ADDING ADDITIONAL FUNDS FOR OPTION PERIOD 1 FOR TOPMED CENTRALIZED OMICS RESOURCE (CORE) YEAR 5

为 TOPMED 集中组学资源（核心）第 5 年的选项期 1 添加额外资金

• 批准号: 10510158
• 负责人: CATHERINE REEVES
• 金额: $ 564.02万
• 依托单位: NEW YORK GENOME CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-28 至 2022-09-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10510158
• 关键词: Bar Codes; Blood; Data; Funding; Globin; Goals; Libraries; Maps; Messenger RNA; National Heart, Lung, and Blood Institute; Preparation; Process; Resources; Sampling; Tissues; Trans-Omics for Precision Medicine; base; indexing; response; transcriptome; transcriptome sequencing

In response to the NHLBI RFP for Transcriptome RNAseq of a minimum of 1,000 samples, we are proposing to profile these samples using the Illumina TruSeq Stranded HT mRNA kit for library preparation, and to sequence the libraries on the Illumina NovaSeq using paired-end 2x100bp reads with eight-base unique dual index barcodes at a minimum depth of 75M mapped reads per blood derived samples and 50M mapped reads per tissue derived or globin-depleted blood derived samples, delivered as CRAM, with a goal of 17,100 samples total. The details of this process are described below, together with data supporting our approach.

应对NHLBI RFP的转录组RNAseq最少1000的样品,我们建议要分析这些样本使用Illumina公司TruSeq滞留HT mRNA装备库的准备,和序列库的Illumina公司NovaSeq使用paired-end 2 x100bp读取eight-base独特的双指数条形码至少75米深度映射读取/血液衍生样品和50 m映射读取/组织派生派生或globin-depleted血液样本,作为补习,目标是总共17100个样本。下面描述了这个过程的细节，以及支持我们方法的数据。