权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Autophagy-facilitated secretion in metabolic maintenance

自噬促进代谢维持中的分泌

基本信息

批准号：
10490364
负责人：
Congcong He
金额：
$ 40万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-17 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10490364
关键词：
5&apos -AMP-activated protein kinase Adipocytes Adipose tissue Attention Autophagocytosis Binding Biochemical Biochemistry Blood Circulation Cellular biology Complex Cyclic AMP-Dependent Protein Kinases Data Endocrine Glands Endoplasmic Reticulum Genes Genetic Goals High Fat Diet Homeostasis Hormonal Hormones Human Hyperactivity Image Insulin Resistance Knock-in Mouse Knockout Mice Link LoxP-flanked allele Lysosomes Maintenance Mediating Metabolic Metabolic dysfunction Metabolic syndrome Metabolism Molecular Mus Mutant Strains Mice Mutation Non-Insulin-Dependent Diabetes Mellitus Pathway interactions Pharmacology Phenocopy Phenotype Phosphotransferases Play Process Proteins Proteomics Receptor Signaling Regulation Research Role Serum Signal Transduction System Testing Tissues Vesicle adipokines adiponectin feeding glucose tolerance improved insulin sensitivity insulin sensitizing drugs mouse model novel prevent response tool

项目摘要

Project Summary/Abstract The goal of this proposal is to demonstrate a novel non-degradative, non-cell autonomous, mechanism by which the autophagy machinery regulates metabolism. Although emerging evidence suggests that autophagy abnormality is implicated in metabolic dysfunction, the mechanism by which autophagy regulates insulin sensitivity is largely unknown. Autophagy is generally considered as a destruction and degradation process via lysosomes. We recently discovered a new non-degradative function regulated by a ULK1-Vps34-Becn1 autophagy machinery in adipose tissue, which may play a pivotal role in systemic AMPK activation and insulin sensitization. Adiponectin is an adipose-derived hormone (adipokine), whose reduction in circulation is strongly associated with type 2 diabetes (T2D) and metabolic syndrome. Hypoadiponectinemia caused by SNPs or mutations in the Adiponectin gene in humans has been associated with insulin resistance and T2D, and adiponectin knockout mice phenocopy the human phenotypes and develop insulin resistance. However, despite the functional importance of adiponectin, the mechanism that regulates adiponectin secretion has received little research attention and remains obscure. We found that autophagy-hyperactive mutant mice harboring an active mutation in the essential autophagy protein Becn1 (Becn1F121A knock-in mice) have improved insulin sensitivity in response to high-fat diet feeding, which is caused by factors in the circulation. To identify the Becn1-regulated, insulin-sensitizing, circulating factors, we performed a hormonal screen and identified a higher level of adiponectin in the serum of autophagy-hyperactive Becn1F121A mice. We found that on one hand, adipose- specific Becn1F121A expression is sufficient to improve systemic insulin sensitivity and increase circulating adiponectin levels; on the other hand, depleting Becn1, or inhibiting two upstream autophagy kinases, ULK1 and Vps34, reduces circulating adiponectin in mice. Using proteomic and biochemical approaches, we discovered that the exocyst component Sec6/Exoc3 is a binding partner of Becn1, and preferentially binds to the hyperactive form, Becn1F121A. Motivated by the preliminary data, we propose our overall hypothesis that a ULK1-Vps34- Becn1 autophagy axis improves insulin sensitivity by promoting adiponectin secretion via Becn1-exocyst binding in adipocytes. We term this pathway as “autophagy-facilitated secretion”. To test the hypothesis, we propose the following aims, using a combination of genetic, imaging, cell biology, biochemistry, and metabolic approaches: Aim 1 is to determine whether active Becn1 activates AMPK and improves insulin sensitivity via adiponectin signaling; Aim 2 is to determine whether a ULK1-Vps34-Becn1 autophagy axis functions in adipose tissue to non-cell autonomously regulate systemic insulin sensitivity; and Aim 3 is to demonstrate the molecular mechanism by which the Becn1-centered autophagy machinery regulates adiponectin secretion via the crosstalk with the exocyst pathway. Overall, our study will establish a new mechanistic paradigm for the Becn1-centered autophagy pathway in adipose tissue to prevent T2D, beyond its role in self-degradation.

项目总结/文摘