Molecular Profiling (MP) Core G

摘要/摘要：分子分析核心G 分子分析核心(MP)核心G的目标是：(A)履行研究范围内的职责 生物库包括根据需要跟踪样本并将其分发给项目3和其他核心和项目， (B)促进通过NCRAD(国家中央档案库)迅速公开共享生物检疫品 阿尔茨海默病和相关痴呆症)，每年向他们提供两次样品发货 向一般科学界提供沉积和管理，(C)生成分子数据 从项目1和项目2收集的生物样品(D)提供对基因组数据的迭代重新分析(RNA和 DNA)，以确保有关以下方面的所有研究数据都是最新的 它们各自的分析和注释管道，以及(E)就以下各方面提供专家咨询 生物标记物的发现和发展给精密老化网络(PAN)的研究人员。 项目1将由10,000张干血斑卡片(DBS)组成。这些DBS样本是自助式的 从全国分布的参与MindCrowd-Expanded的个人队列收集 (MC-扩展)队列。MP Core将对这些标本进行APOE和基于阵列的基因分型 通过数据科学(DS)核心将原始和处理后的数据存入研究数据储存库。这些 数据将被项目1调查人员用来确定与或 修改，在MC扩展测试电池上的认知性能。此外，通过使用基因组- 广泛的数据，每个参与者将被分配到与以下方面有关的个性化多基因风险评分(PR 项目2中详述的各种风险因素(如阿尔茨海默病、高血压、糖尿病、睡眠等)。 项目2生物标本将包括从面对面收集的1,620份血浆、血清和脑脊液样本 具有特征的队列。将从这些样本中生成几个分子图谱数据集，包括； 全基因组测序，脑脊液生物标记物，免疫和炎症测量，泛病毒暴露， T和B细胞受体谱系，全血和无细胞外切体RNA测序，以及Horvath 表观遗传时钟决定等(在MP核心中进一步详细说明)。这些数据将是高质量的 以原始格式和处理格式控制并上载到研究数据存储库，以供 项目和岩心的调查人员。 所获得的高质量和广泛的分子数据将在回答主要问题时发挥重要作用 在我们提议的项目中提出的研究问题，也可以被国家研究界使用 研究认知老化、阿尔茨海默病(AD)和相关痴呆(ADRD)。

SUMMARY/ABSTRACT: Molecular Profiling Core G The goals of the Molecular Profiling Core (MP) Core G are to (a) perform the duties of the study-wide biobank including tracking and distribution of samples to Project 3 and other Cores and Projects as needed, (b) facilitate the rapid public sharing of biospecimens through NCRAD (National Centralized Repository for Alzheimer’s Disease and Related Dementias) by providing them with twice yearly sample shipments for deposition and administration to the general scientific community, (c) generate molecular data for biospecimens collected from Projects 1 and 2, (d) provide iterative re-analysis of genomic data (RNA and DNA) as necessitated by the activities of Project 4 to ensure that all study data is up-to-date with regards to their respective analytical and annotation pipelines, and (e) provide expert consultation on all aspects of biomarker discovery and development to the Precision Aging Network (PAN) investigators. Project 1 biospecimens will consist of 10,000 dried blood spot cards (DBS). These DBS samples are self- collected from the nationally distributed cohort of individuals who participate in the MindCrowd-Expanded (MC-Expanded) cohort. The MP Core will perform APOE and array-based genotyping on these specimens and deposit the raw and processed data into the study data repository via the Data Science (DS) Core. These data will be utilized by Project 1 investigators to identify genetic risk factors that are associated with, or that modify, cognitive performance on the MC-Expanded test battery. Additionally, through the use of the genome- wide array data, each participant will be assigned individualized polygenic risk score (PRS) pertaining to the various risk factors detailed in Project 2 (e.g., Alzheimer’s disease, hypertension, diabetes, sleep, etc.). Project 2 biospecimens will consist of 1,620 plasma, serum, and CSF samples collected from the face-to-face characterized cohort. Several molecular profiling datasets will be generated from these samples including; whole genome sequencing, CSF biomarkers, immune and inflammatory measurements, pan-viral exposures, T- and B-cell receptor repertoires, whole blood and cell-free exosome RNA sequencing, and Horvath epigenetic clock determinations among others (detailed further in the MP Core). These data will be quality controlled and uploaded to the study data repository in both raw and processed formats for use by investigators in the Projects and Cores. The acquired high-quality and wide-ranging molecular data will play significant roles in answering major research questions in our proposed projects, and can also be used by the national research community studying cognitive aging, Alzheimer’s disease (AD), and related dementias (ADRD).