MHC class II immunopeptidomics analysis of the SARS-CoV-2 proteome

研究项目总结 新冠肺炎是一场全球性的流行病，其范围之广是百年未见的，而且有一个严重的未得到满足的 需要一种疫苗来提供对SARS-CoV-2的群体免疫力。这项提案的目标是确定 SARS-CoV-2蛋白质组中可能被整合的保守的免疫优势MHC II类多肽 到目前的疫苗设计研究中。这满足了设计一种长期有效的疫苗的迫切需要。 对新冠肺炎的保护性免疫。 这项提案使用创新的免疫营养学平台来定义人类SARS-CoV-2 MHC II类 用串联质谱仪(AIM 1)检测免疫表位，并测定CD4+T细胞对 使用人源化小鼠模型的潜在免疫原性SARS-CoV-2 MHC-II多肽(目标2)。强健的CD4+ T细胞对病毒多肽的反应对于建立长寿记忆B细胞池是必不可少的，并具有细胞毒性 T细胞。这项研究的结果将为了解CD4+T细胞对病毒蛋白的反应提供有价值的见解 可能会迅速被纳入疫苗设计，提供针对新冠肺炎的长期保护。

SUMMARY OF RESEARCH PROJECT COVID-19 is a global pandemic, the scope of which has not been seen in a century, and there is a critical unmet need for a vaccine to provide herd immunity against SARS-CoV-2. The objective of this proposal is to identify conserved immunodominant MHC class II peptides from the SARS-CoV-2 proteome that may be incorporated into current vaccine design studies. This meets a critical need to design a vaccine that will provide long-term protective immunity against COVID-19. This proposal uses an innovative immunopeptidomics platform to define the human SARS-CoV-2 MHC class II immunopeptidome using tandem mass spectrometry (Aim 1), and to determine CD4+ T cell responses to potentially immunogenic SARS-CoV-2 MHC-II peptides using a humanized mouse model (Aim 2). Robust CD4+ T cell responses to viral peptides are essential for establishing a pool of long-lived memory B cells and cytotoxic T cells. Results from this study will provide valuable insights into CD4+ T cell responses to viral proteins that may be rapidly incorporated into a vaccine design that will provide long-lasting protection from COVID-19.