagnosis; Digestion; Disease; Drug Targeting; Electrospray Ionization; Glial Fibrillary Acidic Protein; Goals; Heart; Heating; Immunoassay; In Situ; Infection; Inflammatory; Interleukin-6; Interleukin-8; Legal patent; Life; Mass Spectrum Analysis; Modeling; Monitor; Myocardial Infarction; Myoglobin; Patients; Peptides; Performance; Phase; Preparation; Process; Protein Analysis; Proteins; Reaction; Research; S100 Calcium Binding Protein; Sampling; Sepsis; Serum; Site; Small Business Technology Transfer Research; System; TNF gene; Technology; Testing; Therapeutic; Time; Traumatic Brain Injury; Troponin I; Trypsin; Tube; Universities; absorption; acute coronary syndrome; antibody conjugate; base; clinical diagnosis; cytokine; design; detection limit; detector; engineering design; fatty acid-binding proteins; immunoreaction; inflammatory marker; ionization; liquid chromatography mass spectrometry; magnetic beads; microwave electromagnetic radiation; nano-electrospray; photomultiplier; pilot test; procalcitonin; prognostication; protein B; protein aminoacid sequence; protein biomarkers; prototype; risk stratification; specific biomarkers; ubiquitin C-terminal hydrolase

Project Summary In diagnosis, risk stratification, and management of life-threatening diseases/conditions, every minute counts and unambiguous detection of many relevant protein biomarkers is crucial. Unfortunately, the workflow of current unambiguous approach–mass spectrometry–is bottlenecked by the sample preparation process. A microwave-enhanced, flow-through microreactor is proposed here for both selective capture and digestion of proteins to address this major limitation. The specific aims of this Phase I STTR are to create a prototype microreactor and to demonstrate a proof of concept of the microreactor-enabled mass spectrometry analysis of protein biomarkers. The proposed technology and product will streamline the workflow of mass spectrometry analysis of proteins, free end users from the laborious and tedious multiple sample preparation steps, and allow near real-time and unambiguous detection of protein biomarkers, as well as therapeutics, drug targets, etc.

项目总结