Synthetic Biology and Optogenetics Core

合成生物学和光遗传学核心

• 批准号: 10491052
• 负责人: ANDREI V KARGINOV
• 金额: $ 31.88万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-20 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10491052
• 关键词: Animal Model; Avena sativa; Biology; CREB1 gene; Cell model; Chemicals; Chimera organism; Complex; Custom; Cyclic AMP-Dependent Protein Kinases; Development; Engineering; Enterobacteria phage P1 Cre recombinase; Enzymes; G-Protein-Coupled Receptors; Goals; Heterodimerization; Individual; Inflammatory; JAK1 gene; Ligands; Light; Location; Lung; Mediating; Methods; Molecular; Neurospora crassa; Nuclear; Participant; Pathway interactions; Phosphorylation; Phosphotransferases; Photoreceptors; Process; Productivity; Protein Engineering; Protein Kinase; Proteins; Protocols documentation; Pulmonary Inflammation; Pyruvate Dehydrogenase Complex; Reagent; Regulation; Reporting; Resolution; Rhodopsin; Role; STAT1 gene; STAT6 gene; Signal Pathway; Signal Transduction; Sirolimus; System; Technology; Transcriptional Activation; analog; calmodulin-dependent protein kinase II; dimer; extracellular; improved; in vivo; interest; lung injury; macrophage; mouse model; novel; optogenetics; phototropin; programs; protein activation; protein kinase A kinase; receptor; reconstruction; synthetic biology; tool; transcription factor; wireless

Abstract The Program Project will employ in all projects novel engineered reagents that enable manipulation and interrogation of individual signaling pathways with precise spatial and temporal control. As described in individual Projects, reagents provided by Core B will be used to define the signaling processes that regulate plasticity and specialization of macrophages and define their role during lung inflammation and injury as well as resolution. Development and optimization of these tools will require the efforts of the Synthetic Biology and Optogenetics Core B. The central functions of Core B will be 1) to develop molecular tools customized for each specific question in individual Projects, 2) to evaluate the new reagents as they come on-line and establish protocols for their application in macrophages and in in vivo mouse model, and 3) to provide assistance to all Projects with the application of the tools, troubleshooting, and analysis of the results. The intent will be to simplify application of these advances technologies to dissect specific signaling pathways in the Program Project and allow participants to focus on the proposed questions requiring these reagents. The specific tools that will be developed and employed by Core B include: 1) new reagents to control localization and interactions of proteins identified by Projects; and 2) reagents for regulation of activity of selected proteins in living macrophages, and targeted activation of these proteins in specific complexes and subcellular locations. Core B will also generate and optimize reagents for expression of engineered proteins in macrophages and develop new reagents as needed for manipulation and interrogation of protein interactions and cell signaling. The tools developed will enable manipulation of individual signaling pathways, deconstruction of these pathways, and assessment of their roles in the regulating function of macrophages in lungs.

摘要 该计划项目将在所有项目中采用新的工程试剂，使操作和 通过精确的空间和时间控制来询问个体信号通路。如个别 项目，核心B提供的试剂将用于定义调节可塑性的信号传导过程， 巨噬细胞的特化，并确定其在肺部炎症和损伤以及解决过程中的作用。 这些工具的开发和优化将需要合成生物学和光遗传学的努力。 核心B。核心B的中心功能是：1）开发针对每个特定 2）在新试剂上线时对其进行评估，并建立 它们在巨噬细胞和体内小鼠模型中的应用，以及3）为所有项目提供帮助， 工具的应用、故障排除和结果分析。目的是简化应用程序 这些先进的技术，剖析特定的信号通路的计划项目，并允许 请与会者集中讨论需要这些试剂的拟议问题。将开发的具体工具 核心B采用的新试剂包括：1）控制已鉴定蛋白质的定位和相互作用的新试剂 2）用于调节活巨噬细胞中选定蛋白质活性的试剂， 这些蛋白质在特定复合物和亚细胞位置的激活。核心B还将生成和 优化用于在巨噬细胞中表达工程蛋白的试剂，并根据需要开发新试剂 用于操纵和询问蛋白质相互作用和细胞信号。开发的工具将使 操纵个别信号通路，解构这些通路，并评估其作用 调节肺内巨噬细胞的功能。