Portable Optical Imager for Multiplexed Foodborne Pathogen Detection

用于多重食源性病原体检测的便携式光学成像仪

• 批准号: 10484520
• 负责人: Zhongjian Hu
• 金额: $ 22.5万
• 依托单位: NANOHMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10484520
• 关键词: Affinity; Antibodies; Area; Bacteria; Binding; Biological Assay; Biosensing Techniques; Biosensor; Collection; Consumption; Custom; Deposition; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Electromagnetics; Engineering; Enterovirus; Enzyme-Linked Immunosorbent Assay; Epidemic; Escherichia coli O157; Feedback; Food; Food Contamination; Food Supply; Generations; Glass; Goals; Hour; Image; Immobilization; Infrastructure; Label; Laboratories; Lasers; Light; Liquid substance; Measurement; Measures; Medical; Methods; Microfabrication; Miniaturization; Monitor; Noise; Optics; Pathogen detection; Performance; Phase; Polymerase Chain Reaction; Positioning Attribute; Public Health; Rapid diagnostics; Research; Robotics; Sampling; Series; Source; Spectrum Analysis; Spottings; Staphylococcus aureus; Structure; Sucrose; Surface; Surface Properties; Swab; Techniques; Technology; Temperature; Testing; Time; Training; Uncertainty; United States Department of Agriculture; Variant; Viral; Virus; Work; aptamer; aqueous; base; clinical diagnostics; cost; cost effective; density; design; detection limit; detector; diagnostic technologies; diagnostic tool; evaluation/testing; foodborne illness; foodborne pathogen; innovation; instrumentation; lens; miniaturize; multiplex detection; optical imager; optical sensor; pathogen; plasmonics; point-of-care diagnostics; portability; prototype; rapid detection; sensor; simulation; transmission process

Portable Optical Imager for Multiplexed Foodborne Pathogen Detection Project Summary Food contamination caused by pathogens is a significant public health concern for consumers worldwide. Critical aspects of limiting foodborne disease outbreaks and epidemic involve early detection and rapid diagnostics of foodborne pathogens which require sensitive, multiplexed devices that enable point-of-care (POC) diagnostics. New generations of rapid (detection in minutes to hours), portable, multiplexed and sensitive biosensors can significantly aid in delivery of a safer food supply. Nanohmics proposes to develop a low-cost portable optical imager that is capable of fast (< 30 minutes), sensitive (tens to hundreds of bacteria or viruses), and multiplexed testing of up to tens of foodborne pathogens from fresh produce swab fluids or rinsates and comparable food surface samples. Specifically, the proposed research will integrate low-cost large-area pixelated nanohole array (NHA) biosensing chips and economical optical and electronical components in to a compact, highly portable, battery-operated and rugged biosensor device. While NHA plasmonic biosensors already exist as bench-level laboratory experimental set ups, the technology has not been innovated to make it portable and multiplexed for field use to test numerous imported or domestic foods onsite where the FDA and USDA are overwhelmed with samples. Compared to conventional reflection-type plasmonic biosensors employing a prism, NHAs support collinear excitation and collection configuration. This not only significantly simplifies alignment and but also enables small footprint and highly multiplexed biosensing. Nanohmics will microspot NHA-based pixel arrays with different bioreceptors where the type of target is encoded in the position of the corresponding spot pixels. The team will engineer and construct an optical imager using a wide-beam red laser module, a miniaturized imaging lens, and a CMOS detector, and integrate the bioreceptor-pixelated NHA biosensing chips with the optical imager for field-portable rapid multiplexed testing of infectious pathogens. The overall integrated device is envisioned to be compact (~ 5 × 5 × 10 cm3), light (~ 50 g), and low-cost (~ $ 400 including fabrication and parts). Nanohmics aims at rapid (< 30 minutes) and sensitive detection with limit of detection in the tens to low hundreds of bacteria and viruses per mL.

便携式光学成像仪在食源性致病菌检测中的应用 项目摘要 病原体造成的食品污染是世界各地消费者的重大公共卫生问题。 限制食源性疾病暴发和流行的关键方面包括及早发现和快速 食源性病原体的诊断需要能够实现护理点的灵敏、多路复用设备 (PoC)诊断。新一代快速(几分钟到几小时内检测)、便携、多路传输和 敏感的生物传感器可以显著地帮助提供更安全的食品供应。 NanoHmics计划开发一种低成本便携式光学成像仪，能够快速(&lt；30 几分钟)，敏感(数十到数百个细菌或病毒)，以及多达数十个的多路测试 来自新鲜农产品、拭子液体或糖浆以及类似食物表面的食源性病原体 样本。具体地说，拟议的研究将整合低成本的大面积像素化纳米孔 阵列(NHA)生物传感芯片和经济实惠的光学和电子元件集成到紧凑型、 高度便携、电池供电和坚固耐用的生物传感器设备。而NHA等离子体生物传感器 已经存在作为台式实验室的实验装置，该技术还没有进行创新来制造 它携带和多路复用，用于现场测试许多进口或国产食品，FDA 和美国农业部都被样品淹没了。与传统反射式等离子体生物传感器的比较 利用棱镜，NHAS支持共线激发和收集配置。这不仅是 极大地简化了对齐，而且还实现了占地面积小和高度多元化的生物传感。 NanoHMics将微点化基于NHA的像素阵列，这些像素阵列具有不同的生物受体，目标类型是 在对应的光点像素的位置中编码。该团队将设计和建造一个光学系统 使用宽束红色激光模块、微型成像透镜和cmos探测器的成像器，以及 将生物感受器像素化的NHA生物传感芯片与光学成像仪集成在一起，实现现场便携式快速检测 传染病病原体的多重检测。整个集成设备被设想为紧凑型(~5 ×5×10厘米)、重量轻(约50克)和低成本(约400美元，包括制造和部件)。NanoHmics旨在 快速(&lt；30分钟)和灵敏的检测，检测极限在几十到几百个细菌 每毫升病毒数。