ADDING ADDITIONAL FUNDS FOR OPTION PERIOD 1 FOR TOPMED CENTRALIZED OMICS RESOURCE (CORE) YEAR 5
ADDING ADDITIONAL FUNDS FOR OPTION PERIOD 1 FOR TOPMED CENTRALIZED OMICS RESOURCE (CORE) YEAR 5
为 TOPMED 集中组学资源（核心）第 5 年的选项期 1 添加额外资金
批准号：
10510158
负责人：
CATHERINE REEVES
金额：
$564.02万
依托单位：
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
项目状态：
已结题
起止时间：
2020-09-28 至 2022-09-27
中文摘要
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英文摘要
In response to the NHLBI RFP for Transcriptome RNAseq of a minimum of 1,000 samples, we are proposing to profile these samples using the Illumina TruSeq Stranded HT mRNA kit for library preparation, and to sequence the libraries on the Illumina NovaSeq using paired-end 2x100bp reads with eight-base unique dual index barcodes at a minimum depth of 75M mapped reads per blood derived samples and 50M mapped reads per tissue derived or globin-depleted blood derived samples, delivered as CRAM, with a goal of 17,100 samples total. The details of this process are described below, together with data supporting our approach.
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