The role of mitochondrial metabolism in weight loss

项目摘要 肥胖是心血管（CV）疾病的主要危险因素。各种CV风险因素，如胰岛素抵抗， 血脂异常和炎症与线粒体功能障碍有关。减肥是主要的 建议降低肥胖人群的心脏代谢风险，但保护作用与 体重减轻的幅度，并在体重维持期间随着时间的推移而消退， 体重反弹的人。这项提案旨在研究线粒体适应体重减轻， 可能会降低其预防CV疾病的能力。我们将通过研究高原的适应性来实现这一目标 阶段，尽管努力继续减肥，但进一步减肥变得最小的时间段，以及 体重通常会反弹。平台期目前研究不足，与体重的发生有关。 损失平台期，降低了对心脏代谢风险的保护。 在AIM 1中，我将确定免疫细胞中线粒体在体重减轻平台期的功能差异 从正在减肥的肥胖人群中分离出来。在AIM 2中，我将开发高原的范例- 在饮食诱导的肥胖小鼠中测试是否降低了肝脏线粒体呼吸能力 肌肉和免疫细胞是适应能量消耗（代谢）的潜在机制 适应）在高原，促进体重反弹。对于AIM 3，我将在小鼠模型中确定 加速动脉粥样硬化小鼠是否在高原状态发展更严重的饮食诱导的CMR， 动脉粥样硬化和免疫细胞氧化还原应激。 这项建议将在一个在代谢、肥胖和肥胖方面有强大研究项目的机构进行。 心血管疾病我将接受必要的技术和分析工具方面的最先进的培训， 完成所有目标，包括来自NIH资助的肥胖研究的临床样本， 啮齿类动物和小鼠中致动脉粥样硬化风险的评估。该提案将产生数据，为以下方面奠定基础： 我的独立研究生涯集中在线粒体机制，影响心脏代谢的风险， 肥胖

PROJECT SUMMARY Obesity is a major risk factor for cardiovascular (CV) disease. Various CV risk factors like insulin resistance, dyslipidemia, and inflammation are associated with mitochondrial dysfunction. Weight loss is the main recommendation to reduce cardiometabolic risk for people with obesity, but the protection is proportional to the magnitude of weight lost, and fades over time during weight maintenance with the reversal or worsened risk in those who regain weight. This proposal aims to investigate mitochondrial adaptations with weight loss that might lessen its capacity to protect against CV disease. We will do this by studying adaptation in the plateau phase, a time period when further weight loss becomes minimal despite efforts to continue losing weight, and weight regain often follows. The plateau phase is currently understudied and links the occurrence of a weight loss plateau with diminished protection against cardiometabolic risk. In AIM 1, I will determine functional differences in mitochondria at the weight loss plateau in immune cells isolated from people with obesity undergoing weight loss. In AIM 2, I will develop paradigms of the plateau- state in mice with diet-induced obesity to test whether reduced mitochondrial respiratory capacity in liver muscle and immune cells is an underlying mechanism for adaptation in energy expenditure (metabolic adaptation) at the plateau that promote weight regain. For AIM 3, I will determine in a mouse model of accelerated atherosclerosis whether mice the plateau state develop more severe diet-induced CMR, atherosclerosis, and immune cell redox stress. This proposal will be carried out in an institution with strong research programs in metabolism, obesity, and cardiovascular disease. I will receive state-of-the-art training in techniques and analytical tools necessary for completion of all aims, including clinical samples from NIH-funded obesity studies, indirect calorimetry in rodents, and assessment of atherogenic risk in mice. This proposal will generate data laying the foundation for my independent research career focused on mitochondrial mechanisms that influence cardiometabolic risk in obesity.