Core B Bioinformatics Core

核心 B 生物信息学核心

• 批准号: 10494656
• 负责人: Shannan J Ho Sui
• 金额: $ 19.44万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-30 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10494656
• 关键词: ATAC-seq; Achievement; Address; Bioinformatics; Biological Models; Chromatin; Clinical; Computer software; DNA Methylation; Data; Data Analyses; Data Management Resources; Data Set; Deposition; Epigenetic Process; Event; Experimental Designs; Experimental Models; Formulation; Functional disorder; Gene Expression; Genes; Genomic Segment; Goals; Infrastructure; Intuition; Investigation; Link; Maps; Metabolic; Methods; Molecular; Online Systems; Organism; Pathway interactions; Public Health Schools; Quality Control; Receptor Cell; Regulatory Pathway; Research; Research Personnel; Resources; Retrieval; Role; Sampling; Signal Transduction; Skin; Skin wound healing; Software Engineering; Supervision; System; Testing; Visualization; Visualization software; Work; age related; base; bioinformatics pipeline; data exploration; design; experimental study; insight; member; multidisciplinary; multiple omics; next generation sequencing; novel; programs; skin regeneration; statistics; stem cells; transcriptome sequencing; transcriptomics; web portal; wound healing

SUMMARY The Core B. Bioinformatics Core will serve as a comprehensive, multi-disciplinary resource of expertise in bioinformatics and statistics. It will support the data analysis needs of the program project, collaborating closely with investigators in the design, management, analysis and exploration of their high throughput omics experiments. Core B will assist investigators in addressing their aims at all levels to provide insights into age- related skin regeneration and wound healing through bioinformatics inference. The transcriptomics, DNA methylation and chromatin accessibility data generated through this program project will be consistently analyzed using state-of-the-art methods for next generation sequencing analysis implemented in Core B’s infrastructure that provide rigorous quality control, mapping, and quantitation of genes and genomic regions with functional roles in skin regeneration. Using established unsupervised and supervised multi-omics integrative approaches, Core B will identify shared signals across experiments and work with program investigators to map key metabolic, epigenetic and cell receptor stem cell pathways that drive age-related wound healing dysfunction. The results of these analyses will be made available through an interactive web based visualization system for storage, retrieval and exploration of the data generated by the program projects, enabling the achievement of the project aims and facilitating formulation of new testable hypotheses beyond those stated.

总结 核心B。生物信息学核心将作为一个全面的，多学科的专业知识资源， 生物信息学和统计学。它将支持项目的数据分析需求，密切合作， 与研究人员一起设计、管理、分析和探索他们的高通量组学 实验核心B将协助研究者在各个层面实现其目标，以提供对年龄的深入了解- 相关的皮肤再生和伤口愈合通过生物信息学推理。转录组学，DNA 通过这个项目产生的甲基化和染色质可及性数据将得到一致的分析 在Core B的基础设施中使用最先进的方法进行下一代测序分析 这些技术提供了严格的质量控制、作图和基因和基因组区域的定量， 在皮肤再生中的作用使用已建立的无监督和有监督的多组学综合方法， 核心B将识别实验中的共享信号，并与项目调查人员合作绘制关键代谢图， 表观遗传和细胞受体干细胞途径驱动年龄相关的伤口愈合功能障碍。的结果 这些分析将通过一个基于交互式网络的可视化系统提供， 和探索由计划项目产生的数据，使项目目标的实现， 促进形成新的可检验的假设，而不是那些陈述的假设。