Research Services Core

研究服务核心

• 批准号: 10495209
• 负责人: Adriana Galvan
• 金额: $ 29.12万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-29 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10495209
• 关键词: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; Acute; Anatomy; Animal Model; Animals; Antiparkinson Agents; Behavior; Behavior assessment; Behavioral; Bioinformatics; Biometry; Brain; Caring; Cells; Chronic; Clinical; Conceptions; Consultations; Data; Data Analyses; Denervation; Dopamine; Dose; Electrodes; Electrophysiology (science); Evaluation; Experimental Designs; Feedback; Fiber Optics; Genes; Health Services Accessibility; Histologic; Histological Techniques; Histology; Human Resources; Image Analysis; Immunohistochemistry; Injections; Levodopa; Location; MPTP model; MPTP treatment; Macaca mulatta; Methods; Microscopy; Modeling; Monkeys; Mus; Neurotoxins; Opsin; Oxidopamine; Parkinson Disease; Parkinsonian Disorders; Pattern; Penetration; Procedures; Process; Proteins; Public Health Schools; Reproducibility; Research; Research Design; Research Personnel; Research Project Grants; Research Project Summaries; Resources; Rhesus; Risk; Services; Spinal; Standardization; Statistical Data Interpretation; Techniques; Testing; Time; Tracer; Transgenes; Universities; Viral Vector; Virus; biocytin; brain tissue; data sharing; design; digital imaging; experience; experimental analysis; experimental study; instructor; light microscopy; nervous system disorder; optogenetics; parkinsonian animal; parkinsonian non-human primate; professor; reconstruction; sensor; statistical service; tissue processing; transgene expression; voltage

Project Summary – Research Services Core (Core B) The Research Services Core (Core B) will be an essential part of the Emory Udall Center. The Core will have three functions: (1) to prepare and process the brain tissue from experimental animals generated by the Center’s projects, (2) to treat Rhesus monkeys systemically with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and evaluate the resulting parkinsonism in a standardized manner, and (3) to provide biostatistical services and consultation for all projects in the Center. The Core will provide standardized histology services to researchers using animal models of parkinsonism (projects 1-3). These services will include the use of immunohistochemical and microscopy approaches to evaluate the degree of dopamine depletion in 6-hydroxydopamine (6-OHDA) or MPTP-treated animals (projects 1-3); histological reconstructions of recording electrode trajectories (projects 1 and 2); analysis of the degree and pattern of expression of transgenes (opsins, fluorescent proteins or voltage sensors) after virus injections (projects 1-3). These services will be indispensable to interpret the functional experiments and will help to compare and share the results among the projects. Similarly, all MPTP-treated parkinsonian monkeys that will be used in projects 2 and 3 will be treated and evaluated using standardized procedures by Core B personnel. This will help to integrate and compare data generated from these projects. Finally, the Core will provide the services of a biostatistician that will advise and provide feedback on statistical aspects of the projects. All four projects in the Udall Center will have access to these services, from the conception and design of the experiments to the statistical analysis of experimental data. By providing these services, the Core will help to free up time and resources from the research projects. In addition, center research will benefit from the use of standardized, well-establish anatomical and behavioral assessment techniques, and the biostatistical support will promote rigor and reproducibility in all projects.

项目摘要-研究服务核心(核心B) 研究服务核心(核心B)将是埃默里·尤德尔中心的重要组成部分。核心将拥有 三个功能：(1)从实验动物身上制备和加工脑组织 项目，(2)用1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP)系统治疗恒河猴 并以标准化的方式评估由此产生的帕金森综合症，以及(3)提供生物统计服务和 为中心内所有项目提供咨询服务。中心将为研究人员提供标准化的组织学服务 使用帕金森综合症的动物模型(项目1-3)。这些服务将包括使用免疫组织化学 和显微镜方法来评估6-羟基多巴胺(6-OHDA)或 MPTP处理的动物(项目1-3)；记录电极轨迹的组织重建(项目1 和2)；分析转基因(视蛋白、荧光蛋白或电压)的表达程度和模式 传感器)在病毒注射后(项目1-3)。这些服务将是解释功能性 并将有助于在项目之间比较和分享结果。同样，所有MPTP处理 将在项目2和项目3中使用的帕金森症猴子将使用标准化的治疗和评估 由核心B人员执行的程序。这将有助于整合和比较这些项目产生的数据。 最后，核心将提供一名生物统计师的服务，该统计师将就统计问题提供咨询和反馈 项目的方方面面。Udall中心的所有四个项目都将从 对实验数据进行统计分析的实验构思和设计。通过提供这些 服务，核心将有助于从研究项目中腾出时间和资源。此外，中心研究 将受益于使用标准化的、完善的解剖学和行为评估技术，以及 生物统计支助将促进所有项目的严密性和可重复性。