P30-Core Grant for Vision Research

总体摘要 要求继续为NEI视力研究核心补助金提供支持，以支持 亚特兰大视力研究社区(AVRC)。本中心奖助金续期申请包括三项 资源/服务核心：(1)结构生物学和成像，(2)功能基因组学和蛋白质组学，以及(3) 生物信息学和生物统计学。AVRC核心赠款支持协作视觉研究和服务 在23名教职员工中，18名符合条件的NEI资助了R01，24名博士后研究员和38名博士后研究员。这 支持主要来自AVRC人员的专业知识和服务，他们对AVRC教职员工的培训， 以及设备、用品和其他资源的可用性。在过去的5年里，核心赠款贡献了 为193份同行评议的出版物和许多由NEI资助的新的和竞争对手的续订提供了资金 R01S。在接下来的5年里，我们正在积极招聘5-7名初级研究终身教员，他们将重新- 振兴AVRC和欧洲经济共同体，增加这项P30核心拨款的需求、价值和科学影响。 结构生物学和成像(SBI)核心为AVRC成员提供资源和服务 关于确定健康人眼结构正常与异常关系的探讨 在有和没有实验疗法的疾病模型中。为结构提供支撑-- 功能研究，重点是视网膜功能、视网膜变性/损伤、RPE形态测量、眼部 肿瘤学和基因/药物输送。核心支持超声检查、免疫组织化学、共聚焦 显微镜、荧光图像分析以及光学和电子显微镜服务。 功能基因组学和蛋白质组学(FG&P)核心的目标是加强研究，以增加我们的 对生物功能(例如，视觉)如何从生物体的 基因组，并通过表观遗传学进行修改。核心支持分子、生化和表型 实验分为六个部分：DNA/RNA服务、蛋白质/小分子分析、细胞培养和 眼/视觉结构和功能分析、啮齿动物眼部显微外科手术和小鼠群体服务。 方法包括扫描激光眼底检查、SD-OCT、眼底成像、精密显微测量、 光折射、回弹眼压、ERG、OKT、高效液相、LC-MS、PCR、LCM、免疫印迹和ELISA法、Flow- 细胞学、蛋白质测序、微阵列分析和NexGen测序。 生物信息学和生物统计(生物分析)核心提供生物统计、生物信息学、数据管理、 电子笔记本、数据备份和高级计算分析。支持包括以下努力 核心-专职的信息学家和统计学家、高级软件和云计算访问。 咨询委员会协助核心董事确保充分和公平地使用设施，并就 根据需要创建新资源或淘汰未充分利用的组件。这些核心有 提高并将继续通过提供共同服务来刺激和提高研究生产率 促进教师之间的合作研究，并吸引其他学术学科参与愿景研究。

Overall Summary Continued support is requested for the NEI Core Grant for Vision Research that supports investigators of the Atlanta Vision Research Community (AVRC). This Center Grant renewal application includes three Resource/Service Cores: (1) Structural Biology and Imaging, (2) Functional Genomics and Proteomics, and (3) Bioinformatics and Biostatistics. The AVRC Core grant supports collaborative vision research and service among 23 faculty with 18 eligible NEI funded R01s, 24 postdoctoral fellows, and 38 predoctoral fellows. This support comes largely via the expertise and service of AVRC personnel, their training of AVRC faculty and staff, and the availability of equipment, supplies, and other resources. In the past 5 years, the Core grant contributed to the generation of 193 peer-reviewed publications and to many new and competing renewal NEI-funded R01s. During the next 5 years, we are actively recruiting 5-7 junior research tenure-track faculty who will re- invigorate the AVRC and the EEC, increasing the need, value, and scientific impact of this P30 Core grant. The Structural Biology and Imaging (SBI) Core provides resources and services for members of the AVRC with regard to determining the relationship between normal and abnormal structure of ocular structures in healthy tissue and in disease models with and without experimental therapies. Support is provided for structure- function studies with an emphasis on retinal function, retinal degeneration/injury, RPE morphometry, ocular oncology and gene/drug delivery. The Core supports ultrasonography, immunohistochemistry, confocal microscopy, fluorescence image analysis, and light and electron microscopy services. The goal of the Functional Genomics and Proteomics (FG&P) Core is to enhance research that increases our understanding of how biological function (e.g., vision) arises from the information encoded in an organism's genome and is modified by epigenetics. The Core supports molecular, biochemical, and phenotypic experiments via six sections: DNA/RNA services, protein/small molecule analysis, cell culture, and ocular/vision structural and functional analysis, rodent ocular microsurgery, and mouse colony services. Approaches include scanning laser ophthalmoscopy, SD-OCT, fundus imaging, precision micrometry, photorefraction, rebound tonometry, ERG, OKT, HPLC, LC-MS, PCR, LCM, immunoblotting and ELISA, flow- cytometry, protein sequencing, microarray analysis, and NexGen sequencing. The Bioinformatics and Biostatistics (Bioanalysis) Core provides biostatistics, bioinformatics, data management, electronic notebooks, data backups, and advanced computational analyses. The support includes effort of Core-dedicated informaticists and statisticians, advanced software, and cloud-computing access. An Advisory Committee aids the Core directors to insure full and equitable use of the facilities and to advise on creation of new resources based on need or elimination of components that are underutilized. The Cores have increased and will continue to increase research productivity by providing common services to stimulate and facilitate collaborative studies among faculty and to attract other academic disciplines to vision research.