Genetic Engineering Core

基因工程核心

基本信息

项目摘要

PROJECT SUMMARY GENETIC ENGINEERING CORE (GEC) The overall goal of the Genetic Engineering Core (GEC) is to support the needs of MARMO-AD by providing services to (1) genetically engineer marmosets using CRISPR/Cas9 gene editing, (2) develop and optimize enabling technologies for germline gene editing in marmoset, and (3) create fibroblast cell lines and differentiate them into induce neuronal stem cells and neurons. The common marmoset (Callithrix jacchus) is a New World non-human primate with numerous practical advantages in biomedical research and is quickly becoming a much sought-after model organism by the neuroscience community. The rapidly growing appreciation of marmoset neurobiology is synergizing with contemporaneous advances in gene editing techniques to make marmosets the genetically engineered non-human primate of choice for understanding brain function and to study brain disorders such as Alzheimer’s Disease. The GEC will provide all services required to use CRISPR/Cas9 gene editing to produce novel genetically engineered marmosets that harbor human high-risk alleles for early and late onset Alzheimer’s disease. While creating these gene-edited animals, the GEC will simultaneously develop and optimize enabling technologies for efficient germline gene editing. These technologies include novel methods of ovarian stimulation to enhance production of mature marmoset oocytes, and novel approaches to reduce mosaicism in gene edited marmosets. Improved technologies will reduce the cost and increase the speed at which genetically engineered marmosets can be produced while also reducing animal numbers. Lastly, the GEC will produce marmoset fibroblast cell lines and differentiate them into induced neuronal stem cells and neurons. Cell lines will be used to create DNA sequencing libraries and for in vitro phenotypic characterization of gene edited animals in support of the Project goals.
基因工程核心(gec)

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Gregg E. Homanics其他文献

The PDE4 inhibitor apremilast modulates ethanol responses in emGabrb1/em-S409A knock-in mice via PKA-dependent and independent mechanisms
PDE4抑制剂Apremilast通过PKA依赖和独立机制调节EMGABRB1/EM-S409A敲入小鼠中的乙醇反应
  • DOI:
    10.1016/j.neuropharm.2024.110035
  • 发表时间:
    2024-10-01
  • 期刊:
  • 影响因子:
    4.600
  • 作者:
    Yuri A. Blednov;William Shawlot;Gregg E. Homanics;Elizabeth A. Osterndorff-Kahanek;Sonia Mason;Jody Mayfield;Joshua L. Smalley;Stephen J. Moss;Robert O. Messing
  • 通讯作者:
    Robert O. Messing

Gregg E. Homanics的其他文献

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{{ truncateString('Gregg E. Homanics', 18)}}的其他基金

Ethanol Mechanisms in GABAAR Gene Targeted Mice
GABAAR 基因靶向小鼠的乙醇机制
  • 批准号:
    8839369
  • 财政年份:
    2015
  • 资助金额:
    $ 35.15万
  • 项目类别:
Genetically Engineered Rodent Care
基因工程啮齿动物护理
  • 批准号:
    8531079
  • 财政年份:
    2011
  • 资助金额:
    $ 35.15万
  • 项目类别:
Genetically Engineered Rodent Care
基因工程啮齿动物护理
  • 批准号:
    8718952
  • 财政年份:
    2011
  • 资助金额:
    $ 35.15万
  • 项目类别:
Genetically Engineered Rodent Care
基因工程啮齿动物护理
  • 批准号:
    8230147
  • 财政年份:
    2011
  • 资助金额:
    $ 35.15万
  • 项目类别:
Genetically Engineered Rodent Care
基因工程啮齿动物护理
  • 批准号:
    9324404
  • 财政年份:
    2011
  • 资助金额:
    $ 35.15万
  • 项目类别:
Role of noncoding RNA in alcohol action
非编码RNA在酒精作用中的作用
  • 批准号:
    9240749
  • 财政年份:
    2011
  • 资助金额:
    $ 35.15万
  • 项目类别:
Genetically Engineered Rodent Care
基因工程啮齿动物护理
  • 批准号:
    8327743
  • 财政年份:
    2011
  • 资助金额:
    $ 35.15万
  • 项目类别:
G Protein Modulation of Glycine Receptor Function and Ethanol Action
G 蛋白对甘氨酸受体功能和乙醇作用的调节
  • 批准号:
    7893837
  • 财政年份:
    2009
  • 资助金额:
    $ 35.15万
  • 项目类别:
G Protein Modulation of Glycine Receptor Function and Ethanol Action
G 蛋白对甘氨酸受体功能和乙醇作用的调节
  • 批准号:
    8320784
  • 财政年份:
    2008
  • 资助金额:
    $ 35.15万
  • 项目类别:
G Protein Modulation of Glycine Receptor Function and Ethanol Action
G 蛋白对甘氨酸受体功能和乙醇作用的调节
  • 批准号:
    7921500
  • 财政年份:
    2008
  • 资助金额:
    $ 35.15万
  • 项目类别:
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