Portable Optical Imager for Multiplexed Foodborne Pathogen Detection

用于多重食源性病原体检测的便携式光学成像仪

• 批准号: 10484520
• 负责人: Zhongjian Hu
• 金额: $ 22.5万
• 依托单位: NANOHMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10484520
• 关键词: Affinity; Antibodies; Area; Bacteria; Binding; Biological Assay; Biosensing Techniques; Biosensor; Collection; Consumption; Custom; Deposition; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Electromagnetics; Engineering; Enterovirus; Enzyme-Linked Immunosorbent Assay; Epidemic; Escherichia coli O157; Feedback; Food; Food Contamination; Food Supply; Generations; Glass; Goals; Hour; Image; Immobilization; Infrastructure; Label; Laboratories; Lasers; Light; Liquid substance; Measurement; Measures; Medical; Methods; Microfabrication; Miniaturization; Monitor; Noise; Optics; Pathogen detection; Performance; Phase; Polymerase Chain Reaction; Positioning Attribute; Public Health; Rapid diagnostics; Research; Robotics; Sampling; Series; Source; Spectrum Analysis; Spottings; Staphylococcus aureus; Structure; Sucrose; Surface; Surface Properties; Swab; Techniques; Technology; Temperature; Testing; Time; Training; Uncertainty; United States Department of Agriculture; Variant; Viral; Virus; Work; aptamer; aqueous; base; clinical diagnostics; cost; cost effective; density; design; detection limit; detector; diagnostic technologies; diagnostic tool; evaluation/testing; foodborne illness; foodborne pathogen; innovation; instrumentation; lens; miniaturize; multiplex detection; optical imager; optical sensor; pathogen; plasmonics; point-of-care diagnostics; portability; prototype; rapid detection; sensor; simulation; transmission process

Portable Optical Imager for Multiplexed Foodborne Pathogen Detection Project Summary Food contamination caused by pathogens is a significant public health concern for consumers worldwide. Critical aspects of limiting foodborne disease outbreaks and epidemic involve early detection and rapid diagnostics of foodborne pathogens which require sensitive, multiplexed devices that enable point-of-care (POC) diagnostics. New generations of rapid (detection in minutes to hours), portable, multiplexed and sensitive biosensors can significantly aid in delivery of a safer food supply. Nanohmics proposes to develop a low-cost portable optical imager that is capable of fast (< 30 minutes), sensitive (tens to hundreds of bacteria or viruses), and multiplexed testing of up to tens of foodborne pathogens from fresh produce swab fluids or rinsates and comparable food surface samples. Specifically, the proposed research will integrate low-cost large-area pixelated nanohole array (NHA) biosensing chips and economical optical and electronical components in to a compact, highly portable, battery-operated and rugged biosensor device. While NHA plasmonic biosensors already exist as bench-level laboratory experimental set ups, the technology has not been innovated to make it portable and multiplexed for field use to test numerous imported or domestic foods onsite where the FDA and USDA are overwhelmed with samples. Compared to conventional reflection-type plasmonic biosensors employing a prism, NHAs support collinear excitation and collection configuration. This not only significantly simplifies alignment and but also enables small footprint and highly multiplexed biosensing. Nanohmics will microspot NHA-based pixel arrays with different bioreceptors where the type of target is encoded in the position of the corresponding spot pixels. The team will engineer and construct an optical imager using a wide-beam red laser module, a miniaturized imaging lens, and a CMOS detector, and integrate the bioreceptor-pixelated NHA biosensing chips with the optical imager for field-portable rapid multiplexed testing of infectious pathogens. The overall integrated device is envisioned to be compact (~ 5 × 5 × 10 cm3), light (~ 50 g), and low-cost (~ $ 400 including fabrication and parts). Nanohmics aims at rapid (< 30 minutes) and sensitive detection with limit of detection in the tens to low hundreds of bacteria and viruses per mL.

用于多重食源性病原体检测的便携式光学成像仪 项目摘要 由病原体引起的食品污染是全球消费者关注的重大公共卫生问题。 限制食源性疾病暴发和流行的关键方面包括早期发现和快速 食源性病原体的诊断，需要敏感的多路复用设备， (POC)诊断新一代的快速（检测时间从几分钟到几小时）、便携式、多路复用和 敏感的生物传感器可以显著地帮助提供更安全的食品供应。 Nanohohmet提出开发一种低成本的便携式光学成像仪，能够快速（< 30 分钟），敏感（数十至数百种细菌或病毒），以及多达数十种 来自新鲜农产品拭子液体或冲洗液以及类似食品表面的食源性病原体 样品具体而言，拟议的研究将整合低成本大面积像素化纳米孔 阵列（NHA）生物传感芯片和经济的光学和电子组件， 高度便携、电池供电和坚固的生物传感器装置。虽然NHA等离子体生物传感器 已经存在的实验室实验台，技术还没有创新，使 它便携式和多路复用的现场使用，以测试许多进口或国内食品现场， 和美国农业部都被样品淹没了与传统的反射型等离子体生物传感器相比， 使用棱镜，NHA支持共线激发和收集配置。这不仅 显著地简化了对准，而且还实现了小覆盖区和高度多路复用的生物传感。 纳米电阻将微点基于NHA的像素阵列，具有不同的生物受体，其中目标类型是 编码在对应的点像素的位置中。该团队将设计和建造一个光学 使用宽光束红色激光器模块、小型化成像透镜和CMOS检测器的成像器，以及 将生物受体像素化的NHA生物传感芯片与光学成像仪集成， 传染性病原体的多重检测。整个集成装置被设想为紧凑的（~ 5 × 5 × 10 cm 3），重量轻（约50 g），成本低（包括制造和零件在内约400美元）。纳米技术旨在 快速（< 30分钟）和灵敏的检测，检测限为数十至数百种细菌 和病毒/mL。