Core B Bioinformatics Core

核心 B 生物信息学核心

• 批准号: 10494656
• 负责人: Shannan J Ho Sui
• 金额: $ 19.44万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-30 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10494656
• 关键词: ATAC-seq; Achievement; Address; Bioinformatics; Biological Models; Chromatin; Clinical; Computer software; DNA Methylation; Data; Data Analyses; Data Management Resources; Data Set; Deposition; Epigenetic Process; Event; Experimental Designs; Experimental Models; Formulation; Functional disorder; Gene Expression; Genes; Genomic Segment; Goals; Infrastructure; Intuition; Investigation; Link; Maps; Metabolic; Methods; Molecular; Online Systems; Organism; Pathway interactions; Public Health Schools; Quality Control; Receptor Cell; Regulatory Pathway; Research; Research Personnel; Resources; Retrieval; Role; Sampling; Signal Transduction; Skin; Skin wound healing; Software Engineering; Supervision; System; Testing; Visualization; Visualization software; Work; age related; base; bioinformatics pipeline; data exploration; design; experimental study; insight; member; multidisciplinary; multiple omics; next generation sequencing; novel; programs; skin regeneration; statistics; stem cells; transcriptome sequencing; transcriptomics; web portal; wound healing

SUMMARY The Core B. Bioinformatics Core will serve as a comprehensive, multi-disciplinary resource of expertise in bioinformatics and statistics. It will support the data analysis needs of the program project, collaborating closely with investigators in the design, management, analysis and exploration of their high throughput omics experiments. Core B will assist investigators in addressing their aims at all levels to provide insights into age- related skin regeneration and wound healing through bioinformatics inference. The transcriptomics, DNA methylation and chromatin accessibility data generated through this program project will be consistently analyzed using state-of-the-art methods for next generation sequencing analysis implemented in Core B’s infrastructure that provide rigorous quality control, mapping, and quantitation of genes and genomic regions with functional roles in skin regeneration. Using established unsupervised and supervised multi-omics integrative approaches, Core B will identify shared signals across experiments and work with program investigators to map key metabolic, epigenetic and cell receptor stem cell pathways that drive age-related wound healing dysfunction. The results of these analyses will be made available through an interactive web based visualization system for storage, retrieval and exploration of the data generated by the program projects, enabling the achievement of the project aims and facilitating formulation of new testable hypotheses beyond those stated.

概括 核心B.生物信息学核心将成为一种全面的，多学科的专业资源 生物信息学和统计。它将支持该计划项目的数据分析需求，并密切合作 随着设计，管理，分析和探索其高吞吐量OMICS的研究人员 实验。核心B将协助调查人员解决他们在各个层面的目标，以提供对年龄的见解 - 通过生物信息学推断，相关的皮肤再生和伤口愈合。转录组学，DNA 通过此程序项目生成的甲基化和染色质可及性数据将一致分析 使用最先进的方法用于在Core B的基础架构中实现的下一代测序分析 提供了具有功能性的基因和基因组区域的严格质量控制，映射和定量 在皮肤再生中的作用。使用已建立的无监督和监督的多摩斯综合方法， 核心B将确定跨实验的共享信号，并与计划调查人员合作以绘制关键代谢， 表观遗传和细胞受体干细胞途径，驱动与年龄相关的伤口愈合功能障碍。结果 这些分析将通过基于交互式Web的可视化系统进行存储，检索 以及对计划项目产生的数据的探索，实现了项目的目标和 促进新的可检验假设的公式以外的假设。