Dissecting the non-growing-but-active state of a hybrid bacteria-material microdevice
剖析混合细菌材料微器件的非生长但活跃状态
基本信息
- 批准号:10487578
- 负责人:
- 金额:$ 38.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-09-15 至 2026-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressBacteriaCellsChemicalsClustered Regularly Interspaced Short Palindromic RepeatsCommunitiesDevicesDiseaseDoseDrug Delivery SystemsEcosystemEngineeringEnsureFutureGene ExpressionGenesGrowthHuman bodyHybridsMeasuresMetabolismMolecularMutationPatientsPharmaceutical PreparationsProteinsResearchSafetySiteStimulusSynthetic GenesTherapeuticWorkcommensal bacteriadrug synthesisinformation processinginterestmicrodevicemutantpreservationpreventtherapeutically effectivetool
项目摘要
PROJECT ABSTRACT
Dissecting the non-growing-but-active state of a hybrid bacteria-material microdevice
Recent work has been interested in engineering commensal bacteria to address various biomedical
challenges, such as specific drug delivery, gene-editing using CRISPR-Cas, and continuous synthesis of drugs
at disease sites. In contrast to non-living devices, bacteria can address these unmet needs because they
already live in the human body and at disease sites, move actively towards stimuli, detect and respond to
stimuli, and synthesize drugs continuously. However, a significant challenge is to prevent the uncontrolled
proliferation of bacteria, while keeping their active functions. To address the challenge, the community must
have a foolproof safety measure that prevents the replication of therapeutic bacteria in any condition. This
measure will ensure that the bacteria cannot replicate in patients and the large ecosystem. Without replication,
mutation is also rare, and mutants cannot spread. Furthermore, a non-replicating entity can be dosed more
precisely than an auto-replicating entity. Thus far, however, attempts to stop bacterial replication also
compromise the therapeutic activity of bacteria. To address this critical bottleneck, my lab has created bacteria
that cannot grow but keep high metabolism by integrating synthetic materials with bacteria. The non-growing-
but-active bacteria continue to synthesize proteins, move, and respond to chemical stimuli. My research will
further investigate the underlying mechanisms that govern the non-growing-but-active state of the bacteria.
The new understanding will then be used to boost and enable specific features of the non-growing-but-active
bacteria relevant for future biomedical applications. I will leverage my prior work about synthetic gene
expression, bacterial information processing, and CRISPR-dCas9 molecular tools. The work will reveal a new
material-protein paradigm for inhibiting growth but preserving the activity of living cells. Furthermore, the work
will enable a superior, safe, and active hybrid bacteria-material microdevice for broad biomedical applications.
项目摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Cheemeng Tan其他文献
Cheemeng Tan的其他文献
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{{ truncateString('Cheemeng Tan', 18)}}的其他基金
Dissecting the non-growing-but-active state of a hybrid bacteria-material microdevice
剖析混合细菌材料微器件的非生长但活跃状态
- 批准号:
10275397 - 财政年份:2021
- 资助金额:
$ 38.1万 - 项目类别:
Dissecting the non-growing-but-active state of a hybrid bacteria-material microdevice
剖析混合细菌材料微器件的非生长但活跃状态
- 批准号:
10670969 - 财政年份:2021
- 资助金额:
$ 38.1万 - 项目类别:
Dissecting the non-growing-but-active state of a hybrid bacteria-material microdevice
剖析混合细菌材料微器件的非生长但活跃状态
- 批准号:
10797951 - 财政年份:2021
- 资助金额:
$ 38.1万 - 项目类别:
Modeling heterogeneity of a cancer-signaling cascade using biomimetic cells
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9886240 - 财政年份:2019
- 资助金额:
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